HA Tag Antibody Summary
Accession # ABB51961
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of N‑terminal HA-tag by Western Blot. Western blot shows Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP, 5 ng/lane). PVDF membrane was probed with 1 µg/mL of Mouse Anti-HA Tag Peptide Antibody Monoclonal Antibody (Catalog # MAB060) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for N-terminal HA-tagged Recombinant Human APRIL/ TNFSF13 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HA Tag
Product Datasheets
Citations for HA Tag Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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Patient-derived follicular lymphoma spheroids recapitulate lymph node signaling and immune profile uncovering galectin-9 as a novel immunotherapeutic target
Authors: Dobaño-López, C;Valero, JG;Araujo-Ayala, F;Nadeu, F;Gava, F;Faria, C;Norlund, M;Morin, R;Bernes-Lasserre, P;Arenas, F;Grau, M;López, C;López-Oreja, I;Serrat, N;Martínez-Farran, A;Hernández, L;Playa-Albinyana, H;Giménez, R;Beà, S;Campo, E;Lagarde, JM;López-Guillermo, A;Magnano, L;Colomer, D;Bezombes, C;Pérez-Galán, P;
Blood cancer journal
Species: Human
Sample Types: cell culture
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Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest
Authors: Acharya BR, Fang JS, Jeffery ED et al.
Life science alliance
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Development of Novel Small-Molecule Activators of Pyruvate Kinase Muscle Isozyme 2, PKM2, to Reduce Photoreceptor Apoptosis
Authors: Thomas J. Wubben, Sraboni Chaudhury, Brennan T. Watch, Jeanne A. Stuckey, Eric Weh, Roshini Fernando et al.
Pharmaceuticals (Basel)
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Small molecule activation of metabolic enzyme pyruvate kinase muscle isozyme 2, PKM2, circumvents photoreceptor apoptosis
Authors: Thomas J. Wubben, Mercy Pawar, Eric Weh, Andrew Smith, Peter Sajjakulnukit, Li Zhang et al.
Scientific Reports
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Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation
Authors: J Dai, MA Luftig
J. Immunol., 2018-01-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Trichostatin A resistance is facilitated by HIF-1 alpha acetylation in HeLa human cervical cancer cells under normoxic conditions
Authors: Jae-Wook Lee, Dong Hee Yang, Sojin Park, Hae-Kyoung Han, Jong-Wan Park, Bo Yeon Kim et al.
Oncotarget
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FAS apoptotic inhibitory molecule 2 is a stress-induced intrinsic neuroprotective factor in the retina
Authors: Mercy Pawar, Boris Busov, Aaruran Chandrasekhar, Jingyu Yao, David N Zacks, Cagri G Besirli
Cell Death & Differentiation
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Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection
Authors: AM Price, J Dai, Q Bazot, L Patel, PA Nikitin, R Djavadian, PS Winter, CA Salinas, AP Barry, KC Wood, EC Johannsen, A Letai, MJ Allday, MA Luftig
Elife, 2017-04-20;6(0):.
Species: Human
Sample Types: Recombinant Protein
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Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions.
Authors: Seifert M, Przekopowitz M, Taudien S, Lollies A, Ronge V, Drees B, Lindemann M, Hillen U, Engler H, Singer B, Kuppers R
Proc Natl Acad Sci U S A, 2015-01-26;112(6):E546-55.
Species: Human
Sample Types: Protein
Applications: Cross-linking -
Mitogen-induced B-cell proliferation activates Chk2-dependent G1/S cell cycle arrest.
Authors: Nikitin, Pavel A, Price, Alexande, McFadden, Karyn, Yan, Christop, Luftig, Micah A
PLoS ONE, 2014-01-30;9(1):e87299.
Species: Human
Sample Types: Protein
Applications: Cross-linking -
Epstein-Barr Virus Induces Adhesion Receptor CD226 (DNAM-1) Expression during Primary B-Cell Transformation into Lymphoblastoid Cell Lines
Authors: L Grossman, C Chang, J Dai, PA Nikitin, DD Jima, SS Dave, MA Luftig
mSphere, 2017-11-29;2(6):.
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This product has been used in combination with murine CD40L-HaTag protein to culture primary murine B cells.
