Recombinant Mouse FGF-21 Protein, CF

(2 citations)   
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-2 μg/mL in the presence of Recombinant Mouse Klotho  beta (Catalog # 2619-KB).
  • Source
    Mouse myeloma cell line, NS0-derived Tyr30-Ser210
  • Accession #
  • N-terminal Sequence
    Analysis
    Tyr30 & Tyr49
  • Predicted Molecular Mass
    20 kDa
  • SDS-PAGE
    22-25 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8409-FG/CF
 
8409-FG
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA.
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
FGF-21 Stimulates Cell Proliferation.
Recombinant Mouse FGF-21 stimulated proliferation in NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-2 μg/mL in the presence of Recombinant Mouse Klotho  beta (Catalog # 2619-KB)
Background: FGF-21

Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family. Based on its structure, FGF-21 is further classified into a subfamily of FGFs along with FGF-19 and -23 (1). Mouse FGF-21 is a 210 amino acid (aa) polypeptide that contains a 120 aa core FGF domain and a hydrophobic N-terminus signal sequence. The signal sequence is cleaved to release the soluble 180 aa mature FGF-21 protein (2). At the amino acid sequence level, mature mouse FGF-21 is 81% and 92% identical to mature human and rat FGF-21, respectively. In comparison to other FGF subfamilies, a heparin-binding domain is uniquely absent in FGF-19 subfamily members. Lack of this domain confers endocrine function to FGF-19 members and enables them to freely diffuse within tissues and accumulate in the circulatory system (3, 4). The biological activity of FGF-21 requires binding to Klotho  beta, a co-receptor that is in complex with cell surface FGF receptors (FGF R) (5, 6). Binding of FGF-21 to Klotho  beta facilitates FGF R activation and autophosphorylation resulting in the initiation of multiple downstream signaling cascades (7, 8). FGF-21 cannot independently bind to FGF Rs, thus its effects are restricted to target tissues that express Klotho  beta. FGF-21 functions as a physiological regulator of cellular metabolism, including glucose uptake in adipocytes and cellular sensitivity to insulin. FGF-21 is basally expressed in the pancreas, thymus, and liver, as well as in adipose tissue (3, 9). Local and systemic metabolic stress has been shown to induce expression of FGF-21 in the liver, muscles, and fat (10-12). Modulation of FGF-21 expression is associated with a number of metabolic disorders, including obesity and diabetes (7, 13). FGF-21 is also involved in promoting cell survival and proliferation, modulating mesenchymal stem cell differentiation, regulating circadian rhythm, and controlling reproductive capacity during nutrient deprivation (14-18).

  • References:
    1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
    2. Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203.
    3. Adams, A.C. et al. (2013) Mol. Metab. 2:205.
    4. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
    5. Suzuki, M. et al. (2008) Mol. Endocrinol. 22:1006.
    6. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
    7. Gimeno, R.E. and D.E. Moller (2014) Trends Endocrinol. Metab. 25:303.
    8. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. U S A 104:7432.
    9. Kharitonenkov, A. et al. (2005) J. Clin. Invest. 115:1627.
    10. Luo, Y. and W.L. McKeehan (2013) Front. Endocrinol. (Lausanne) 4:194.
    11. Yang, C. et al. (2013) BMC Gastroenterol. 13:67.
    12. Keipert, S. et al. (2014) Am. J. Physiol. Endocrinol. Metab. 306:E469.
    13. Zhang, X. et al. (2008) Diabetes 57:1246.
    14. Wei, W. et al. (2012) Proc. Natl. Acad. Sci. USA 109:3143.
    15. Wei, W. et al. (2010) Cell. Metab. 11:503.
    16. Wan, Y. (2013) Int. J. Biochem. Cell. Biol. 45:546.
    17. Bookout, A.L. et al. (2013) Nat. Med. 19:1147.
    18. Owen, B.M. et al. (2013) Nat. Med. 19:1153.
  • Long Name:
    Fibroblast Growth Factor 21
  • Entrez Gene IDs:
    26291 (Human); 56636 (Mouse)
  • Alternate Names:
    FGF21; FGF-21; fibroblast growth factor 21
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Peripherally derived FGF21 promotes remyelination in the central nervous system
    Authors: M Kuroda, R Muramatsu, N Maedera, Y Koyama, M Hamaguchi, H Fujimura, M Yoshida, M Konishi, N Itoh, H Mochizuki, T Yamashita
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Chronic Over-expression of Fibroblast Growth Factor 21 Increases Bile Acid Biosynthesis by Opposing FGF15/19 Action
    EBioMedicine, 2016;0(0):.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Standard

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