Recombinant Mouse IFN-kappa Protein, CF

  
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is 0.2-1.2 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Leu22-Lys199
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu22
  • Predicted Molecular Mass
    21 kDa
  • SDS-PAGE
    19-22 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8437-MK/CF
 
8437-MK
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and EDTA.
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Reconstitution Reconstitute at 200 μg/mL in sterile water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse IFN-kappa (Catalog # 8437-MK/CF) exhibits anti-viral activity in L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1.2 ng/mL.
Background: IFN-kappa
Interferon (IFN)-kappa is a member of the type I IFN family, which also includes IFN-alpha, -beta, -epsilon, and -omega. Mouse IFN-kappa is expressed at low levels in peritoneal macrophages and its expression is up-regulated by double-stranded (ds) RNA and IFN-gamma (1). Mice over-expressing IFN-kappa in pancreatic beta  cells developed type I diabetes, similar to what has been reported for mice over-expressing IFN-alpha, -beta, and -gamma (1-4). Mouse IFN-kappa shares 68% and 30% amino acid sequence identity with rat and human IFN-kappa, respectively. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity (5-7). Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression (8-11).
  • References:
    1. Vassileva, G. et al. (2003) J. Immunol. 170:5748.
    2. Stewart, T.A. et al. (1993) Science 260:1942.
    3. Sarvetnick, N. et al. (1988) Cell 52:773.
    4. Pelegrin, M. et al. (1998) J. Biol. Chem. 273:12332.
    5. LaFleur, D.W. et al. (2001) J. Biol. Chem. 276:39765.
    6. Nardelli, B. et al. (2002) J. Immunol. 169:4822.
    7. Buontempo, P.J. et al. (2006) J. Interferon Cytokine Res. 26:40.
    8. Rincon-Orozco, B. et al. (2009) Cancer Res. 69:8718.
    9. DeCarlo, C.A. et al. (2010) Lab. Invest. 90:1482.
    10. Reiser, J. et al. (2011) J. Virol. 85:11372.
    11. Sunthamala, N. et al. (2014) PLoS One 9:e91473.
  • Long Name:
    Interferon kappa
  • Entrez Gene IDs:
    56832 (Human); 387510 (Mouse)
  • Alternate Names:
    IFNK; IFNkappa; IFN-kappa
Related Research Areas

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