HA Tag Antibody

Catalog # Availability Size / Price Qty
MAB060
MAB060-SP
HA Tag Antibody in Data
1 Image
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (7)

HA Tag Antibody Summary

Specificity
Specifically detects N-terminal HA-tagged proteins with a free N-terminus.
Source
Monoclonal Mouse IgG2B Clone # 543851
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HA Tag Peptide
Accession # ABB51961
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of N‑terminal HA-tag by Western Blot. View Larger

Detection of N‑terminal HA-tag by Western Blot. Western blot shows Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP, 5 ng/lane). PVDF membrane was probed with 1 µg/mL of Mouse Anti-HA Tag Peptide Antibody Monoclonal Antibody (Catalog # MAB060) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for N-terminal HA-tagged Recombinant Human APRIL/ TNFSF13 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HA Tag

Entrez Gene IDs
ABB51961.1 (Viral)
Alternate Names
HA Epitope Tag; HA Tag; HA; Hemagglutinin

Product Datasheets

Citations for HA Tag Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation
    Authors: J Dai, MA Luftig
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. Epstein-Barr virus ensures B cell survival by uniquely modulating apoptosis at early and late times after infection
    Authors: AM Price, J Dai, Q Bazot, L Patel, PA Nikitin, R Djavadian, PS Winter, CA Salinas, AP Barry, KC Wood, EC Johannsen, A Letai, MJ Allday, MA Luftig
    Elife, 2017;6(0):.
    Species: Human
    Sample Types: Recombinant Protein
  3. Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions.
    Authors: Seifert M, Przekopowitz M, Taudien S, Lollies A, Ronge V, Drees B, Lindemann M, Hillen U, Engler H, Singer B, Kuppers R
    Proc Natl Acad Sci U S A, 2015;112(6):E546-55.
    Species: Human
    Sample Types: Protein
    Applications: Cross-linking
  4. Mitogen-induced B-cell proliferation activates Chk2-dependent G1/S cell cycle arrest.
    Authors: Nikitin, Pavel A, Price, Alexande, McFadden, Karyn, Yan, Christop, Luftig, Micah A
    PLoS ONE, 2014;9(1):e87299.
    Species: Human
    Sample Types: Protein
    Applications: Cross-linking

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for HA Tag Antibody

Average Rating: 4.7 (Based on 7 Reviews)

5 Star
85.71%
4 Star
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3 Star
14.29%
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HA Tag Antibody
By Anonymous on 01/05/2021
Application: CellProlif Sample Tested: B cells Species: Mouse

HA Tag Antibody
By Anonymous on 10/22/2020
Application: Cell Culture Sample Tested: B cells Species: Mouse

This product has been used in combination with murine CD40L-HaTag protein to culture primary murine B cells.


HA Tag Antibody
By Anonymous on 12/06/2017
Application: Func Sample Tested: Recombinant protein Species: Human

HA Tag Antibody
By Anonymous on 08/23/2017
Application: Flow Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human

HA Tag Antibody
By Anonymous on 07/23/2017
Application: WB Sample Tested: 293-EBNA human EBV-expressing embryonic kidney cell line Species: Mouse

HA Tag Antibody
By Anonymous on 07/21/2017
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

HA Tag Antibody
By Anonymous on 07/20/2017
Application: WB Sample Tested: 293-EBNA human EBV-expressing embryonic kidney cell line Species: Mouse