Recombinant Mouse GFR alpha-1 His-tag Protein, CF Summary
Asp25-Ser430, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Rat GDNF (Catalog # 512-GF) is coated at 25 ng/mL (100 μL/well), Recombinant Mouse GFR alpha ‑1/GDNF R alpha ‑1 (Catalog # 10070-GR) binds with an ED50 of 0.04‑0.24 μg/mL.
2 μg/lane of Recombinant Mouse GFR alpha ‑1/GDNF R alpha ‑1 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 57 - 67 kDa and 43 - 57 kDa, respectively.
Background: GFR alpha-1/GDNF R alpha-1
Mouse GFR alpha -1/GDNF R alpha -1 contains a 24 amino acid (aa) signal peptide, a 406 aa GDNF receptor domain, and a 38 aa propeptide. Like other GPI-linked proteins, mouse GFR alpha -1/GDNF R alpha -1 has a C‑terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Mouse homolog's GDNF receptor domain contains 99% sequence identity with its rat homolog, and 95% sequence identity with its human homolog. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors (1, 2). GFR alpha -1/GDNF R alpha -1 is expressed by neuronal cells, Schwann cells, and injured sciatic nerve where it recruits c-Ret to the lipid rafts (4). It is also over-expressed in a subset of estrogen responsive breast cancers, where it enhances proliferation and cell scattering in response to GDNF (5). GDNF/GFR alpha -1/c-Ret signaling pathway is part of an inflammatory response in breast cancer. TNF alpha and IL-1 beta enhance expression of GDNF in breast cancer tissue (5). Recent studies have discovered that GFR alpha -1/GDNF R alpha -1 expression is non-existent in Alzheimer brain neurons, which leads to no axonal regrowth and apoptosis (6). Addition of exogenous GFR alpha -1/GDNF R alpha -1 to the Alzheimer brain neurons was able to restore survival and axonal regrowth (6).
- Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
- Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
- Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:5801.
- Paratcha, G. et al. (2001) Neuron 29:171.
- Esseghir, S. et al. (2007) Cancer Res. 67:11732.
- Konishi, Y. et al. (2014) J Neurosci. 34:13127.
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