Recombinant Mouse CD8 alpha Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 1-5 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived mouse CD8 protein
    Lys28-Tyr196, with a C-terminal 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Lys28
  • Structure / Form
    Noncovalently-linked homodimer
  • Predicted Molecular Mass
    20 kDa
  • SDS-PAGE
    32-41 kDa, reducing conditions
Product Datasheets

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9135-CD
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 500 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
Immobilized Recombinant Mouse CD8 alpha (Catalog # 9135-CD) supports the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 1-5 μg/mL.
Background: CD8
CD8, also known as Ly-2 or Leu-2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes (1, 2). CD8 alpha is expressed on double positive (CD4+CD8+) thymocytes and mature CD8+ cytolytic T cells (CTL) (3-5), intraepithelial lymphocytes (IEL) (6), some gamma-delta T cells (7), subsets of thymic and splenic dendritic cells (DC) (8), and peritoneal mast cells (9). It can form disulfide linked homodimers or heterodimers with CD8 beta (10). Thymic CD8+ DC express primarily alpha beta heterodimers, while splenic CD8+ DC primarily express alpha alpha homodimers (8). CD8 alpha + DC can present viral antigenic peptides in complex with MHC I and prime CTL responses (11). The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (12). Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively.
  • References:
    1. Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
    2. Cole, D.K. et al. (2012) Immunology 137:139.
    3. Germain, R.N. (2002) Nat. Rev. Immunol. 2:309.
    4. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
    5. Nakayama, K. et al. (1994) Science 263:1131.
    6. Wang, J. and J.R. Klein (1994) Science 265:1860.
    7. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
    8. Vremec, D. et al. (1992) J. Exp. Med. 176:47.
    9. Lin, T.-J. et al. (1998) J. Immunol. 161:6265.
    10. Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
    11. Belz, G.T. et al. (2004) J. Immunol. 172:1996.
    12. Nakauchi, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5126.
  • Entrez Gene IDs:
    925 (Human); 12525 (Mouse); 24930 (Rat); 493799 (Feline)
  • Alternate Names:
    CD8; CD8A
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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 28  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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