Recombinant Mouse GDF-5 Protein, CF

(6 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.2‑1.2 µg/mL.
  • Source
    E. coli-derived Ala376-Arg495, with and without an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met & Ala376
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    13.7 kDa (monomer)
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
853-G5/CF
 
853-G5
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 150 μg/mL in sterile 4 mM HCl.
Reconstitution Reconstitute at 150 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDF-5/BMP-14

Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). GDF-5, -6, and -7 are a defined subgroup of the BMP family (3). GDF-­5 is synthesized as a large precursor protein that consists of an N­-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature mouse GDF-5 shares 99% aa sequence identity with both mature human and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (4, 5). Similar to other BMP family proteins, GDF-5 signaling is antagonized by Noggin (6). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis (7-11). Exogenous GDF-5 has been reported to promote chondrogenesis, osteogenesis, and angiogenesis in mesenchymal stem cells in vivo and in vitro (12-14). Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis (15-18).

  • References:
    1. Storm, E.E. et al. (1994) Nature 368:639.
    2. Hotten, G. et al. (1994) Biochem. Biophys. Res. Commun. 204:646.
    3. Mikic, B. (2004) Ann. Biomed. Eng. 32:466.
    4. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
    5. Mueller, T.D. and J. Nickel (2012) FEBS Lett. 586:1846.
    6. Bragdon, B. et al. (2011) Cell. Signal. 23:609.
    7. Sieber, C. et al. (2006) Biol. Chem. 387:451.
    8. Osorio, C. et al. (2013) Development 140:4751.
    9. Wood, T.K. et al. (2005) J. Neurosci. Res. 80:759.
    10. Francis-West, P.H. et al. (1999) Development 126:1305.
    11. Brunet, L.J. et al. (1998) Science 280:1455.
    12. Zeng, Q. et al. (2007) Bone 40:374.
    13. Coleman, C.M. et al. (2013) Stem Cells Dev. 22:1968.
    14. Cheng, X. et al. (2012) Cells Tissues Organs 196:56.
    15. Zhang, Y. et al. (2014) Exp. Mol. Med. 46:e79.
    16. Miyamoto, Y. et al. (2007) Nat. Genet. 39:529.
    17. Vaes, R.B. et al. (2009) Ann. Rheum. Dis. 68:1754.
    18. Degenkolbe, E. et al. (2013) PLoS Genet. 9:e1003846.
  • Long Name:
    Growth Differentiation Factor 5
  • Entrez Gene IDs:
    8200 (Human); 14563 (Mouse)
  • Alternate Names:
    BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Generation of articular chondrocytes from human pluripotent stem cells.
    Authors: Craft A, Rockel J, Nartiss Y, Kandel R, Alman B, Keller G
    Nat Biotechnol, 2015;33(6):638-45.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Human chondrocytes respond discordantly to the protein encoded by the osteoarthritis susceptibility gene GDF5.
    Authors: Ratnayake M, Ploger F, Santibanez-Koref M, Loughlin J
    PLoS ONE, 2014;9(1):e86590.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Growth differentiation factor-5 promotes brown adipogenesis in systemic energy expenditure.
    Authors: Hinoi E, Nakamura Y, Takada S, Fujita H, Iezaki T, Hashizume S, Takahashi S, Odaka Y, Watanabe T, Yoneda Y
    Diabetes, 2013;63(1):162-75.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Growth differentiation factor 5 regulates cardiac repair after myocardial infarction.
    Authors: Zaidi SH, Huang Q, Momen A, Riazi A, Husain M
    J. Am. Coll. Cardiol., 2010;55(2):135-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
    Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
    J. Biol. Chem., 2008;283(20):13874-88.
    Species: Human
    Sample Type: Recombinant Protein
    Application: complex formation
  6. Recombinant growth/differentiation factor-5 (GDF-5) stimulates osteogenic differentiation of marrow mesenchymal stem cells in porous hydroxyapatite ceramic.
    Authors: Shimaoka H, Dohi Y, Ohgushi H, Ikeuchi M, Okamoto M, Kudo A, Kirita T, Yonemasu K
    J Biomed Mater Res A, 2004;68(1):168-76.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo

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