HA Tag Antibody Summary
Accession # ABB51961
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of N‑terminal HA-tag by Western Blot. Western blot shows Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP, 5 ng/lane). PVDF membrane was probed with 1 µg/mL of Mouse Anti-HA Tag Peptide Antibody Monoclonal Antibody (Catalog # MAB060) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for N-terminal HA-tagged Recombinant Human APRIL/ TNFSF13 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HA Tag
Citations for HA Tag Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation
Authors: J Dai, MA Luftig
J. Immunol., 2018;0(0):.
Sample Types: Whole Cells
Applications: Functional Assay
Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection
Authors: AM Price, J Dai, Q Bazot, L Patel, PA Nikitin, R Djavadian, PS Winter, CA Salinas, AP Barry, KC Wood, EC Johannsen, A Letai, MJ Allday, MA Luftig
Sample Types: Recombinant Protein
Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions.
Authors: Seifert M, Przekopowitz M, Taudien S, Lollies A, Ronge V, Drees B, Lindemann M, Hillen U, Engler H, Singer B, Kuppers R
Proc Natl Acad Sci U S A, 2015;112(6):E546-55.
Sample Types: Protein
Mitogen-induced B-cell proliferation activates Chk2-dependent G1/S cell cycle arrest.
Authors: Nikitin, Pavel A, Price, Alexande, McFadden, Karyn, Yan, Christop, Luftig, Micah A
PLoS ONE, 2014;9(1):e87299.
Sample Types: Protein
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Average Rating: 4.8 (Based on 8 Reviews)
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This product has been used in combination with murine CD40L-HaTag protein to culture primary murine B cells.