Recombinant Mouse GFR alpha-1 His-tag Protein, CF

R&D Systems | Catalog # 10070-GR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse GFR alpha-1 His-tag Protein (10070-GR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P97785

Structure / Form

Monomer

Applications

Bioactivity
View all GFR alpha-1/GDNF R alpha-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse GFR alpha-1/GDNF R alpha-1 protein
Asp25-Ser430, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala19

Predicted Molecular Mass

47 kDa

SDS-PAGE

57-67 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat GDNF (Catalog # 512-GF) is coated at 25 ng/mL (100 μL/well), the concentration of Recombinant Mouse GFR alpha ‑1/GDNF R alpha ‑1 that produces 50% optimal binding response is 0.04-0.24 μg/mL.

Scientific Data Images for Recombinant Mouse GFR alpha-1 His-tag Protein, CF

Recombinant Mouse GFR alpha-1 His-tag Protein Binding Activity

Recombinant Mouse GFR alpha-1 His-tag Protein Binding Activity

When Recombinant Rat GDNF (Catalog # 512-GF) is coated at 25 ng/mL (100 µL/well), Recombinant Mouse GFRa-1/GDNF Ra-1 (Catalog # 10070-GR) binds with an ED50 of 0.04-0.24 µg/mL.
Recombinant Mouse GFR alpha-1 His-tag Protein SDS-PAGE

Recombinant Mouse GFR alpha-1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse GFR alpha ‑1/GDNF R alpha ‑1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57 - 67 kDa and 43 - 57 kDa, respectively.

Formulation, Preparation, and Storage

10070-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFR alpha-1/GDNF R alpha-1

Mouse GFR alpha -1/GDNF R alpha -1 contains a 24 amino acid (aa) signal peptide, a 406 aa GDNF receptor domain, and a 38 aa propeptide. Like other GPI-linked proteins, mouse GFR alpha -1/GDNF R alpha -1 has a C‑terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Mouse homolog's GDNF receptor domain contains 99% sequence identity with its rat homolog, and 95% sequence identity with its human homolog. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors (1, 2). GFR alpha -1/GDNF R alpha -1 is expressed by neuronal cells, Schwann cells, and injured sciatic nerve where it recruits c-Ret to the lipid rafts (4). It is also over-expressed in a subset of estrogen responsive breast cancers, where it enhances proliferation and cell scattering in response to GDNF (5). GDNF/GFR alpha -1/c-Ret signaling pathway is part of an inflammatory response in breast cancer. TNF alpha and IL-1 beta enhance expression of GDNF in breast cancer tissue (5). Recent studies have discovered that GFR alpha -1/GDNF R alpha -1 expression is non-existent in Alzheimer brain neurons, which leads to no axonal regrowth and apoptosis (6). Addition of exogenous GFR alpha -1/GDNF R alpha -1 to the Alzheimer brain neurons was able to restore survival and axonal regrowth (6).

References

  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:5801.
  4. Paratcha, G. et al. (2001) Neuron 29:171.
  5. Esseghir, S. et al. (2007) Cancer Res. 67:11732.
  6. Konishi, Y. et al. (2014) J Neurosci. 34:13127.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 1

Alternate Names

GDNF R alpha-1, GFR alpha1, GFRa-1, GFRA1

Entrez Gene IDs

2674 (Human); 14585 (Mouse); 25454 (Rat)

Gene Symbol

GFRA1

UniProt

P97785

Additional GFR alpha-1/GDNF R alpha-1 Products

Product Documents for Recombinant Mouse GFR alpha-1 His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse GFR alpha-1 His-tag Protein, CF

For research use only

Related Research Areas

GDNF Family

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