>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Nectin-2/CD112 (Catalog # 3869-N2) is coated at 1 µg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera
binds with a typical ED50 of 0.3-1.8 μg/mL.
Mouse myeloma cell line, NS0-derived mouse DNAM-1/CD226 protein
When Recombinant Mouse Nectin-2/ CD112 (Catalog # 3869-N2) iscoated at 1 μg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera (Catalog #4436-DN) binds with a typical ED50 of 0.3-1.8 μg/mL.
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse DNAM has a 236 amino acid (aa) extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a 58 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, mouse DNAM-1 shares 52% and 86% aa sequence identity with human and rat DNAM-1, respectively. Additional cDNA transcripts of mouse DNAM-1 may give rise to secreted or transmembrane isoforms with ECD deletions. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112 (2-4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (3-7), induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation; conversely, it inhibits the formation of osteoclasts (8-11). Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung (12). On activated, but not resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to tyrosine and serine phosphorylation of DNAM-1 during activation (7, 10, 13-15).
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Morimoto, K. et al. (2007) Oncogene July 16 [Epub ahead of print].
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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