Recombinant Mouse IGF-II/IGF2 Protein, CF

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Recombinant Mouse IGF-II/IGF2 Protein, CF Summary

Product Specifications

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 2-10 ng/mL.
E. coli-derived mouse IGF-II/IGF2 protein
Accession #
N-terminal Sequence
Predicted Molecular Mass
7.4 kDa

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IGF-II/IGF2

IGF-II (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) is a secreted 8 kDa polypeptide that belongs to the insulin family of peptide growth factors (1, 2, 3). It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation (4). In human, IGF-II is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF-II levels drop after the perinatal period, an effect attributed to the lack of a key gene promoter (2, 5). This may indicate that postnatally, IGF-II has either a limited, or local effect only in rodent. For example, evidence suggests IGF-II may be the intermediary for SHH induction of VEGF attendant with local neovascularization (6). Rodent cells known to express IGF-II include astrocytes (7), hepatocytes (8), osteoblasts (9), embryonic striated muscle cells (10, 11) plus Kupffer cells and Ito cells (12). Mouse IGF-II is synthesized as a 180 amino acid (aa) preproprecursor (13). It contains a 24 aa signal sequence, a 67 aa mature region, and an 89 aa C-terminal prodomain that is alternatively referred to as the E-peptide. Mature IGF-II is 91% and 97% aa identical to human and rat IGF-II, respectively. Proper processing of IGF-II requires the chaperone activity of GRP94 (14). This generates an 8 kDa mature form, an 18 kDa, 156 aa proform, and a potential 11 kDa, 88 aa “Big” form (aa 25-112).  This 11 kDa ”Big” form would be equivalent to human 15-16 kDa IGF-II, with the 5 kDa difference attributable to the presence of O-linked glycosylation (15).  There is an additional 34 aa proteolytic fragment that is termed preptin and contains aa 93-126 of the preproprecursor.  This is distinct from IGF-II, is secreted by pancreatic b cells, and facilitates insulin secretion (16, 17). IGF-II has multiple binding partners.  It binds to IGF-IR, the Insulin receptor (IR)-type A and IGF-IR:IR-A hybrids, the type 2 IGF receptor (IGF-2R), and IGF binding proteins 1-6 (18, 19).  The first three receptors initiate downstream signaling events, the IGF-2R sequesters local IGF-II, and the six IGFBPs regulate IGF-II activity in various tissues.

  1. LeRoith, D. & C.T. Roberts Jr. (2003) Cancer Lett. 195:127.
  2. Werner, H. & D. LeRoith (2000) Cell. Mol. Life Sci. 57:932.
  3. Pavelic, J. et al. (2007) Indian J. Med. Res. 125:511.
  4. Varela-Nieto, I. et al. (2007) Curr. Pharm. Des. 13:687.
  5. Rotwein, P. & L.J. Hall (1990) DNA Cell Biol. 10:725.
  6. Chao, W. & P. A. D-Amore (2008) Cytokine Growth Factor Rev. 19:111.
  7. Rotwein, P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:265.
  8. Goya, L. et al. (1999) J. Biol. Chem. 274:24633.
  9. McCarthy, T.L. et al. (1992) Endocrinology 130:1303.
  10. Zindy, F. et al. (1992) J. Hepatol. 14:30.
  11. Holthuizen, P.E. et al. (1993) Regul. Pept. 48:77.
  12. Merrick, D. et al. (2007) BMC Dev. Biol. 7:65.
  13. Stempien, M.M. et al. (1986) DNA 5:357.
  14. Ostrovsky, O. et al. (2009) Mol. Biol. Cell 20:1855.
  15. Daughaday, W.H. et al. (1993) Proc. Natl. Acad. Sci. USA 90:5823.
  16. Buchanan, C.M. et al. (2001) Biochem. J. 360:431.
  17. Cornish, J. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292:E117.
  18. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
  19. Belfiore, A. (2007) Curr. Pharm. Des. 13:671.
Long Name
Insulin-like Growth Factor II
Entrez Gene IDs
3481 (Human); 16002 (Mouse)
Alternate Names
C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; GRDF; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PEG2; PP9974; somatomedin-A

Citations for Recombinant Mouse IGF-II/IGF2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. HDAC I/IIb selective inhibitor Purinostat Mesylate combined with GLS1 inhibition effectively eliminates CML stem cells
    Authors: Q Qiu, L Yang, Y Feng, Z Zhu, N Li, L Zheng, Y Sun, C Pan, H Qiu, X Cui, W He, F Wang, Y Yi, M Tang, Z Yang, Y Yang, Z Li, L Chen, Y Hu
    Bioactive materials, 2023;21(0):483-498.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Insulin-like growth factor-2 does not improve behavioral deficits in mouse and rat models of Angelman Syndrome
    Authors: EL Berg, SP Petkova, HA Born, A Adhikari, AE Anderson, JL Silverman
    Molecular autism, 2021;12(1):59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Modulation of IGF2 Expression in the Murine Thymus and Thymic Epithelial Cells Following Coxsackievirus-B4 Infection
    Authors: H Michaux, A Halouani, C Trussart, C Renard, H Jaïdane, H Martens, V Geenen, D Hober
    Microorganisms, 2021;9(2):.
    Species: Mouse
    Sample Types: Reference Standard
    Applications: Western Blot
  4. Cell-Type-Specific Gene Regulatory Networks Underlying Murine Neonatal Heart Regeneration at Single-Cell Resolution
    Authors: Z Wang, M Cui, AM Shah, W Tan, N Liu, R Bassel-Dub, EN Olson
    Cell Reports, 2020;33(10):108472.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Glucoregulatory activity of vesiculin in insulin sensitive and resistant mice
    Authors: KL Lee, JF Aitken, HL Hsu, GM Williams, MA Brimble, GJS Cooper
    Peptides, 2019;116(0):1-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Immune targeting of autocrine IGF2 hampers rhabdomyosarcoma growth and metastasis
    Authors: C De Giovann, P Nanni, L Landuzzi, ML Ianzano, G Nicoletti, S Croci, A Palladini, PL Lollini
    BMC Cancer, 2019;19(1):126.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)
  7. Insulin-like growth factor 2 is a key mitogen driving liver repopulation in mice
    Authors: MJ Wang, F Chen, QG Liu, CC Liu, H Yao, B Yu, HB Zhang, HX Yan, Y Ye, T Chen, KJ Wangenstee, X Wang, YP Hu, ZY He
    Cell Death Dis, 2018;9(2):26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Identification of genes which regulate stroma-dependent in vitro hematopoiesis
    Authors: P Periasamy, V Tran, HC O'Neill
    PLoS ONE, 2018;13(10):e0205583.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Insulin-Like Growth Factor II Targets the mTOR Pathway to Reverse Autism-Like Phenotypes in Mice
    Authors: AB Steinmetz, SA Stern, AS Kohtz, G Descalzi, CM Alberini
    J. Neurosci., 2018;38(4):1015-1029.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Intranasal siRNA administration reveals IGF2 deficiency contributes to impaired cognition in Fragile X syndrome mice
    Authors: M Pardo, Y Cheng, D Velmeshev, M Magistri, H Eldar-Fink, A Martinez, MA Faghihi, RS Jope, E Beurel
    JCI Insight, 2017;2(6):e91782.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Pro-Insulin-Like Growth Factor-II Ameliorates Age-Related Inefficient Regenerative Response by Orchestrating Self-Reinforcement Mechanism of Muscle Regeneration.
    Authors: Ikemoto-Uezumi M, Uezumi A, Tsuchida K, Fukada S, Yamamoto H, Yamamoto N, Shiomi K, Hashimoto N
    Stem Cells, 2015;33(8):2456-68.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  12. TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions.
    Authors: Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S
    PLoS ONE, 2015;10(1):e0117908.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Hedgehog signaling activates a positive feedback mechanism involving insulin-like growth factors to induce osteoblast differentiation.
    Authors: Shi Y, Chen J, Karner C, Long F
    Proc Natl Acad Sci U S A, 2015;112(15):4678-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Identification and Characterization of Lineage(-)CD45(-)Sca-1(+) VSEL Phenotypic Cells Residing in Adult Mouse Bone Tissue.
    Authors: Nakatsuka R, Iwaki R, Matsuoka Y, Sumide K, Kawamura H, Fujioka T, Sasaki Y, Uemura Y, Asano H, Kwon A, Sonoda Y
    Stem Cells Dev, 2015;25(1):27-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Insulin-like Growth Factor 2 Overexpression Induces beta-Cell Dysfunction and Increases Beta-cell Susceptibility to Damage.
    Authors: Casellas A, Mallol C, Salavert A, Jimenez V, Garcia M, Agudo J, Obach M, Haurigot V, Vila L, Molas M, Lage R, Morro M, Casana E, Ruberte J, Bosch F
    J Biol Chem, 2015;290(27):16772-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Insulin-like growth factor 2 enhances regulatory T-cell functions and suppresses food allergy in an experimental model.
    Authors: Yang G, Geng X, Song J, Wu Y, Yan H, Zhan Z, Yang L, He W, Liu Z, Qiu S, Liu Z, Yang P
    J Allergy Clin Immunol, 2014;133(6):1702-8.e5.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. Insulin-like growth factor-II (IGF-II) and IGF-II analogs with enhanced insulin receptor-a binding affinity promote neural stem cell expansion.
    Authors: Ziegler A, Chidambaram S, Forbes B, Wood T, Levison S
    J Biol Chem, 2014;289(8):4626-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. A critical role for IGF-II in memory consolidation and enhancement.
    Authors: Chen DY, Stern SA, Garcia-Osta A, Saunier-Rebori B, Pollonini G, Bambah-Mukku D, Blitzer RD, Alberini CM
    Nature, 2011;469(7331):491-7.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  19. The chaperone activity of GRP94 toward insulin-like growth factor II is necessary for the stress response to serum deprivation.
    Authors: Ostrovsky O, Ahmed NT, Argon Y
    Mol. Biol. Cell, 2009;20(6):1855-64.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  20. Insulin-like growth factor-binding protein 2 secreted by a tumorigenic cell line supports ex vivo expansion of mouse hematopoietic stem cells.
    Authors: Huynh H, Iizuka S, Kaba M, Kirak O, Zheng J, Lodish HF, Zhang CC
    Stem Cells, 2008;26(6):1628-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice.
    Authors: Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA
    Stem Cells, 2008;26(7):1778-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Hypoxia-independent overexpression of hypoxia-inducible factor 1alpha as an early change in mouse hepatocarcinogenesis.
    Authors: Tanaka H, Yamamoto M, Hashimoto N, Miyakoshi M, Tamakawa S, Yoshie M, Tokusashi Y, Yokoyama K, Yaginuma Y, Ogawa K
    Cancer Res., 2006;66(23):11263-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay


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