Recombinant Mouse Dkk-1 N-Terminal Fragment Protein, CF

Catalog # Availability Size / Price Qty
9839-DK-050
Recombinant Mouse Dkk-1 N-Terminal Fragment Protein Binding Activity
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Recombinant Mouse Dkk-1 N-Terminal Fragment Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Dkk‑1 N-Terminal Fragment is coated at 1 μg/mL, Mouse CKAP4/p63 (Catalog # 9734-CK) binds with an ED50 of 15-90 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Dkk-1 protein
Met1-Ser144, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser30 & Thr32
Predicted Molecular Mass
13 kDa
SDS-PAGE
13-22 kDa, reducing conditions

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9839-DK

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9839-DK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse Dkk-1 N-Terminal Fragment Protein Binding Activity View Larger

When Recombinant Mouse Dkk-1 N-Terminal Fragment (Catalog # 9839-DK) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Mouse CKAP4/p63 (Catalog # 9734-CK) binds with an ED50 of 15-90 ng/mL.

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Reconstitution Calculator

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Background: Dkk-1

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Mature mouse Dkk-1 is a 40 kDa secreted glycoprotein that consists of two conserved cysteine-rich domains (CRDs), CRD1 (N-terminal) and CRD2 (C-terminal),  separated by a linker region (1, 3). Within the N-terminal fragment [amino acids (aa) 32-144] that includes the CRD1, mouse Dkk-1 shares 98% and 85% aa sequence identity with rat and human Dkk-1, respectively.  Dkk-1 antagonizes Wnt/beta-catenin signaling, an activity for which the C-terminal CRD2 is both necessary and sufficient (4, 5), while the N-terminal CRD1 was required for binding to CKAP4 (6). However, crystallographic studies have shown that Dkk-1 interacts with LRP-6 as a bipartite inhibitor, with both CRDs binding the extracellular domain of LRP-6 simultaneously (3, 7-9). Mechanistically, Dkk-1 has been shown to promote the internalization and degradation of LRP-6, but mouse Dkk-1 may inhibit LRP-6 independently of this process (10, 11). Mice lacking Dkk-1 die at birth, lack anterior head structures, and have limb malformations (12). Accordingly, bone mass in mice has been found to be inversely proportional to Dkk-1 levels (13). Reduced Dkk-1 expression causes head, limb, and vertebrae defects in mice (14). Conversely, transgenic mice that overexpress Dkk-1 develop osteopenia (15). In addition to bone homeostasis, Dkk-1 expression may be required for neural differentiation of mouse embryonic stem (ES) cells (16, 17). Dkk-1 also likely has a complex role in cancer, as it has been shown to act as a tumor suppressor and also to promote metastasis (18).

References
  1. Glinka, A. et al. (1998) Nature 391:357.
  2. Niehrs, C. (2006) Oncogene 25:7469.
  3. Ahn, V.E. et al. (2011) Dev. Cell 21:862.
  4. Mao, B. et al. (2001) Nature 411:321.
  5. Brott, B.K. and S.Y. Sokol (2002) Mol. Cell. Biol. 22:6100.
  6. Kimura, H. et al. (2016) J. Clin. Invest. 126:7.
  7. Chen, S. et al. (2011) Dev. Cell 21:848.
  8. Bourhis, E. et al. (2011) Structure 19:1433.
  9. Cheng, Z. et al. (2011) Nat. Struct. Mol. Biol. 18:1204.
  10. Mao, B. et al. (2002) Nature 417:664.
  11. Semënov, M.V. et al. (2008) J. Biol. Chem. 283:21427.
  12. Mukhopadhyay, M. et al. (2001) Dev. Cell 1:423.
  13. MacDonald, B.T. et al. (2007) Bone 41:331.
  14. MacDonald, B.T. et al. (2004) Development 131:2543.
  15. Li, J. et al. (2006) Bone 39:754.
  16. Verani, R. et al. (2007) J. Neurochem. 100:242.
  17. Cajánek, L. et al. (2009) Stem Cells 27:2917.
  18. Menezes, M.E. et al. (2012) Int. J. Cancer 130:1477.
Long Name
Dickkopf-1
Entrez Gene IDs
22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)
Alternate Names
dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like

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