Recombinant Mouse DNAM-1/CD226 Fc Chimera Protein, CF

(1 citations)   
  • Purity
    >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse Nectin-2/CD112 (Catalog # 3869-N2) is coated at 1 µg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera binds with a typical ED50 of 0.3-1.8 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived
    Mouse DNAM-1/CD226
    Accession # Q8K4F0
    IEGRMDP Mouse IgG2A
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
  • Predicted Molecular Mass
    53.9 kDa
    70-90 kDa, reducing conditions
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Mouse Nectin-2/
CD112 (Catalog # 3869-N2) iscoated at 1 μg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera (Catalog #4436-DN) binds with a typical ED50 of
0.3-1.8 μg/mL.
Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse DNAM has a 236 amino acid (aa) extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a 58 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, mouse DNAM-1 shares 52% and 86% aa sequence identity with human and rat DNAM-1, respectively. Additional cDNA transcripts of mouse DNAM-1 may give rise to secreted or transmembrane isoforms with ECD deletions. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112 (2-4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (3-7), induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation; conversely, it inhibits the formation of osteoclasts (8-11). Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung (12). On activated, but not resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to tyrosine and serine phosphorylation of DNAM-1 during activation (7, 10, 13-15).

  • References:
    1. Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
    2. Tahara-Hanaoka, S. et al. (2005) Biochem. Biophys. Res. Commun. 329:996.
    3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
    4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
    5. Shibuya, A. et al. (1996) Immunity 4:573.
    6. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
    7. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
    8. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
    9. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
    10. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
    11. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
    12. Morimoto, K. et al. (2007) Oncogene July 16 [Epub ahead of print].
    13. Shibuya, K. et al. (1999) Immunity 11:615.
    14. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
    15. Shibuya, A. et al. (1998) J. Immunol. 166:1671.
  • Long Name:
    DNAX Accessory Molecule 1
  • Entrez Gene IDs:
    10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
  • Alternate Names:
    CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Essential function for SAP family adaptors in the surveillance of hematopoietic cells by natural killer cells.
    Authors: Dong Z, Cruz-Munoz ME, Zhong MC, Chen R, Latour S, Veillette A
    Nat. Immunol., 2009;10(9):973-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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