Recombinant Mouse DNAM-1/CD226 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
4436-DN-050
Recombinant Mouse DNAM-1/CD226 Fc Chimera Protein, CF Data
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Recombinant Mouse DNAM-1/CD226 Fc Chimera Protein, CF Summary

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Nectin-2/CD112 (Catalog # 3869-N2) is coated at 1 µg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera binds with a typical ED50 of 0.3-1.8 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse DNAM-1/CD226 protein
Mouse DNAM-1/CD226
(Glu19-Pro254)
Accession # Q8K4F0
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Glu19
Predicted Molecular Mass
53.9 kDa
SDS-PAGE
70-90 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4436-DN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

When Recombinant Mouse Nectin-2/ CD112 (Catalog # 3869-N2) is coated at 1 μg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera (Catalog # 4436-DN) binds with a typical ED50of 0.3-1.8 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse DNAM has a 236 amino acid (aa) extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a 58 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, mouse DNAM-1 shares 52% and 86% aa sequence identity with human and rat DNAM-1, respectively. Additional cDNA transcripts of mouse DNAM-1 may give rise to secreted or transmembrane isoforms with ECD deletions. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112 (2-4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (3-7), induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation; conversely, it inhibits the formation of osteoclasts (8-11). Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung (12). On activated, but not resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to tyrosine and serine phosphorylation of DNAM-1 during activation (7, 10, 13-15).

References
  1. Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
  2. Tahara-Hanaoka, S. et al. (2005) Biochem. Biophys. Res. Commun. 329:996.
  3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  5. Shibuya, A. et al. (1996) Immunity 4:573.
  6. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
  7. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
  8. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  9. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
  10. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  11. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  12. Morimoto, K. et al. (2007) Oncogene July 16 [Epub ahead of print].
  13. Shibuya, K. et al. (1999) Immunity 11:615.
  14. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
  15. Shibuya, A. et al. (1998) J. Immunol. 166:1671.
Long Name
DNAX Accessory Molecule 1
Entrez Gene IDs
10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
Alternate Names
CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1

Citation for Recombinant Mouse DNAM-1/CD226 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Essential function for SAP family adaptors in the surveillance of hematopoietic cells by natural killer cells.
    Authors: Dong Z, Cruz-Munoz ME, Zhong MC, Chen R, Latour S, Veillette A
    Nat. Immunol., 2009;10(9):973-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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