Recombinant Mouse IGFBP-4 Protein

Carrier Free

Catalog # Availability Size / Price Qty
8066-GB-025/CF

With Carrier

Catalog # Availability Size / Price Qty
8066-GB-025
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse IGFBP-4 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.02-0.12 μg/mL in the presence of 14 ng/mL of Recombinant Mouse IGF‑II (Catalog # 792-MG).
Source
Mouse myeloma cell line, NS0-derived mouse IGFBP-4 protein
Asp22-Glu254
Accession #
N-terminal Sequence
Analysis
Asp22
Predicted Molecular Mass
26 kDa
SDS-PAGE
30-36 kDa, reducing conditions

Product Datasheets

8066-GB (with carrier)

8066-GB/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8066-GB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8066-GB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGFBP-4

IGFBP‑4 (insulin‑like growth factor binding protein 4) is member of the IGFBP family of structurally similar secreted glycoproteins that maintain IGF‑I and IGF‑II in the circulation and direct them to their target tissues (1, 2). Mouse IGFBP‑4 cDNA encodes 254 amino acids (aa) that include a 21 aa signal peptide and a 233 aa cysteine-rich mature protein that contains an N‑terminal IGFBP domain, a linker sequence, and a C‑terminal thyroglobulin domain. Mature mouse IGFBP‑4 shares 99% aa sequence identity with rat IGFBP‑4, and 90‑91% with human, canine, bovine, ovine and porcine IGFBP‑4. Both domains interact with IGFs, although the N‑terminal domain is primary (2). The linker contains a cleavage site that is accessible by PAPP‑A (pregnancy-associated plasma protein A) when IGFs are bound (2, 3). IGFBP‑4 fragments detected in the plasma of pregnant women have lowered affinity for IGFs, thus up‑regulating IGF bioavailability for receptor binding (2). Unlike other family members, IGFBP‑4 does not interact with the extracellular matrix or translocate to the nucleus (2). Like other family members, it is expressed early in development and has both IGF‑dependent and ‑independent activities (2, 4). IGFBP‑4 expression in mesenchymal stem cells is up‑regulated by the cardiac transcription factor GATA‑4 (5). It then enhances cardiomyocyte differentiation by interacting with Frizzled and LRP molecules, inhibiting canonical Wnt signaling (6, 7). IGF can sequester IGFBP‑4 when present, inhibiting its ability to support cardiomyocyte differentiation (3, 4). IGFBP‑4 is also up‑regulated in osteoblasts in response to estrogen (8). Intact or proteolytically cleaved IGFBP‑4 may either promote or inhibit cancer cell growth, motility and invasion depending on cell type, tissue and stage of the cancer (9‑11). It is shown to inhibit IGF‑1‑ or FGF‑2‑induced angiogenesis, but not that induced by VEGF (10).

References
  1. Bethel, C.R. et al. (1994) Biochem. Mol. Biol. Int. 34:385.
  2. Forbes, B.E. et al. (2012) Front. Endocrinol. (Lausanne) 3:38.
  3. Gyrup, C. and C. Oxvig  (2007) Biochemistry 46:1972.
  4. Schuller, A.G. et al. (1994) Mol. Cell. Endocrinol. 104:57.
  5. Li, H. et al. (2011) Cytotherapy 13:1057.
  6. Zhu, W. et al. (2008) Nature 454:345.
  7. Minato, A. et al. (2012) Biomaterials 33:515.
  8. Denger, S. et al. (2008) Mol. Endocrinol. 22:361.
  9. Ueno, K. et al. (2011) Int. J. Cancer 129:2360.
  10. Contois, L.W. et al. (2012) J. Biol. Chem. 287:1779.
  11. Moreno, M.J. et al. (2013) Neoplasia 15:554.
Long Name
Insulin-like Growth Factor Binding Protein 4
Entrez Gene IDs
3487 (Human); 16010 (Mouse)
Alternate Names
BP-4; HT29-IGFBP; IBP4insulin-like growth factor-binding protein 4; IGF-binding protein 4; IGFBP4; IGFBP-4; IGFBP-4IBP-4; insulin-like growth factor binding protein 4

Citation for Recombinant Mouse IGFBP-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Insulin-like growth factor 1 stimulates the angiogenic activity of adipose tissue-derived microvascular fragments
    Authors: MW Laschke, E Kontaxi, C Scheuer, A He beta, P Karschnia, MD Menger
    J Tissue Eng, 2019;10(0):2041731419879.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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