Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
602-A1-200
Product Details
Citations (18)
FAQs
Supplemental Products
Reviews (1)

Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rmEphA2/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmEphrin-A1 Fc Chimera with a linear range of 0.16-10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Ephrin-A1 protein
Mouse Ephrin-A1
(Asp19 - Ser182)
Accession # P52793
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
46.8 kDa (monomer)
SDS-PAGE
50-55 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

602-A1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Ephrin-A1

Ephrin-A1, also known as B61 and LERK-1, is a member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Mouse Ephrin-A1 is synthesized with an 17 amino acid (aa) signal peptide, a 165 aa mature chain, and a 23 aa C‑terminal propeptide which is removed prior to GPI linkage of Ephrin-A1 to the membrane (3, 4). It can also be released as a soluble molecule (3, 5, 6). The mature 21 ‑ 25 kDa mouse Ephrin-A1 shares 85% and 94% aa sequence identity with human and rat Ephrin-A1, respectively. Ephrin-A1 is widely expressed on endothelial and epithelial cells, particularly in the lung, intestine, liver, and skin (4, 8). It is expressed on resting CD4+ T cells but is down‑regulated following activation (7, 8). Ligation of Ephrin-A1 on CD4+ T cells inhibits cell proliferation and activation, although soluble Ephrin-A1 can promote T cell chemotaxis (7, 8). In cancer, Ephrin‑A1 is expressed by tumor cells as well as on the tumor-associated vasculature (5, 6, 9). It inhibits tumor cell proliferation and migration but also supports tumor growth by promoting angiogenesis (10 - 12). Soluble Ephrin-A1 additionally promotes neuronal survival and neurite extension (13).

References
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Takahashi, H. and T. Ikeda (1995) Oncogene 11:879.
  4. Shao, H. et al. (1995) J. Biol. Chem. 270:5636.
  5. Easty, D.J. et al. (1995) Cancer Res. 55:2528.
  6. Cui, X.-D. et al. (2010) Int. J. Cancer 126:940.
  7. Wohlfahrt, J.G. et al. (2004) J. Immunol. 172:843.
  8. Aasheim, H.-C. et al. (2005) Blood 105:2869.
  9. Ogawa, K. et al. (2000) Oncogene 19:6043.
  10. Liu, D.-P. et al. (2007) Int. J. Oncol. 30:865.
  11. Brantley-Sieders, D.M. et al. (2006) Cancer Res. 66:10315.
  12. Pandey, A. et al. (1995) Science 268:567.
  13. Magal, E. et al. (1996) J. Neurosci. Res. 43:735.
Entrez Gene IDs
1942 (Human); 13636 (Mouse)
Alternate Names
B61; ECKLG; EFL1; EFL-1; EFNA1; EPH-related receptor tyrosine kinase ligand 1; EphrinA1; Ephrin-A1; EPLG1TNF alpha-induced protein 4; Immediate early response protein B61; LERK1; LERK1LERK-1; ligand of eph-related kinase 1; TNFAIP4B61; Tumor necrosis factor alpha-induced protein 4; tumor necrosis factor, alpha-induced protein 4

Citations for Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Protein kinase C phosphorylates the EphA2 receptor on serine 892 in the regulatory linker connecting the kinase and SAM domains
    Authors: MP Gehring, EB Pasquale
    Cell. Signal., 2020;73(0):109668.
    Species: Human
    Sample Types: Whole Cell
    Applications: Bioassay
  2. Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus
    Authors: ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu
    Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
    Species: Virus - Henipavirus
    Sample Types: Recombinant Protein
    Applications: Coprecipitation Assay
  3. ADAM12-cleaved ephrin-A1 contributes to lung metastasis.
    Authors: Ieguchi K, Tomita T, Omori T, Komatsu A, Deguchi A, Masuda J, Duffy S, Coulthard M, Boyd A, Maru Y
    Oncogene, 2015;33(17):2179-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. SLAP displays tumour suppressor functions in colorectal cancer via destabilization of the SRC substrate EPHA2.
    Authors: Naudin C, Sirvent A, Leroy C, Larive R, Simon V, Pannequin J, Bourgaux J, Pierre J, Robert B, Hollande F, Roche S
    Nat Commun, 2014;5(0):3159.
  5. Phosphorylation of cyclin-dependent kinase 5 (Cdk5) at Tyr-15 is inhibited by Cdk5 activators and does not contribute to the activation of Cdk5.
    Authors: Kobayashi H, Saito T, Sato K, Furusawa K, Hosokawa T, Tsutsumi K, Asada A, Kamada S, Ohshima T, Hisanaga S
    J Biol Chem, 2014;289(28):19627-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation.
    Authors: Giorgio C, Hassan Mohamed I, Flammini L, Barocelli E, Incerti M, Lodola A, Tognolini M
    PLoS ONE, 2011;6(3):e18128.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  7. Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis.
    Authors: Dunaway CM, Hwang Y, Lindsley CW
    Mol. Cell. Biol., 2011;31(3):404-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling.
    Authors: Afshari FT, Kwok JC, Fawcett JW
    J. Neurosci., 2010;30(12):4246-55.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. EphA2 engages Git1 to suppress Arf6 activity modulating epithelial cell-cell contacts.
    Authors: Miura K, Nam JM, Kojima C, Mochizuki N, Sabe H
    Mol. Biol. Cell, 2009;20(7):1949-59.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor.
    Authors: Wykosky J, Palma E, Gibo DM
    Oncogene, 2008;27(58):7260-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Hypoxia-inducible transcription factor-2alpha in endothelial cells regulates tumor neovascularization through activation of ephrin A1.
    Authors: Yamashita T, Ohneda K, Nagano M, Miyoshi C, Kaneko N, Miwa Y, Yamamoto M, Ohneda O, Fujii-Kuriyama Y
    J. Biol. Chem., 2008;283(27):18926-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Fibronectin type I repeat is a nonactivating ligand for EphA1 and inhibits ATF3-dependent angiogenesis.
    Authors: Masuda J, Usui R, Maru Y
    J. Biol. Chem., 2008;283(19):13148-55.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  13. Neural precursors derived from human embryonic stem cells maintain long-term proliferation without losing the potential to differentiate into all three neural lineages, including dopaminergic neurons.
    Authors: Hong S, Kang UJ, Isacson O, Kim KS
    J. Neurochem., 2007;104(2):316-24.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  14. Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth.
    Authors: Chaudhari A, Mahfouz M, Fialho AM, Yamada T, Granja AT, Zhu Y, Hashimoto W, Schlarb-Ridley B, Cho W, Das Gupta TK, Chakrabarty AM
    Biochemistry, 2007;46(7):1799-810.
    Species: Human
    Sample Types: Peptide
    Applications: Binding Assay
  15. EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets.
    Authors: Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E
    Cell, 2007;129(2):359-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. A conditional feedback loop regulates Ras activity through EphA2.
    Authors: Macrae M, Neve RM, Rodriguez-Viciana P, Haqq C, Yeh J, Chen C, Gray JW, McCormick F
    Cancer Cell, 2005;8(2):111-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Ephrin stimulation modulates T cell chemotaxis.
    Authors: Sharfe N, Freywald A, Toro A, Dadi H, Roifman C
    Eur. J. Immunol., 2002;32(12):3745-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation.
    Authors: Barquilla A, Lamberto I, Heynen-Genel S, Brill L, Pasquale E
    Mol Biol Cell, 0;27(17):2757-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF
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