Recombinant Mouse IGF-II/IGF2 Protein, CF

R&D Systems | Catalog # 792-MG

R&D Systems
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse IGF-II/IGF2 Protein (792-MG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P09535

Applications

Bioactivity
View all IGF-II/IGF2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse IGF-II/IGF2 protein
Ala25-Glu91

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25

Predicted Molecular Mass

7.4 kDa

Activity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is 2-10 ng/mL.

Reviewed Applications

Read 3 reviews rated 4.7 using 792-MG in the following applications:

Formulation, Preparation, and Storage

792-MG
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGF-II/IGF2

IGF-II (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) is a secreted 8 kDa polypeptide that belongs to the insulin family of peptide growth factors (1, 2, 3). It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation (4). In human, IGF-II is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF-II levels drop after the perinatal period, an effect attributed to the lack of a key gene promoter (2, 5). This may indicate that postnatally, IGF-II has either a limited, or local effect only in rodent. For example, evidence suggests IGF-II may be the intermediary for SHH induction of VEGF attendant with local neovascularization (6). Rodent cells known to express IGF-II include astrocytes (7), hepatocytes (8), osteoblasts (9), embryonic striated muscle cells (10, 11) plus Kupffer cells and Ito cells (12). Mouse IGF-II is synthesized as a 180 amino acid (aa) preproprecursor (13). It contains a 24 aa signal sequence, a 67 aa mature region, and an 89 aa C-terminal prodomain that is alternatively referred to as the E-peptide. Mature IGF-II is 91% and 97% aa identical to human and rat IGF-II, respectively. Proper processing of IGF-II requires the chaperone activity of GRP94 (14). This generates an 8 kDa mature form, an 18 kDa, 156 aa proform, and a potential 11 kDa, 88 aa “Big” form (aa 25-112).  This 11 kDa ”Big” form would be equivalent to human 15-16 kDa IGF-II, with the 5 kDa difference attributable to the presence of O-linked glycosylation (15).  There is an additional 34 aa proteolytic fragment that is termed preptin and contains aa 93-126 of the preproprecursor.  This is distinct from IGF-II, is secreted by pancreatic b cells, and facilitates insulin secretion (16, 17). IGF-II has multiple binding partners.  It binds to IGF-IR, the Insulin receptor (IR)-type A and IGF-IR:IR-A hybrids, the type 2 IGF receptor (IGF-2R), and IGF binding proteins 1-6 (18, 19).  The first three receptors initiate downstream signaling events, the IGF-2R sequesters local IGF-II, and the six IGFBPs regulate IGF-II activity in various tissues.

References

  1. LeRoith, D. & C.T. Roberts Jr. (2003) Cancer Lett. 195:127.
  2. Werner, H. & D. LeRoith (2000) Cell. Mol. Life Sci. 57:932.
  3. Pavelic, J. et al. (2007) Indian J. Med. Res. 125:511.
  4. Varela-Nieto, I. et al. (2007) Curr. Pharm. Des. 13:687.
  5. Rotwein, P. & L.J. Hall (1990) DNA Cell Biol. 10:725.
  6. Chao, W. & P. A. D-Amore (2008) Cytokine Growth Factor Rev. 19:111.
  7. Rotwein, P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:265.
  8. Goya, L. et al. (1999) J. Biol. Chem. 274:24633.
  9. McCarthy, T.L. et al. (1992) Endocrinology 130:1303.
  10. Zindy, F. et al. (1992) J. Hepatol. 14:30.
  11. Holthuizen, P.E. et al. (1993) Regul. Pept. 48:77.
  12. Merrick, D. et al. (2007) BMC Dev. Biol. 7:65.
  13. Stempien, M.M. et al. (1986) DNA 5:357.
  14. Ostrovsky, O. et al. (2009) Mol. Biol. Cell 20:1855.
  15. Daughaday, W.H. et al. (1993) Proc. Natl. Acad. Sci. USA 90:5823.
  16. Buchanan, C.M. et al. (2001) Biochem. J. 360:431.
  17. Cornish, J. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292:E117.
  18. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
  19. Belfiore, A. (2007) Curr. Pharm. Des. 13:671.

Long Name

Insulin-like Growth Factor II

Alternate Names

C11orf43, GRDF, IGF-2, IGF2, IGFII, MSA, PEG2, PP9974

Entrez Gene IDs

3481 (Human); 16002 (Mouse)

Gene Symbol

IGF2

UniProt

P09535

Additional IGF-II/IGF2 Products

Product Documents for Recombinant Mouse IGF-II/IGF2 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IGF-II/IGF2 Protein, CF

For research use only

Citations for Recombinant Mouse IGF-II/IGF2 Protein, CF

Customer Reviews for Recombinant Mouse IGF-II/IGF2 Protein, CF (3)

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