Recombinant Mouse Dkk-4 Protein

Carrier Free

Catalog # Availability Size / Price Qty
3105-DK-010/CF

With Carrier

Catalog # Availability Size / Price Qty
3105-DK-010
Recombinant Mouse Dkk-4 Protein Bioactivity
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Product Details
Citations (1)
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Recombinant Mouse Dkk-4 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 1-4 µg/mL.
Optimal concentrations should be determined by each laboratory for each application. Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-4 (Catalog # 3105-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN). The ED50 for this effect is 0.2-1.2 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Dkk-4 protein
Leu19-Ile221 (Arg67Ser and Arg70Ser), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu19
Predicted Molecular Mass
23 kDa
SDS-PAGE
31 kDa, reducing conditions

Product Datasheets

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3105-DK (with carrier)

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3105-DK/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3105-DK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3105-DK/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Dkk-4 Protein Bioactivity View Larger

Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-4 (Catalog # 3105-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED50 for this effect is 0.2-1.2 µg/mL (orange circles).

Reconstitution Calculator

Reconstitution Calculator

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Background: Dkk-4

Dickkopf related protein 4 (Dkk-4) is a member of the Dickkopf protein family that includes Dkk-1, -2, -3, and -4 and a related protein, Soggy (1). Expression of Dkk-4 has only been shown with sensitive PCR techniques during early embryonic development in mice (2), in differentiated human ES cells (3), or in mice that express dominant-active beta -catenin elevating Wnt signaling in the forebrain (4). Dkk proteins are secreted proteins that are synthesized as precursors with an N-terminal signal peptide; all have two conserved cysteine-rich domains separated by a linker region which contains a potential furin type proteolytic cleavage site. The domains contain 10 cysteines each; prokineticin and colipase families show a configuration of cysteines similar to the second motif (5). Mouse Dkk-4 shows 91%, 72% and 71% amino acid (aa) identity with rat, human and canine Dkk-4, respectively, and 40-46% aa identity with other mouse Dkk proteins. Dkk-4 is predicted as a 25 kDa protein, but transfection of 293T cells produces a shorter (15-17 kDa) form containing only the second cysteine-rich domain, as well as longer (30-32 and 40 kDa) forms that do not appear to be glycosylated or form covalent multimers (1). Of the four Dkk proteins, Dkk-4 is the most like Dkk-1. Both are unequivocal antagonists of the canonical Wnt signaling pathway (1, 6), which is activated by Wnt protein engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (7). Dkk-1 and Dkk-4 antagonize Wnt by direct high-affinity binding to LRP5/6, forming ternary complexes of LRP5/6 with the Kremens protein Krm2. Internalization of the complex is triggered, making LRP5/6 unavailable for interaction with Wnt ligands (6-9).

References
  1. Krupnik, V.E. et al. (1999) Gene 238:301.
  2. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  3. Katoh, Y. and M. Katoh (2005) Int. J. Mol. Med. 16:477.
  4. Diep, D.B. et al. (2004) Dev. Brain Res. 153:261.
  5. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582.
  6. Mao, B. and C. Niehrs (2003) Gene 302:179.
  7. Logan, C.Y. and R. Nusse (2004) Ann. Rev. Cell Dev. Biol. 20:781.
  8. Mao, B. et al. (2002) Nature 417:664.
  9. Mao, B. et al. (2001) Nature 411:321.
Long Name
Dickkopf-4
Entrez Gene IDs
27121 (Human); 234130 (Mouse)
Alternate Names
dickkopf (Xenopus laevis) homolog 4; dickkopf homolog 4 (Xenopus laevis); dickkopf-4; dickkopf-related protein 4; Dkk4; Dkk-4; hDkk-4; MGC129562; MGC129563

Citation for Recombinant Mouse Dkk-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification of dkk4 as a target of Eda-A1/Edar pathway reveals an unexpected role of ectodysplasin as inhibitor of Wnt signalling in ectodermal placodes.
    Authors: Fliniaux I, Mikkola ML, Lefebvre S, Thesleff I
    Dev. Biol., 2008-04-26;320(1):60-71.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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