HA Tag Antibody

(3 citations)
(5 Reviews)
  
  • Specificity
    Specifically detects N-terminal HA-tagged proteins with a free N-terminus.
  • Source
    Monoclonal Mouse IgG2B Clone # 543851
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HA Tag Peptide
    Accession # ABB51961
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of N‑terminal HA-tag by Western Blot. Western blot shows Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP, 5 ng/lane). PVDF membrane was probed with 1 µg/mL of Mouse Anti-HA Tag Peptide Antibody Monoclonal Antibody (Catalog # MAB060) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for N-terminal HA-tagged Recombinant Human APRIL/
TNFSF13 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HA Tag
  • Entrez Gene IDs:
    ABB51961.1 (Viral)
  • Alternate Names:
    HA Epitope Tag; HA Tag; HA; Hemagglutinin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection
    Authors: AM Price, J Dai, Q Bazot, L Patel, PA Nikitin, R Djavadian, PS Winter, CA Salinas, AP Barry, KC Wood, EC Johannsen, A Letai, MJ Allday, MA Luftig
    Elife, 2017;6(0):.
    Species: Human
    Sample Type: Recombinant Protein
  2. Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions.
    Authors: Seifert M, Przekopowitz M, Taudien S, Lollies A, Ronge V, Drees B, Lindemann M, Hillen U, Engler H, Singer B, Kuppers R
    Proc Natl Acad Sci U S A, 2015;112(6):E546-55.
    Species: Human
    Sample Type: Protein
    Application: Cross-linking
  3. Mitogen-induced B-cell proliferation activates Chk2-dependent G1/S cell cycle arrest.
    Authors: Nikitin, Pavel A, Price, Alexande, McFadden, Karyn, Yan, Christop, Luftig, Micah A
    PLoS ONE, 2014;9(1):e87299.
    Species: Human
    Sample Type: Protein
    Application: Cross-linking
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Average Rating: 4.6 (Based on 5 reviews)

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Read what people are saying who have used HA Tag Antibody.

We have 5 reviews tested in 2 species: Human, Mouse.
We have 5 reviews tested in 3 applications: Functional Assay, Flow Cytometry, Western Blot.

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Excellent
(4)
Average
(1)
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Functional Assay
(1)
Flow
(1)
WB
(3)
All Applications
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Human
(3)
Mouse
(2)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Average
 Functional Assay Human Anonymous 12/06/2017
Show

Summary

ApplicationFunctional Assay
Sample TestedRecombinant protein
SpeciesHuman
  Excellent
 Flow Human Anonymous 08/23/2017
Show

Summary

ApplicationFlow Cytometry
Sample TestedJurkat human acute T cell leukemia cell line
SpeciesHuman
Western Blot HA Tag Antibody MAB060
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Excellent
 WB Mouse Anonymous 07/23/2017
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Image Details
Western Blot HA Tag Antibody MAB060
Western Blot: HA Tag Antibody [MAB060]

Summary

ApplicationWestern Blot
Sample Tested293-EBNA human EBV-expressing embryonic kidney cell line
SpeciesMouse
Western Blot HA Tag Antibody MAB060
Enlarge
Excellent
 WB Human Anonymous 07/21/2017
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Image Details
Western Blot HA Tag Antibody MAB060
Western Blot: HA Tag Antibody [MAB060]

Summary

ApplicationWestern Blot
Sample Tested293T human embryonic kidney cell line
SpeciesHuman
Western Blot HA Tag Antibody MAB060
Enlarge
Excellent
 WB Mouse Anonymous 07/20/2017
Show
Image Details
Western Blot HA Tag Antibody MAB060
Western Blot: HA Tag Antibody [MAB060]

Summary

ApplicationWestern Blot
Sample Tested293-EBNA human EBV-expressing embryonic kidney cell line
SpeciesMouse

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