Recombinant Mouse EphA2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
639-A2-200
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Recombinant Mouse EphA2 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind Recombinant Mouse Ephrin‑A1 Fc Chimera (Catalog # 602-A1) in a functional ELISA.
Immobilized Recombinant Mouse EphA2 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin-A1 Fc Chimera with a linear range of 0.078-5 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse EphA2 protein
Mouse EphA2
(Ala22-Ala535)
Accession # AAA82113
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
84 kDa (monomer)
SDS-PAGE
105 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

639-A2

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphA2

EphA2, also known as Eck, Myk2, and Sek2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA2 has been shown to bind ephrin-A3, ephrin-A1, ephrin-A5, ephrin-A4, and ephrin-A2 (2, 3). The extracellular domains of mouse and human EphA2 share greater than 92% amino acid identity. Only membrane-bound or Fc‑clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Entrez Gene IDs
1969 (Human); 13836 (Mouse)
Alternate Names
ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK

Citations for Recombinant Mouse EphA2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2
    Authors: M Takasugi, R Okada, A Takahashi, D Virya Chen, S Watanabe, E Hara
    Nat Commun, 2017;8(0):15729.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. ADAM12-cleaved ephrin-A1 contributes to lung metastasis.
    Authors: Ieguchi K, Tomita T, Omori T, Komatsu A, Deguchi A, Masuda J, Duffy S, Coulthard M, Boyd A, Maru Y
    Oncogene, 2015;33(17):2179-90.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. High Affinity Binders to EphA2 Isolated from Abdurin Scaffold Libraries; Characterization, Binding and Tumor Targeting.
    Authors: Ullman C, Mathonet P, Oleksy A, Diamandakis A, Tomei L, Demartis A, Nardi C, Sambucini S, Missineo A, Alt K, Hagemeyer C, Harris M, Hedt A, Weis R, Gehlsen K
    PLoS ONE, 2015;10(8):e0135278.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation.
    Authors: Giorgio C, Hassan Mohamed I, Flammini L, Barocelli E, Incerti M, Lodola A, Tognolini M
    PLoS ONE, 2011;6(3):e18128.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  5. Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling.
    Authors: Afshari FT, Kwok JC, Fawcett JW
    J. Neurosci., 2010;30(12):4246-55.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  6. EphA2 receptor mediates increased vascular permeability in lung injury due to viral infection and hypoxia.
    Authors: Cercone MA, Schroeder W, Schomberg S, Carpenter TC
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(5):L856-63.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  7. A human antibody-drug conjugate targeting EphA2 inhibits tumor growth in vivo.
    Authors: Jackson D, Gooya J, Mao S, Kinneer K, Xu L, Camara M, Fazenbaker C, Fleming R, Swamynathan S, Meyer D, Senter PD, Gao C, Wu H, Kinch M, Coats S, Kiener PA, Tice DA
    Cancer Res., 2008;68(22):9367-74.
    Species: N/A
    Sample Types: N/A
    Applications: Binding Assay
  8. Antiangiogenic and antitumor efficacy of EphA2 receptor antagonist.
    Authors: Dobrzanski P, Hunter K, Jones-Bolin S, Chang H, Robinson C, Pritchard S, Zhao H, Ruggeri B
    Cancer Res., 2004;64(3):910-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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Recombinant Mouse EphA2 Fc Chimera Protein, CF
By Anonymous on 01/29/2019
Application: Binding assay/Protein-protein interaction