Recombinant Mouse CXCL10/IP-10/CRG-2 Protein

Carrier Free

Catalog # Availability Size / Price Qty
466-CR-050/CF

With Carrier

Catalog # Availability Size / Price Qty
466-CR-010
466-CR-050

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R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (17)
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Reviews (1)

Recombinant Mouse CXCL10/IP-10/CRG-2 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED50 for this effect is 0.05-0.3 µg/mL.
Source
E. coli-derived mouse CXCL10/IP-10/CRG-2 protein
Ile22-Pro98, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
8.8 kDa

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466-CR (with carrier)

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466-CR/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

466-CR

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

466-CR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: CXCL10/IP-10/CRG-2

The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has since been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Recently, human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

References
  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Vanguri, P. (1996) J. Neuroimmunol. 56:35.
  3. Sgadari, C. et al. (1996) Blood, 87:3877.
Entrez Gene IDs
3627 (Human); 15945 (Mouse)
Alternate Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

Citations for Recombinant Mouse CXCL10/IP-10/CRG-2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Dissection of &alpha4&beta7 integrin regulation by Rap1 using novel conformation-specific monoclonal anti-&beta7 antibodies
    Authors: T Sato, S Ishihara, R Marui, J Takagi, K Katagiri
    Sci Rep, 2020;10(1):13221.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Prion protein N1 cleavage peptides stimulate microglial interaction with surrounding cells
    Authors: JA Carroll, BR Groveman, K Williams, R Moore, B Race, CL Haigh
    Sci Rep, 2020;10(1):6654.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy
    Authors: YP Xu, L Lv, Y Liu, MD Smith, WC Li, XM Tan, M Cheng, Z Li, M Bovino, J Aubé, Y Xiong
    J. Clin. Invest., 2019;130(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity
    Authors: S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming
    Front Microbiol, 2019;10(0):1421.
    Species: Mouse
    Sample Types:
    Applications: Binding Assay
  5. Induced Regulatory T Cells Superimpose Their Suppressive Capacity with Effector T Cells in Lymph Nodes via Antigen-Specific S1p1-Dependent Egress Blockage
    Authors: S Geng, Y Zhong, X Zhou, G Zhao, X Xie, Y Pei, H Liu, H Zhang, Y Shi, B Wang
    Front Immunol, 2017;8(0):663.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. A novel function of CXCL10 in mediating monocyte production of proinflammatory cytokines
    Authors: Q Zhao, T Kim, J Pang, W Sun, X Yang, J Wang, Y Song, H Zhang, H Sun, V Rangan, S Deshpande, H Tang, ME Cvijic, R Westhouse, T Olah, J Xie, M Struthers, L Salter-Cid
    J. Leukoc. Biol., 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. NK cell heparanase controls tumor invasion and immune surveillance
    Authors: EM Putz, AJ Mayfosh, K Kos, DS Barkauskas, K Nakamura, L Town, KJ Goodall, DY Yee, IK Poon, N Baschuk, F Souza-Fons, MD Hulett, MJ Smyth
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Protected graft copolymer-formulated fibroblast growth factors mitigate the lethality of partial body irradiation injury
    Authors: GM Castillo, A Nishimoto-, CC Jones, KK Kabirov, A Zakharov, AV Lyubimov
    PLoS ONE, 2017;12(2):e0171703.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA (Standard)
  9. CXCL10 triggers early microglial activation in the cuprizone model.
    Authors: Clarner T, Janssen K, Nellessen L, Stangel M, Skripuletz T, Krauspe B, Hess F, Denecke B, Beutner C, Linnartz-Gerlach B, Neumann H, Vallieres L, Amor S, Ohl K, Tenbrock K, Beyer C, Kipp M
    J Immunol, 2015;194(7):3400-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. TRIF-dependent innate immune activation is critical for survival to neonatal gram-negative sepsis.
    Authors: Cuenca A, Joiner D, Gentile L, Cuenca A, Wynn J, Kelly-Scumpia K, Scumpia P, Behrns K, Efron P, Nacionales D, Lui C, Wallet S, Reeves W, Mathews C, Moldawer L
    J Immunol, 2015;194(3):1169-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Monocyte- and Neutrophil-Derived CXCL10 Impairs Efficient Control of Blood-Stage Malaria Infection and Promotes Severe Disease.
    Authors: Ioannidis L, Nie C, Ly A, Ryg-Cornejo V, Chiu C, Hansen D
    J Immunol, 2015;196(3):1227-38.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA (Standard)
  12. Interferon-gamma Promotes Inflammation and Development of T-Cell Lymphoma in HTLV-1 bZIP Factor Transgenic Mice.
    Authors: Mitagami Y, Yasunaga J, Kinosada H, Ohshima K, Matsuoka M
    PLoS Pathog, 2015;11(8):e1005120.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. CXCR3 promotes plaque formation and behavioral deficits in an Alzheimer's disease model.
    Authors: Krauthausen M, Kummer M, Zimmermann J, Reyes-Irisarri E, Terwel D, Bulic B, Heneka M, Muller M
    J Clin Invest, 2015;125(1):365-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration.
    Authors: Fujihara Y, Takato T, Hoshi K
    Stem Cells, 2014;32(5):1208-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Early differentiated CD138(high) MHCII+ IgG+ plasma cells express CXCR3 and localize into inflamed kidneys of lupus mice.
    Authors: Lacotte S, Decossas M, Le Coz C, Brun S, Muller S, Dumortier H
    PLoS ONE, 2013;8(3):e58140.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Chemokine receptor CXCR3 promotes growth of glioma.
    Authors: Liu C, Luo D, Reynolds BA
    Carcinogenesis, 2011;32(2):129-37.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. TLR2-mediated production of IL-27 and chemokines by respiratory epithelial cells promotes bleomycin-induced pulmonary fibrosis in mice.
    Authors: Kim HS, Go H, Akira S, Chung DH
    J. Immunol., 2011;187(8):4007-17.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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Recombinant Mouse CXCL10/IP-10/CRG-2 Protein
By Anonymous on 08/02/2016
Application: Cell migration/motility