Recombinant Mouse Dkk-1 Protein

(29 citations)
(2 Reviews)
  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ND50 for this effect is 0.02‑0.1 μg/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN). Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-1 (Catalog # 5897-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 for this effect is 10-60 ng/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Ser30-His272
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser30 & Thr32
  • Predicted Molecular Mass
    26.1 kDa
  • SDS-PAGE
    35-40 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5897-DK
 
5897-DK/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 5897‑DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED50 for this effect is
10‑60 ng/mL (orange circles).
Recombinant Mouse Dkk-1 (Catalog # 5439-DK) inhibits Wnt-3a-induced alkaline phosphatase production by the MC3T3‑E1 mouse preosteoblast cell line. The ED50 for this effect is 0.02-0.1 µg/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).
1 µg/lane of Recombinant Mouse Dkk-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 35-40 kDa. Multiple bands in gel are due to variable glycosylation.
Background: Dkk-1

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1-3). Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk‑1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low‑density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk-1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

  • References:
    1. Glinka, A. et al. (1998) Nature 391:357.  
    2. Niehrs, C. (2006) Oncogene 25:7469. 
    3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
    4. Mao, B. et al. (2001) Nature 411:321. 
    5. Mao, B. et al. (2002) Nature 417:664.  
    6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
    7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
    8. Li, J. et al. (2006) Bone 36:754.
    9. Verani, R. et al. (2006) J. Neurochem. 101:242.
    10. Pinzone, J.J. et al. (2009) Blood 113:517.
    11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
    12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.
  • Long Name:
    Dickkopf-1
  • Entrez Gene IDs:
    22943 (Human); 13380 (Mouse)
  • Alternate Names:
    dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Exosomes Mediate Mobilization of Autocrine Wnt10b to Promote Axonal Regeneration in the Injured CNS
    Authors: NG Tassew, J Charish, AP Shabanzade, V Luga, H Harada, N Farhani, P D'Onofrio, B Choi, A Ellabban, PEB Nickerson, VA Wallace, PD Koeberle, JL Wrana, PP Monnier
    Cell Rep, 2017;20(1):99-111.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma
    Authors: T Tammela, FJ Sanchez-Ri, NM Cetinbas, K Wu, NS Joshi, K Helenius, Y Park, R Azimi, NR Kerper, RA Wesselhoef, X Gu, L Schmidt, M Cornwall-B, ÖH Yilmaz, W Xue, P Katajisto, A Bhutkar, T Jacks
    Nature, 2017;545(7654):355-359.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Postnatal Calvarial Skeletal Stem Cells Expressing PRX1 Reside Exclusively in the Calvarial Sutures and Are Required for Bone Regeneration
    Authors: K Wilk, SA Yeh, LJ Mortensen, S Ghaffariga, CM Lombardo, SH Bassir, ZA Aldawood, CP Lin, G Intini
    Stem Cell Reports, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. RSPOs facilitated HSC activation and promoted hepatic fibrogenesis
    Authors: X Yin, H Yi, L Wang, W Wu, X Wu, L Yu
    Oncotarget, 2016;7(39):63767-63778.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Real-Time Analysis of Endogenous Wnt Signalling in 3D Mesenchymal Stromal Cells
    Stem Cells Int, 2016;2016(0):7132529.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms
    Nat. Med, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  7. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
    Authors: Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G
    PLoS ONE, 2015;10(4):e0124213.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  8. Adverse Effects of Osteocytic Constitutive Activation of ss-Catenin on Bone Strength and Bone Growth.
    Authors: Chen S, Feng J, Bao Q, Li A, Zhang B, Shen Y, Zhao Y, Guo Q, Jing J, Lin S, Zong Z
    J Bone Miner Res, 2015;30(7):1184-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Wnt activity and basal niche position sensitize intestinal stem and progenitor cells to DNA damage.
    Authors: Tao S, Tang D, Morita Y, Sperka T, Omrani O, Lechel A, Sakk V, Kraus J, Kestler H, Kuhl M, Rudolph K
    EMBO J, 2015;34(5):624-40.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  10. Phosphatase WIP1 regulates adult neurogenesis and WNT signaling during aging.
    Authors: Zhu, Yunhua, Demidov, Oleg N, Goh, Amanda M, Virshup, David M, Lane, David P, Bulavin, Dmitry V
    J Clin Invest, 2014;124(7):3263-73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Both canonical and non-canonical Wnt signaling independently promote stem cell growth in mammospheres.
    Authors: Many A, Brown A
    PLoS ONE, 2014;9(7):e101800.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. Adipocytic cells augment the support of primitive hematopoietic cells in vitro but have no effect in the bone marrow niche under homeostatic conditions.
    Authors: Spindler, Tassja J, Tseng, Alan W, Zhou, Xiaoying, Adams, Gregor B
    Stem Cells Dev, 2014;23(4):434-41.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. Dose-dependent effect of estrogen suppresses the osteo-adipogenic transdifferentiation of osteoblasts via canonical Wnt signaling pathway.
    Authors: Gao B, Huang Q, Lin Y, Wei B, Guo Y, Sun Z, Wang L, Fan J, Zhang H, Han Y, Li X, Shi J, Liu J, Yang L, Luo Z
    PLoS ONE, 2014;9(6):e99137.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. Wnt signaling in form deprivation myopia of the mice retina.
    Authors: Ma M, Zhang Z, Du E, Zheng W, Gu Q, Xu X, Ke B
    PLoS ONE, 2014;9(4):e91086.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  15. The hippo pathway effector Yap controls patterning and differentiation of airway epithelial progenitors.
    Authors: Mahoney J, Mori M, Szymaniak A, Varelas X, Cardoso W
    Dev Cell, 2014;30(2):137-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  16. Hmga2 is required for canonical WNT signaling during lung development.
    Authors: Singh I, Mehta A, Contreras A, Boettger T, Carraro G, Wheeler M, Cabrera-Fuentes H, Bellusci S, Seeger W, Braun T, Barreto G
    BMC Biol, 2014;12(0):21.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. Recombinant Wnt3a and Wnt5a elicit macrophage cytokine production and tolerization to microbial stimulation via Toll-like receptor 4.
    Authors: Yu C, Nguyen T, Irvine K, Sweet M, Frazer I, Blumenthal A
    Eur J Immunol, 2014;44(5):1480-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Hepatocyte growth factor (Hgf) stimulates low density lipoprotein receptor-related protein (Lrp) 5/6 phosphorylation and promotes canonical Wnt signaling.
    Authors: Koraishy F, Silva C, Mason S, Wu D, Cantley L
    J Biol Chem, 2014;289(20):14341-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  19. Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel.
    Authors: Jin L, Feng T, Shih H, Zerda R, Luo A, Hsu J, Mahdavi A, Sander M, Tirrell D, Riggs A, Ku H
    Proc Natl Acad Sci U S A, 2013;110(10):3907-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. Integration of multiple signaling regulates through apoptosis the differential osteogenic potential of neural crest-derived and mesoderm-derived Osteoblasts.
    Authors: Li S, Meyer N, Quarto N, Longaker M
    PLoS ONE, 2013;8(3):e58610.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. A network of heterochronic genes including Imp1 regulates temporal changes in stem cell properties.
    Authors: Nishino, Jinsuke, Kim, Sunjung, Zhu, Yuan, Zhu, Hao, Morrison, Sean J
    Elife, 2013;2(0):e00924.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  22. Cripto-1 enhances the canonical Wnt/β-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors.
    Cell Signal, 2013;25(1):178-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Mutational analysis of sclerostin shows importance of the flexible loop and the cystine-knot for Wnt-signaling inhibition.
    Authors: Boschert V, van Dinther M, Weidauer S, van Pee K, Muth E, ten Dijke P, Mueller T
    PLoS ONE, 2013;8(11):e81710.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. A modular gain-of-function approach to generate cortical interneuron subtypes from ES cells.
    Authors: Au E, Ahmed T, Karayannis T, Biswas S, Gan L, Fishell G
    Neuron, 2013;80(5):1145-58.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  25. Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration.
    Authors: Tran, Thanh H, Shi, Xiaofeng, Zaia, Joseph, Ai, Xingbin
    J Biol Chem, 2012;287(39):32651-64.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  26. Wnt signaling in amygdala-dependent learning and memory.
    Authors: Maguschak KA, Ressler KJ
    J. Neurosci., 2011;31(37):13057-67.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  27. Coordination of epithelial branching and salivary gland lumen formation by Wnt and FGF signals.
    Authors: Patel N, Sharpe PT, Miletich I
    Dev. Biol., 2011;358(1):156-67.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  28. Distinct modes of inhibition by sclerostin on bone morphogenetic protein and Wnt signaling pathways.
    Authors: Krause C, Korchynskyi O, de Rooij K, Weidauer SE, de Gorter DJ, van Bezooijen RL, Hatsell S, Economides AN, Mueller TD, Lowik CW, ten Dijke P
    J. Biol. Chem., 2010;285(53):41614-26.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  29. Chemokine-Like Receptor 1 Is a Novel Wnt Target Gene that Regulates Mesenchymal Stem Cell Differentiation.
    Authors: Muruganandan S, Govindarajan R, McMullen N, Sinal C
    Stem Cells, 0;35(3):711-724.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 29 Citations
Average Rating: 4.5 (Based on 2 reviews)

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