Recombinant Mouse CD300a/LMIR1 Fc Chimera Protein, CF

R&D Systems | Catalog # 1186-LM

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CD300a/LMIR1 Fc Chimera Protein (1186-LM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

BAC80268.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
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Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD300a/LMIR1 protein
Mouse CD300a
(Leu28-Arg183)
Accession # BAC80268.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu28

Predicted Molecular Mass

43.7 kDa (monomer)

SDS-PAGE

56-66 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
The ED50 for this effect is 1‑5 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

1186-LM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD300a/LMIR1

LMIR1, also known as CD300a, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse LMIR1 consists of a 158 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 112 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2, 3). Within the ECD, mouse LMIR1 shares 40% and 66% aa sequence identity with human and rat LMIR1, respectively. Alternate splicing generates an additional mouse LMIR1 isoform with a 4 aa deletion following the Ig-like domain (3). In mouse, LMIR1 is expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells (2-4). Antibody
cross‑linking of LMIR1 induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5-7). Cross‑linking of LMIR1 to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (4, 8‑10). LMIR1 cross‑linking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 7, 9).

References

  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
  3. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
  4. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.
  5. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
  6. Munitz, A. et al. (2006) Blood 107:1996.
  7. Bachelet, I. et al. (2005) J. Immunol. 175:7989.
  8. Bachelet, I. et al. (2008) J. Immunol. 180:6064.
  9. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
  10. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.

Alternate Names

CD300a, CLM8, CMRF-35H, IGSF12, IRC1, IRC2, IRp60, LMIR1, MAIR-I, Mcipr1, NKRL, Pigr4

Entrez Gene IDs

11314 (Human); 217303 (Mouse)

Gene Symbol

CD300A

UniProt

BAC80268.1

Additional CD300a/LMIR1 Products

Product Documents for Recombinant Mouse CD300a/LMIR1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD300a/LMIR1 Fc Chimera Protein, CF

For research use only

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