Recombinant Mouse GDF-3 Protein, CF

Newer Version Available: 9009-GD
NEW
A New rh GDF-3 Protein is Now Available! Enhanced activity induces phosphorylation of SMAD-2; Mammalian cell expressed!
Catalog # Availability Size / Price Qty
958-G3-010
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Recombinant Mouse GDF-3 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind with Recombinant Human Activin RIB/ALK‑4 Fc Chimera (Catalog # 808-AR) in the presence of Recombinant Mouse Cripto (Catalog # 1538-CR) in functional ELISA. Chen, et al. (2006) Development 133:319. Immobilized Recombinant Mouse (rm) GDF-3 at 1 µg/mL (100 µL/well) can bind Recombinant Human Activin RIB/ALK‑4 Fc Chimera (Catalog # 808-AR) with an apparent Kd <100 nM.
The activity of rmGDF-3 is not fully tested in cell-based assays. Please contact Technical Service for product related questions.
Source
E. coli-derived mouse GDF-3 protein
Ala253-Gly366
Accession #
N-terminal Sequence
Analysis
Ala253
Predicted Molecular Mass
13.1 kDa (monomer)

Product Datasheets

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958-G3

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

958-G3

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCI.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: GDF-3

GDF-3 (previously called Vgr-2) is a TGF-beta superfamily member belonging to the growth/differentiation factor family (1, 2). GDF-3 is expressed in undifferentiated embryonic stem (ES) cells, white adipose tissue and the brain (2-4). The 366 amino acid (aa) mouse GDF-3 contains a 22 aa signal sequence, a 230 aa propeptide and a 114 aa mature protein that contains one potential N-glycosylation site. The mature region contains a cysteine-knot structure that is conserved throughout family members. However, it lacks the fourth cysteine which is responsible for the formation of an inter-molecular disulfide bond, so GDF-3 may exist as a non-covalent homodimer (2, 5). Mature mouse GDF-3 shares 90% and 83% aa sequence identity with rat and human GDF-3, respectively. Most of GDF-3 is present as the uncleaved prepro form (6). The uncleaved and the mature forms both appear to have activity, but that activity may differ (5-8). All forms can oppose BMPs. In ES cells, inhibition of BMP2 signaling by GDF-3 maintains pluripotency (5, 7). GDF-3 also influences early cell fate decisions; for example, deletion of mouse GDF-3 produces defects in the anterior visceral endoderm of the pre-gastrulation embryo (6-8). GDF-3 cooperates with GDF-1 in embryogenesis, and the mature protein has nodal-like activity (8, 9). Although GDF family members signal through BMP receptors (ALK1, 2, 3 and 6), which activate Smads 1, 5 and 8, GDF-3 signaling through ALK4 and ALK7, which activate Smads 2 and 3, has also been reported (9, 10). In adipocytes, GDF-3 is induced by a high fat diet, promoting adipogenesis and obesity (3, 10, 11).

References
  1. Levine, A.J. and A.H. Brivanlou (2006) Cell Cycle 5:1069.
  2. McPherron, A.C. and S.-J. Lee (1993) J. Biol. Chem. 268:3444. Mouse cloning
  3. Wang, W. et al. (2004) Biochem. Biophys. Res. Comm. 321:1024.
  4. Hexige, S. et al. (2005) Neurosci. Lett. 389:83.
  5. Levine, A.J. et al. (2009) Dev. Biol. 325:43.
  6. Levine, A.J. and A.H. Brivanlou (2005) Development 133:209.
  7. Peerani, R. et al. (2007) EMBO J. 26:4744.
  8. Chen, C. et al. (2006) Development 133:319.
  9. Andersson, O. et al. (2007) Dev. Biol. 311:500.
  10. Andersson, O. et al. (2008) Proc. Natl. Acad. Sci. USA 105:7252.
  11. Shen, J.J. et al. (2009) Mol. Endocrinol. 23:113.
Long Name
Growth Differentiation Factor 3
Entrez Gene IDs
9573 (Human); 14562 (Mouse)
Alternate Names
GDF3; GDF-3; growth differentiation factor 3; growth/differentiation factor 3; KFS3; MCOP7; MCOPCB6; Vgr-2

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