Recombinant Mouse CD300a/LMIR1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1186-LM-050
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Mouse CD300a/LMIR1 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 1‑5 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse CD300a/LMIR1 protein
Mouse CD300a
(Leu28-Arg183)
Accession # BAC80268.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43.7 kDa (monomer)
SDS-PAGE
56-66 kDa, reducing conditions

Product Datasheets

1186-LM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD300a/LMIR1

LMIR1, also known as CD300a, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse LMIR1 consists of a 158 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 112 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2, 3). Within the ECD, mouse LMIR1 shares 40% and 66% aa sequence identity with human and rat LMIR1, respectively. Alternate splicing generates an additional mouse LMIR1 isoform with a 4 aa deletion following the Ig-like domain (3). In mouse, LMIR1 is expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells (2-4). Antibody
cross‑linking of LMIR1 induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5-7). Cross‑linking of LMIR1 to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (4, 8‑10). LMIR1 cross‑linking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 7, 9).

References
  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
  3. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
  4. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.
  5. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
  6. Munitz, A. et al. (2006) Blood 107:1996.
  7. Bachelet, I. et al. (2005) J. Immunol. 175:7989.
  8. Bachelet, I. et al. (2008) J. Immunol. 180:6064.
  9. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
  10. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.
Entrez Gene IDs
11314 (Human); 217303 (Mouse)
Alternate Names
CD300a antigenCMRF35H9; CD300a molecule; CD300a; CLM8; CLM-8; CMRF35H leukocyte immunoglobulin-like receptor; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF-35-H9CD300 antigen-like family member A; CMRF35HIRp60; CMRF35-like molecule 8; IGSF12; IGSF12NK inhibitory receptor; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Primary Antibodies

Human IgG1 Antibody

Human IgG1 Antibody

Recombinant Proteins

Reconstitution Buffers

Reviews for Recombinant Mouse CD300a/LMIR1 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse CD300a/LMIR1 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Mouse CD300a/LMIR1 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review