Recombinant Mouse GDF-5 Protein

Formulations:
Catalog # Availability Size / Price Qty
853-G5-050
Product Details
Citations (8)
FAQs
Reviews

Recombinant Mouse GDF-5 Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.2‑1.2 µg/mL.
Source
E. coli-derived mouse GDF-5/BMP-14 protein
Ala376-Arg495, with and without an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met and Ala376
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
13.7 kDa (monomer)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

853-G5

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 150 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

853-G5/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 150 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-5/BMP-14

Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). GDF-5, -6, and -7 are a defined subgroup of the BMP family (3). GDF-­5 is synthesized as a large precursor protein that consists of an N­-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature mouse GDF-5 shares 99% aa sequence identity with both mature human and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (4, 5). Similar to other BMP family proteins, GDF-5 signaling is antagonized by Noggin (6). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis (7-11). Exogenous GDF-5 has been reported to promote chondrogenesis, osteogenesis, and angiogenesis in mesenchymal stem cells in vivo and in vitro (12-14). Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis (15-18).

References
  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Hotten, G. et al. (1994) Biochem. Biophys. Res. Commun. 204:646.
  3. Mikic, B. (2004) Ann. Biomed. Eng. 32:466.
  4. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  5. Mueller, T.D. and J. Nickel (2012) FEBS Lett. 586:1846.
  6. Bragdon, B. et al. (2011) Cell. Signal. 23:609.
  7. Sieber, C. et al. (2006) Biol. Chem. 387:451.
  8. Osorio, C. et al. (2013) Development 140:4751.
  9. Wood, T.K. et al. (2005) J. Neurosci. Res. 80:759.
  10. Francis-West, P.H. et al. (1999) Development 126:1305.
  11. Brunet, L.J. et al. (1998) Science 280:1455.
  12. Zeng, Q. et al. (2007) Bone 40:374.
  13. Coleman, C.M. et al. (2013) Stem Cells Dev. 22:1968.
  14. Cheng, X. et al. (2012) Cells Tissues Organs 196:56.
  15. Zhang, Y. et al. (2014) Exp. Mol. Med. 46:e79.
  16. Miyamoto, Y. et al. (2007) Nat. Genet. 39:529.
  17. Vaes, R.B. et al. (2009) Ann. Rheum. Dis. 68:1754.
  18. Degenkolbe, E. et al. (2013) PLoS Genet. 9:e1003846.
Long Name
Growth Differentiation Factor 5
Entrez Gene IDs
8200 (Human); 14563 (Mouse)
Alternate Names
BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2

Citations for Recombinant Mouse GDF-5 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Signaling by growth/differentiation factor 5 through the bone morphogenetic protein receptor type IB protects neurons against kainic acid-induced neurodegeneration
    Authors: Y Zhao, M Zhang, H Liu, J Wang
    Neurosci. Lett., 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. GDF5 Mediated Enhancement of Chondrocyte Phenotype is Inhibited by Heparin; Implication for the use of Heparin in the Clinic and in Tissue Engineering Applications
    Authors: Bethanie Imogen Ayerst
    Tissue Eng Part A, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  3. Generation of articular chondrocytes from human pluripotent stem cells.
    Authors: Craft A, Rockel J, Nartiss Y, Kandel R, Alman B, Keller G
    Nat Biotechnol, 2015;33(6):638-45.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Human chondrocytes respond discordantly to the protein encoded by the osteoarthritis susceptibility gene GDF5.
    Authors: Ratnayake M, Ploger F, Santibanez-Koref M, Loughlin J
    PLoS ONE, 2014;9(1):e86590.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Growth differentiation factor-5 promotes brown adipogenesis in systemic energy expenditure.
    Authors: Hinoi E, Nakamura Y, Takada S, Fujita H, Iezaki T, Hashizume S, Takahashi S, Odaka Y, Watanabe T, Yoneda Y
    Diabetes, 2013;63(1):162-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Growth differentiation factor 5 regulates cardiac repair after myocardial infarction.
    Authors: Zaidi SH, Huang Q, Momen A, Riazi A, Husain M
    J. Am. Coll. Cardiol., 2010;55(2):135-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
    Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
    J. Biol. Chem., 2008;283(20):13874-88.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: complex formation
  8. Recombinant growth/differentiation factor-5 (GDF-5) stimulates osteogenic differentiation of marrow mesenchymal stem cells in porous hydroxyapatite ceramic.
    Authors: Shimaoka H, Dohi Y, Ohgushi H, Ikeuchi M, Okamoto M, Kudo A, Kirita T, Yonemasu K
    J Biomed Mater Res A, 2004;68(1):168-76.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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