Recombinant Mouse Fas Ligand/TNFSF6 Protein

Formulations:
Catalog # Availability Size / Price Qty
6128-SA-025
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Recombinant Mouse Fas Ligand/TNFSF6 Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 1-8 ng/mL in the presence of 2.5 µg/mL of a cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060).
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Fas Ligand/TNFSF6 protein
Hemagglutinin Tag 
(YPYDVPDYA)
GCN4-IZ
(GGGS)3 Mouse Fas Ligand
(Gln101-Leu279)
Accession # P41047
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Tyr
Predicted Molecular Mass
25.9 kDa  (monomer)
SDS-PAGE
40-55 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6128-SA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6128-SA/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fas Ligand/TNFSF6

Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a 40 kDa type II transmembrane member of the TNF superfamily of proteins. Its ability to induce apoptosis in target cells plays an important role in the development, homeostasis, and function of the immune system (1). Mature mouse Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 78 aa cytoplasmic domain (2). Within the ECD, mouse Fas Ligand shares 81% and 93% aa sequence identity with human and rat Fas Ligand, respectively. Alternate splicing generates a 16 kDa isoform that corresponds to the C-terminal 69 amino acids of the ECD (3). Both mouse and human Fas Ligand are active on mouse and human cells (4, 5). Fas Ligand is expressed as a nondisulfide-linked homotrimer on activated CD4+ Th1 cells, CD8+ cytotoxic T cells, and NK cells (1). Fas Ligand binding to Fas/CD95 on an adjacent cell triggers apoptosis in the Fas‑expressing cell (2, 4). Fas Ligand also binds DcR3 which is a soluble decoy receptor that interferes with Fas Ligand-induced apoptosis (6). Fas Ligand can be released from the cell surface by metalloproteinases as a 26 kDa soluble molecule which remains trimeric (7, 8). Shed Fas Ligand retains the ability to bind Fas, although its ability to trigger apoptosis is dramatically reduced (7, 8). In the absence of TGF‑ beta, however, Fas Ligand/Fas interactions instead promote neutrophil-mediated inflammatory responses (5, 9). Fas Ligand itself transmits reverse signals that costimulate the proliferation of freshly antigen-stimulated T cells (10). Fas Ligand‑induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites (11). This function is exploited by tumor cells which evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes (9, 12). In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity (13, 14).

References
  1. Lettau, M. et al. (2008) Curr. Med. Chem. 15:1684.
  2. Suda, T. et al. (1993) Cell 75:1169.
  3. Ayroldi, E. et al. (1999) Blood 94:3456.
  4. Takahashi, T. et al. (1994) Int. Immunol. 6:1567.
  5. Seino, K-I. et al. (1998) J. Immunol. 161:4484.
  6. Pitti, R.M. et al. (1998) Nature 396:699.
  7. Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
  8. Tanaka, M. et al. (1998) Nature Med. 4:31.
  9. Chen, J.-J. et al. (1998) Science 282:1714.
  10. Suzuki, I. and P.J. Fink (2000) Proc. Natl. Acad. Sci. 97:1707.
  11. Ferguson, T.A. and T.S. Griffith (2006) Immunol. Rev. 213:228.
  12. Ryan, A.E. et al. (2005) Cancer Res. 65:9817.
  13. Takahashi, T. et al. (1994) Cell 76:969.
  14. Lynch, D.H. et al. (1994) Immunity 1:131.
Entrez Gene IDs
356 (Human); 14103 (Mouse); 25385 (Rat)
Alternate Names
apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6

Citation for Recombinant Mouse Fas Ligand/TNFSF6 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Blockade of Fas signaling in breast cancer cells suppresses tumor growth and metastasis via disruption of Fas signaling-initiated cancer-related inflammation.
    Authors: Liu Q, Tan Q, Zheng Y, Chen K, Qian C, Li N, Wang Q, Cao X
    J Biol Chem, 2014;289(16):11522-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Mouse Fas Ligand/TNFSF6 Protein
By Anonymous on 12/16/2019
Application: Apoptosis assay