Recombinant Mouse CD96 Protein, CF

His-tag version
Catalog # Availability Size / Price Qty
5690-CD-050

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Recombinant Mouse CD96 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When recombinant mouse (rm) CD155 is coated at 5 μg/mL (100 μL/well), the concentration of rmCD96 that produces 50% optimal binding response is found to be approximately 0.015-0.075 μg/mL.

Source
Mouse myeloma cell line, NS0-derived mouse CD96 protein
Met1-Met536, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu25
Predicted Molecular Mass
57.2 kDa
SDS-PAGE
135-155 kDa, reducing conditions

Product Datasheets

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5690-CD

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5690-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD96

Mouse CD96, also known as Tactile (T cell‑activated increased late expression), is a 160 kDa type I transmembrane glycoprotein of the Ig superfamily that shows peak expression 6 ‑ 9 days after activation of T, NK, and a subpopulation of B cells (1, 2). CD96 is also frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). Low expression of adhesive human CD96 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 5). Mouse CD96 cDNA encodes 602 amino acids (aa) including a 21 aa signal peptide, a 515 aa extracellular domain (ECD) that contains two V‑type and one C‑type Ig‑like  domain, a 21 aa transmembrane sequence, and a 45 aa cytoplasmic domain (1). Within the ECD, mouse CD96 shares 55% and 79% aa sequence identity with human CD96v2 and rat CD96, respectively (6). The ECD is highly N- and O‑glycosylated (1). Humans, but not mice, express a splice variant with a 16 aa insert in the second V‑type domain called CD96v1 (2). In mice, a truncated, potentially secreted 437 aa isoform is reported (7). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (8). The most N‑terminal Ig‑like domain of human or mouse CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species‑specific (2, 6, 9). Mouse CD96 also binds nectin-1 (6). On NK cells, co‑stimulatory CD96 and DNAM‑1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various nectins (10, 11). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (9, 10).

References
  1. Wang, P.L. et al. (1992) J. Immunol. 148:2600.
  2. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
  3. Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008.
  4. Xie, W. et al. (2010) Cytometry A 77:840.
  5. Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835.
  6. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
  7. Protein Accession # BAE32358.
  8. Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207.
  9. Fuchs, A. et al. (2004) J. Immunol. 172:3394.
  10. Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895.
  11. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
Entrez Gene IDs
10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)
Alternate Names
CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile

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