Recombinant Mouse ICOS Fc Chimera Protein, CF

R&D Systems | Catalog # 168-CS

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Mouse ICOS Fc Chimera Protein (168-CS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

NP_059508

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity
View all ICOS Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse ICOS protein
Mouse ICOS
(Glu21-Leu142)
Accession # NP_059508		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

44 kDa (monomer)

SDS-PAGE

47 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rmICOS/Fc Chimera at 1 µg/mL (100 µL/well) can bind biotinylated rhB7-H2/Fc Chimera with a linear range of 0.156-10 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

168-CS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICOS

Mouse ICOS (inducible co-stimulator), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Mouse ICOS is a homodimeric type I transmembrane protein consisting of 200 amino acids (aa) with a putative 20 aa signal sequence, a 122 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD 28 and CTLA-4. Mouse and human ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24 - 48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

References

  1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  2. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  3. Yoshinaga, S.K. et al. (1999) Nature 402:827.
  4. Hutloff, A. et al. (1999) Nature 397:263.
  5. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  6. Coyle, A.J. et al. (2000) Immunity 13:95.
  7. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.

Long Name

Inducible Co-stimulator

Alternate Names

AILIM, CD278, CRP-1

Entrez Gene IDs

29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)

Gene Symbol

ICOS

UniProt

NP_059508

Additional ICOS Products

Product Documents for Recombinant Mouse ICOS Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse ICOS Fc Chimera Protein, CF

For research use only

Citations for Recombinant Mouse ICOS Fc Chimera Protein, CF

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