Recombinant Mouse Dkk-1 Protein

Carrier Free

Catalog # Availability Size / Price Qty
5897-DK-010/CF

With Carrier

Catalog # Availability Size / Price Qty
5897-DK-010
Recombinant Mouse Dkk-1 Protein Bioactivity
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Product Details
Citations (45)
FAQs
Reviews (4)

Recombinant Mouse Dkk-1 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-1 (Catalog # 5897-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 for this effect is 10-60 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Dkk-1 protein
Ser30-His272
Accession #
N-terminal Sequence
Analysis
Ser30 & Thr32
Predicted Molecular Mass
26.1 kDa
SDS-PAGE
35-40 kDa, reducing conditions

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5897-DK (with carrier)

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5897-DK/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5897-DK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5897-DK/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Dkk-1 Protein Bioactivity View Larger

Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 5897-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED50 for this effect is 10-60 ng/mL (orange circles).

SDS-PAGE Recombinant Mouse Dkk-1 Protein SDS-PAGE View Larger

1 µg/lane of Recombinant Mouse Dkk-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 35-40 kDa. Multiple bands in gel are due to variable glycosylation.

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Background: Dkk-1

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1-3). Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk‑1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low‑density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk-1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

References
  1. Glinka, A. et al. (1998) Nature 391:357.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Pinzone, J.J. et al. (2009) Blood 113:517.
  11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.
Long Name
Dickkopf-1
Entrez Gene IDs
22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)
Alternate Names
dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like

Citations for Recombinant Mouse Dkk-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

45 Citations: Showing 1 - 10
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  1. CCN1 interacts with integrins to regulate intestinal stem cell proliferation and differentiation
    Authors: JH Won, JS Choi, JI Jun
    Nature Communications, 2022;13(1):3117.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  2. Coordinate control of basal epithelial cell fate and stem cell maintenance by core EMT transcription factor Zeb1
    Authors: Y Han, A Villarreal, G Gutierrez, Q Nguyen, P Sun, T Wu, B Sui, G Berx, T Brabletz, K Kessenbroc, YA Zeng, K Watanabe, X Dai
    Cell Reports, 2022;38(2):110240.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Wnt3a upregulation is involved in TGF&beta1-induced cardiac hypertrophy
    Authors: T Li, X Weng, S Cheng, D Wang, G Cheng, H Gao, Y Li
    Cytokine, 2020;0(0):155376.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Shaking culture enhances chondrogenic differentiation of mouse induced pluripotent stem cell constructs
    Authors: P Limraksasi, Y Kosaka, M Zhang, N Horie, T Kondo, H Okawa, M Yamada, H Egusa
    Sci Rep, 2020;10(1):14996.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Engraftment potential of maternal adipose-derived stem cells for fetal transplantation
    Authors: A Kawashima, R Yasuhara, R Akino, K Mishima, M Nasu, A Sekizawa
    Heliyon, 2020;6(3):e03409.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells
    Authors: W Liang, P Han, EH Kim, J Mak, R Zhang, AG Torrente, JI Goldhaber, E Marbán, HC Cho
    Stem Cells, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. SDF?1/CXCR4 induces epithelial?mesenchymal transition through activation of the Wnt/??catenin signaling pathway in rat chronic allograft nephropathy
    Authors: H Tang, Y Xu, Z Zhang, S Zeng, W Dong, W Jiao, X Hu
    Mol Med Rep, 2019;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Tankyrase inhibition preserves osteoarthritic cartilage by coordinating cartilage matrix anabolism via effects on SOX9 PARylation
    Authors: S Kim, S Han, Y Kim, HS Kim, YR Gu, D Kang, Y Cho, H Kim, J Lee, Y Seo, MJ Chang, CB Chang, SB Kang, JH Kim
    Nat Commun, 2019;10(1):4898.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Cross-Inhibition of Norrin and TGF-? Signaling Modulates Development of Retinal and Choroidal Vasculature
    Authors: R Seitz, G Weber, S Albrecht, R Fuchshofer, ER Tamm, A Ohlmann
    Invest. Ophthalmol. Vis. Sci., 2018;59(6):2240-2251.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. miRNA-431 Prevents Amyloid-?-Induced Synapse Loss in Neuronal Cell Culture Model of Alzheimer's Disease by Silencing Kremen1
    Authors: SP Ross, KE Baker, A Fisher, L Hoff, ES Pak, AK Murashov
    Front Cell Neurosci, 2018;12(0):87.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Klotho expression is a prerequisite for proper muscle stem cell function and regeneration of skeletal muscle
    Authors: HE Ahrens, J Huettemeis, M Schmidt, C Kaether, J von Maltza
    Skelet Muscle, 2018;8(1):20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration
    Authors: K Wilk, SA Yeh, LJ Mortensen, S Ghaffariga, CM Lombardo, SH Bassir, ZA Aldawood, CP Lin, G Intini
    Stem Cell Reports, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma
    Authors: T Tammela, FJ Sanchez-Ri, NM Cetinbas, K Wu, NS Joshi, K Helenius, Y Park, R Azimi, NR Kerper, RA Wesselhoef, X Gu, L Schmidt, M Cornwall-B, ÖH Yilmaz, W Xue, P Katajisto, A Bhutkar, T Jacks
    Nature, 2017;545(7654):355-359.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Angiogenesis in a 3D model containing adipose tissue stem cells and endothelial cells is mediated by canonical Wnt signaling
    Authors: X Cai, J Xie, Y Yao, X Cun, S Lin, T Tian, B Zhu, Y Lin
    Bone Res, 2017;5(0):17048.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  15. Directed transdifferentiation of M�ller glial cells to photoreceptors using the sonic hedgehog signaling pathway agonist purmorphamine
    Authors: D Gu, S Wang, S Zhang, P Zhang, G Zhou
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Wnts contribute to neuromuscular junction formation through distinct signaling pathways
    Authors: J Messéant, J Ezan, P Delers, K Glebov, C Marchiol, F Lager, G Renault, F Tissir, M Montcouqui, N Sans, C Legay, L Strochlic
    Development, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. Activation of Wnt3?/?-catenin signal pathway attenuates apoptosis of the cerebral microvascular endothelial cells induced by oxygen-glucose deprivation
    Authors: J Zhang, J Zhang, C Qi, P Yang, X Chen, Y Liu
    Biochem. Biophys. Res. Commun, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Exosomes Mediate Mobilization of Autocrine Wnt10b to Promote Axonal Regeneration in the Injured CNS
    Authors: NG Tassew, J Charish, AP Shabanzade, V Luga, H Harada, N Farhani, P D'Onofrio, B Choi, A Ellabban, PEB Nickerson, VA Wallace, PD Koeberle, JL Wrana, PP Monnier
    Cell Rep, 2017;20(1):99-111.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates
    Authors: MW Susman, EP Karuna, RC Kunz, TS Gujral, AV Cantú, SS Choi, BY Jong, K Okada, MK Scales, J Hum, LS Hu, MW Kirschner, R Nishinakam, S Yamada, DJ Laird, LE Jao, SP Gygi, ME Greenberg, HH Ho
    Elife, 2017;6(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Real-Time Analysis of Endogenous Wnt Signalling in 3D Mesenchymal Stromal Cells
    Stem Cells Int, 2016;2016(0):7132529.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms
    Nat. Med, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  22. RSPOs facilitated HSC activation and promoted hepatic fibrogenesis
    Authors: X Yin, H Yi, L Wang, W Wu, X Wu, L Yu
    Oncotarget, 2016;7(39):63767-63778.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Adverse Effects of Osteocytic Constitutive Activation of ss-Catenin on Bone Strength and Bone Growth.
    Authors: Chen S, Feng J, Bao Q, Li A, Zhang B, Shen Y, Zhao Y, Guo Q, Jing J, Lin S, Zong Z
    J Bone Miner Res, 2015;30(7):1184-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
    Authors: Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G
    PLoS ONE, 2015;10(4):e0124213.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  25. Wnt activity and basal niche position sensitize intestinal stem and progenitor cells to DNA damage.
    Authors: Tao S, Tang D, Morita Y, Sperka T, Omrani O, Lechel A, Sakk V, Kraus J, Kestler H, Kuhl M, Rudolph K
    EMBO J, 2015;34(5):624-40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  26. Hmga2 is required for canonical WNT signaling during lung development.
    Authors: Singh I, Mehta A, Contreras A, Boettger T, Carraro G, Wheeler M, Cabrera-Fuentes H, Bellusci S, Seeger W, Braun T, Barreto G
    BMC Biol, 2014;12(0):21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Dose-dependent effect of estrogen suppresses the osteo-adipogenic transdifferentiation of osteoblasts via canonical Wnt signaling pathway.
    Authors: Gao B, Huang Q, Lin Y, Wei B, Guo Y, Sun Z, Wang L, Fan J, Zhang H, Han Y, Li X, Shi J, Liu J, Yang L, Luo Z
    PLoS ONE, 2014;9(6):e99137.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. The hippo pathway effector Yap controls patterning and differentiation of airway epithelial progenitors.
    Authors: Mahoney J, Mori M, Szymaniak A, Varelas X, Cardoso W
    Dev Cell, 2014;30(2):137-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  29. Hepatocyte growth factor (Hgf) stimulates low density lipoprotein receptor-related protein (Lrp) 5/6 phosphorylation and promotes canonical Wnt signaling.
    Authors: Koraishy F, Silva C, Mason S, Wu D, Cantley L
    J Biol Chem, 2014;289(20):14341-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Adipocytic cells augment the support of primitive hematopoietic cells in vitro but have no effect in the bone marrow niche under homeostatic conditions.
    Authors: Spindler, Tassja J, Tseng, Alan W, Zhou, Xiaoying, Adams, Gregor B
    Stem Cells Dev, 2014;23(4):434-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Both canonical and non-canonical Wnt signaling independently promote stem cell growth in mammospheres.
    Authors: Many A, Brown A
    PLoS ONE, 2014;9(7):e101800.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Phosphatase WIP1 regulates adult neurogenesis and WNT signaling during aging.
    Authors: Zhu, Yunhua, Demidov, Oleg N, Goh, Amanda M, Virshup, David M, Lane, David P, Bulavin, Dmitry V
    J Clin Invest, 2014;124(7):3263-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Wnt signaling in form deprivation myopia of the mice retina.
    Authors: Ma M, Zhang Z, Du E, Zheng W, Gu Q, Xu X, Ke B
    PLoS ONE, 2014;9(4):e91086.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  34. Recombinant Wnt3a and Wnt5a elicit macrophage cytokine production and tolerization to microbial stimulation via Toll-like receptor 4.
    Authors: Yu C, Nguyen T, Irvine K, Sweet M, Frazer I, Blumenthal A
    Eur J Immunol, 2014;44(5):1480-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Integration of multiple signaling regulates through apoptosis the differential osteogenic potential of neural crest-derived and mesoderm-derived Osteoblasts.
    Authors: Li S, Meyer N, Quarto N, Longaker M
    PLoS ONE, 2013;8(3):e58610.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  36. A modular gain-of-function approach to generate cortical interneuron subtypes from ES cells.
    Authors: Au E, Ahmed T, Karayannis T, Biswas S, Gan L, Fishell G
    Neuron, 2013;80(5):1145-58.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Cripto-1 enhances the canonical Wnt/β-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors.
    Cell Signal, 2013;25(1):178-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. A network of heterochronic genes including Imp1 regulates temporal changes in stem cell properties.
    Authors: Nishino, Jinsuke, Kim, Sunjung, Zhu, Yuan, Zhu, Hao, Morrison, Sean J
    Elife, 2013;2(0):e00924.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  39. Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel.
    Authors: Jin L, Feng T, Shih H, Zerda R, Luo A, Hsu J, Mahdavi A, Sander M, Tirrell D, Riggs A, Ku H
    Proc Natl Acad Sci U S A, 2013;110(10):3907-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Mutational analysis of sclerostin shows importance of the flexible loop and the cystine-knot for Wnt-signaling inhibition.
    Authors: Boschert V, van Dinther M, Weidauer S, van Pee K, Muth E, ten Dijke P, Mueller T
    PLoS ONE, 2013;8(11):e81710.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration.
    Authors: Tran, Thanh H, Shi, Xiaofeng, Zaia, Joseph, Ai, Xingbin
    J Biol Chem, 2012;287(39):32651-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Coordination of epithelial branching and salivary gland lumen formation by Wnt and FGF signals.
    Authors: Patel N, Sharpe PT, Miletich I
    Dev. Biol., 2011;358(1):156-67.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Wnt signaling in amygdala-dependent learning and memory.
    Authors: Maguschak KA, Ressler KJ
    J. Neurosci., 2011;31(37):13057-67.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  44. Distinct modes of inhibition by sclerostin on bone morphogenetic protein and Wnt signaling pathways.
    Authors: Krause C, Korchynskyi O, de Rooij K, Weidauer SE, de Gorter DJ, van Bezooijen RL, Hatsell S, Economides AN, Mueller TD, Lowik CW, ten Dijke P
    J. Biol. Chem., 2010;285(53):41614-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Chemokine-Like Receptor 1 Is a Novel Wnt Target Gene that Regulates Mesenchymal Stem Cell Differentiation.
    Authors: Muruganandan S, Govindarajan R, McMullen N, Sinal C
    Stem Cells, 0;35(3):711-724.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Mouse Dkk-1 Protein

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Recombinant Mouse Dkk-1 Protein
By Anonymous on 04/17/2020
Application: Media additive for protein or antibody production

Recombinant Mouse Dkk-1 Protein
By Anonymous on 10/13/2018
Application: CellProlif

Recombinant Mouse Dkk-1 Protein
By Anonymous on 10/04/2016
Application: In vitro bioactivity in cell culture
Reason for Rating: It works as expected

Recombinant Mouse Dkk-1 Protein
By Ian White on 05/17/2016
Application: CellProlif
Reason for Rating: WORKED AS EXPECTED