Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
2519-CD-050

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Recombinant Mouse CD36/SR-B3 Fc Chimera Protein Binding Activity
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Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. In a Goat anti-Human IgG Fc antibody (Catalog # G-102-C) coated plate, Recombinant Mouse CD36/SR‑B3 Fc Chimera using at 1 µg/mL binds Recombinant Human Thrombospondin-2 (Catalog # 1635-T2) with an ED50 of 0.06-0.6 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse CD36/SR-B3 protein
Mouse CD36
(Gly30-Lys439)
Accession # Q3UAI3
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Gly30
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
72.9 kDa (monomer)
SDS-PAGE
100-130 kDa, reducing conditions

Product Datasheets

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2519-CD

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2519-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Mouse CD36/SR-B3 Fc Chimera Protein Binding Activity View Larger

Recombinant Mouse CD36/SR-B3 Fc Chimera (Catalog # 2519-CD) is used at 1 µg/mL, Recombinant Human Thrombospondin-2 (Catalog # 1635-T2) binds with an ED50 of 0.06-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: CD36/SR-B3

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N-terminal cytoplasmic domain, a 22 aa N-terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C-terminal transmembrane segment, and a 9 aa C-terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C-terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N-terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 is 94%, 92%, 84% and 84% aa identical to the extracellular loops of rat, hamster, human and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells and hematopoietic cells such as RBC’s, platelets and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

References
  1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:795. 
  2. Silverstein, R.L. and M. Febbraio (2000) Curr. Opin. Lipid. 11:483. 
  3. Gruarin, P. et al. (2000) Biochem. Biophys. Res. Commun. 275:446. 
  4. Endemann, G. et al. (1993) J. Biol. Chem. 268:11811. 
  5. Malaud, E. et al. (2002) Biochem. J. 364:507.
  6. Tao, N. et al. (1996) J. Biol. Chem. 271:22315.
  7. Daviet, L. et al. (1997) Thromb. Haemost. 78:897.
  8. Simantov, R. and R.L. Silverstein (2003) Front. Biosci. 8:s874.
Long Name
Scavenger Receptor Class B, Member 3
Entrez Gene IDs
948 (Human); 12491 (Mouse)
Alternate Names
BDPLT10; CD_antigen: CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; CHDS7; cluster determinant 36; Collagen R; FAT; Fatty acid translocase; Glycoprotein IIIb; GP3B; GP4; GPIIIb; GPIV; PAS IV; PAS-4 protein; PAS-4; PASIV; Platelet collagen receptor; platelet glycoprotein 4; SCARB3; scavenger receptor class B, member 3; SRB3; SR-B3; Thrombospondin R; Thrombospondin receptor

Citation for Recombinant Mouse CD36/SR-B3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages.
    Authors: Biedron R, Konopinski M, Marcinkiewicz J, Jozefowski S
    PLoS ONE, 2015;10(4):e0123293.
    Species: Mouse
    Sample Types: Protein
    Applications: Binding Assay

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