Recombinant Mouse CD8 alpha Protein, CF

Name: Recombinant Mouse CD8 alpha Protein, CF
Brand: R&D Systems
SKU: 9135-CD
Price: 605 USD
Availability: InStock

R&D Systems | Catalog # 9135-CD

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
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Key Product Details

R&D Systems NS0-derived Recombinant Mouse CD8 alpha Protein (9135-CD)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P01731

Structure / Form

Noncovalently-linked homodimer

Applications

Bioactivity

View all CD8 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD8 protein
Lys28-Tyr196, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys28

Predicted Molecular Mass

20 kDa

SDS-PAGE

32-41 kDa, reducing conditions

Activity

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.
The ED₅₀ for this effect is 1-5 μg/mL.

Scientific Data Images for Recombinant Mouse CD8 alpha Protein, CF

Recombinant Mouse CD8 alpha Protein Bioactivity

Immobilized Recombinant Mouse CD8 alpha (Catalog # 9135-CD) supports the adhesion of Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 1-5 μg/mL.

Formulation, Preparation, and Storage

9135-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Loading...
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD8

CD8, also known as Ly-2 or Leu-2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes (1, 2). CD8 alpha is expressed on double positive (CD4⁺CD8⁺) thymocytes and mature CD8⁺ cytolytic T cells (CTL) (3-5), intraepithelial lymphocytes (IEL) (6), some gamma-delta T cells (7), subsets of thymic and splenic dendritic cells (DC) (8), and peritoneal mast cells (9). It can form disulfide linked homodimers or heterodimers with CD8 beta (10). Thymic CD8⁺ DC express primarily alpha beta heterodimers, while splenic CD8⁺ DC primarily express alpha alpha homodimers (8). CD8 alpha ⁺ DC can present viral antigenic peptides in complex with MHC I and prime CTL responses (11). The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (12). Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively.