Recombinant Mouse Follistatin 288 (FS-288) Protein

Carrier Free

Catalog # Availability Size / Price Qty
769-FS-025/CF

With Carrier

Catalog # Availability Size / Price Qty
769-FS-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (10)
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Recombinant Mouse Follistatin 288 (FS-288) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.1‑0.4 µg/mL in the presence of 7.5 ng/mL rhActivin A.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Follistatin protein
Gly30-Asn317, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
31 kDa
SDS-PAGE
35-40 kDa, reducing conditions

Product Datasheets

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769-FS (with carrier)

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769-FS/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

769-FS

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

769-FS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Follistatin

Follistatin (FS) was initially identified as a follicle-stimulating hormone inhibiting substance found in ovarian follicular fluid. It has since been shown that FS is a high-affinity activin-binding protein that can act as an activin antagonist. Two alternatively spliced follistatin mRNAs, encoding mature FS with 288 amino acid (aa) residues (FS-288) and 315 aa residues (FS-315), exist. Natural FS purified from porcine ovaries is primarily a carboxy-terminal truncated form of FS-315 composed of 300 aa residues. FS-288 binds with high-affinity to cell-surface heparan sulfate proteoglycans whereas FS-315 binds with low-affinity. Cell surface-associated FS has been suggested to play a role in the clearance and bioavailability of activin in vivo. Besides activin, FS has also been shown to bind with multiple BMPs and to inhibit BMP activity in early Xenopus embryos. FS deficient mice have been shown to have multiple embryonic defects that will result in death shortly after birth. Overexpression of FS can also cause reproductive defects in transgenic mice.

References
  1. Iemura, S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:9337.
  2. Guo, Q. (1998) Mol. Endocrinol. 12:96.
  3. Hashimoto, O. et al. (1997) J. Biol. Chem. 272:13835.
Entrez Gene IDs
10468 (Human); 14313 (Mouse)
Alternate Names
follistatin isoform FST317; Follistatin; FS; FSActivin-binding protein; FST

Citations for Recombinant Mouse Follistatin 288 (FS-288) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Liver fibrosis-induced muscle atrophy is mediated by elevated levels of circulating TNF&alpha
    Authors: T Kurosawa, M Goto, N Kaji, S Aikiyo, T Mihara, M Ikemoto-Ue, M Toyoda, N Kanazawa, T Nakazawa, M Hori, A Uezumi
    Cell Death & Disease, 2021-01-07;12(1):11.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f+ cells
    Authors: A Ikeda, T Yamamoto, J Mineshiba, S Takashiba
    Sci Rep, 2020-11-17;10(1):19959.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Bone morphogenetic protein 7 promotes resistance to immunotherapy
    Authors: MA Cortez, F Masrorpour, C Ivan, J Zhang, A Younes, Y Lu, MR Estecio, HB Barsoumian, H Menon, MDS Caetano, R Ramapriyan, JE Schoenhals, X Wang, F Skoulidis, MD Wasley, G Calin, P Hwu, JW Welsh
    Nat Commun, 2020-09-24;11(1):4840.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Follistatin288 Regulates Germ Cell Cyst Breakdown and Primordial Follicle Assembly in the Mouse Ovary.
    Authors: Wang Z, Niu W, Wang Y, Teng Z, Wen J, Xia G, Wang C
    PLoS ONE, 2015-06-15;10(6):e0129643.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  5. Regenerative hair waves in aging mice and extra-follicular modulators follistatin, dkk1, and sfrp4.
    Authors: Chen C, Murray P, Jiang T, Plikus M, Chang Y, Lee O, Widelitz R, Chuong C
    J Invest Dermatol, 2014-03-11;134(8):2086-96.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Signals from the lens and Foxc1 regulate the expression of key genes during the onset of corneal endothelial development.
    Authors: Silla Z, Naidoo J, Kidson S, Sommer P
    Exp Cell Res, 2014-01-25;322(2):381-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Follistatin promotes adipocyte differentiation, browning, and energy metabolism.
    Authors: Braga M, Reddy S, Vergnes L, Pervin S, Grijalva V, Stout D, David J, Li X, Tomasian V, Reid C, Norris K, Devaskar S, Reue K, Singh R
    J Lipid Res, 2014-01-17;55(3):375-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells.
    Authors: Roelandt P, Pauwelyn KA, Sancho-Bru P
    PLoS ONE, 2010-08-11;5(8):e12101.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal myogenesis.
    Authors: Sun Y, Ge Y, Drnevich J, Zhao Y, Band M, Chen J
    J. Cell Biol., 2010-06-21;189(7):1157-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm.
    Authors: Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM
    Development, 2006-08-30;133(19):3787-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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