Recombinant Mouse IFN-alpha 2/IFNA2 Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse IFN-alpha 2/IFNA2 (Catalog # 10149-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 20-200 pg/mL.
2 μg/lane of Recombinant Mouse IFN-alpha 2/IFNA2 (Catalog # 10149-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa.
Background: IFN-alpha 2/IFNA2
Interferon-alpha 2 (IFN alpha -2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons (1). The members of the IFN-alpha class, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1, 3, 4). IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (3). As the first highly active IFN to be cloned and produced, IFN alpha -2 has become the prototypic IFN for academic and pharmaceutical research (2). The mature extracellular domain (ECD) of mouse IFN alpha -2 shares 60% and 83% aa sequence identity with human and rat, respectively. Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3) (5). IFN alpha -2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity (6).
- Pestka, S. (2007) J Biol Chem. 282:20047.
- Paul, F. et al. (2015) Gene. 567(2):132.
- Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
- Pesch, V. et al. (2004). Journal of Virology, 78:8219.
- Wang, Y.X. et al. (2007) Am J Physiol Heart Circ Physiol. 293:H69.
- Schlaepfer, E. et al. (2019) Am Soc for Microbiology 4:e00637.
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