Recombinant Mouse CD27 Ligand/TNFSF7 Protein

Carrier Free

Catalog # Availability Size / Price Qty
783-CL-050/CF

With Carrier

Catalog # Availability Size / Price Qty
783-CL-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (2)
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Recombinant Mouse CD27 Ligand/TNFSF7 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to stimulate mouse T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 1.25-5.0 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD27 Ligand/TNFSF7 protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Mouse CD27 Ligand
(Gln47 - Pro195)
Accession # Q05A52
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
19.2 kDa
SDS-PAGE
30 kDa, reducing conditions

Product Datasheets

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783-CL (with carrier)

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783-CL/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

783-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

783-CL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD27 Ligand/TNFSF7

CD27 ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7 (1, 2). Mouse CD27L cDNA encodes a 195 amino acid (aa) residue protein with a 23 aa N-terminal cytoplasmic domain, a 21 transmembrane domain and a 151 aa C-terminal extracellular domain. Mouse and human CD27L share approximated 56% aa sequence identity. By analogy to other TNFSF ligands, CD27L is expected to exist as non-covalent homotrimers. The expression of CD27L is highly regulated at the transcription and posttranslational level (3). CD27L cell surface expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. Although CD27L expression is not detected on human dendritic cells (DC), membrane expression on mature mouse DCs has been reported. CD27L expression is also present in the thymus medulla in both human and mouse. CD27L interacts with CD27, a member of the TNF receptor superfamily that is expressed on natural killer (NK) cells and subsets of T and B cells (2, 4, 5). Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (2). Ligation of CD27 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses (5, 6). On NK cells, ligation of CD27 induces proliferation and IFN-gamma production (4).

References
  1. Hitzen, R.Q. et al. (1994) Immunol. Today 15:307.
  2. Croft, M. (2003) Nature Reviews Immunol. 3:609.
  3. Tesselaar, K. et al. (2003) J. Immunol. 169:33.
  4. Takeda, K. et al. (2000) J. Immunol. 164:1741.
  5. Kobata, T.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11249.
  6. Raman, V.S. et al. (2003) J. Immunol. 171:5876.
Entrez Gene IDs
970 (Human); 21948 (Mouse); 301132 (Rat)
Alternate Names
CD27 Ligand; CD27-L; CD27LCD27 ligand; CD27LG; CD70 molecule; CD70; Ki-24 antigen; surface antigen CD70; TNFSF7; TNFSF7CD70 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7

Citations for Recombinant Mouse CD27 Ligand/TNFSF7 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y
    Biochem. Biophys. Res. Commun., 2009-05-04;384(3):296-300.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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