Recombinant Mouse IL-12 R beta 2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
7406-MR-050

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Recombinant Mouse IL-12 R beta 2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse (rm) IL‑12 R beta 2 Fc Chimera is immobilized at 0.5 μg/mL (100 μL/well), the concentration of rmIL-12 that produces 50% of the optimal binding response is found to be approximately 4 ‑ 20 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-12 R beta 2 protein
Mouse IL-12 R beta 2
(Met1-Asn637)
Accession # P97378
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asn24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
95.7 kDa (monomer)
SDS-PAGE
125-145 kDa, reducing conditions

Product Datasheets

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7406-MR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7406-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IL-12 R beta 2

The high‑affinity IL‑12 receptor complex includes the 100 kDa IL‑12 receptor beta 1 (IL‑12 R beta 1) and the 130 kDa IL‑12 Receptor  beta 2 (IL‑12 R beta 2) subunits, both of which are type I transmembrane proteins that belong to the cytokine receptor superfamily (1, 2). The complex's ligand, IL‑12, is a disulfide‑linked heterodimer composed of 35 kDa (IL‑12 alpha  p35) and 40 kDa (IL‑12 beta  p40) subunits. IL‑12 R beta 2 binds IL‑12 alpha and signals through Jak2, while IL‑12 R beta 1 binds IL‑12 beta and signals through Tyk2 (3). IL‑12 R beta 1 is also a subunit of the IL‑23 receptor complex (3). The 874 amino acid (aa) mouse IL‑12 R beta 2 precursor includes a 23 aa signal peptide, a 614 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 216 aa cytoplasmic region. The ECD possesses one C2‑type Ig‑like domain, five fibronectin type III (Fn III) repeats, 14 potential N‑glycosylation sites, and a WSXWS motif, while the cytoplasmic region contains a Box 1 motif and three tyrosine phosphorylation sites that presumably mediate intracellular signaling (3). The mouse IL‑12 R beta 2 ECD shares 91% aa sequence identity with rat IL‑12 R beta 2, and 68% with human, porcine and bovine IL‑12 R beta 2. Human and mouse IL‑12 R beta 2 do not bind cross‑species IL‑12 (2). A 734 aa mouse isoform that lacks aa 363‑503 within the Fn III domains is reported (4). Unlike IL‑12 R beta 1, which is constitutively expressed on T cells, NK cells and B cells, IL‑12 R beta 2 expression is more restricted (2). On naïve T cells, IL‑12 R beta 2 is expressed following STAT1 activation by IFN‑ gamma, IL‑27 and/or T cell receptor ligation. This up‑regulation allows IL‑12 to promote Th1, but not Th2, differentiation (5‑7). Among B cells, surface expression is limited to naïve germinal center and memory B cells, and myeloma cells (2). Deletion of IL‑12 R beta 2 causes systemic over-expression of IL‑6, accelerated maturation of thymocytes, deficient regulatory T cell maturation and function, and reduced splenic T cell apoptosis (2, 8-10). These mice are susceptible to autoimmune diseases such as experimental autoimmune encephalitis and spontaneous B cell malignancies (2, 8-10). In humans, polymorphism of the IL‑12 R beta 2 gene is associated with systemic sclerosis (11).

References
  1. Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:14002.
  2. Pistoia, V. et al. (2009) J. Clin. Oncol. 27:4809.
  3. Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
  4. GenBank protein Accession # P97378.
  5. Rogge, L. et al. (1997) J. Exp. Med. 185:825.
  6. Becskei, A. and M.J. Grusby (2007) FEBS Lett. 581:5199.
  7. Szabo, S.J. et al. (1997) J. Exp. Med. 185:817.
  8. Zhao, Z. et al. (2008) J. Immunol. 181:3870.
  9. Gran, B. et al. (2010) Exp. Mol. Pathol. 89:126.
  10. Airoldi, I. et al. (2005) Blood 106:3846.
  11. Bossini-Castillo, L. et al. (2011) Hum. Mol. Genet. Nov. 29 [Epub ahead of print].
Long Name
Interleukin 12 Receptor beta 2
Entrez Gene IDs
3595 (Human); 16162 (Mouse)
Alternate Names
IL-12 R beta 2; IL-12 receptor beta 2; IL-12 receptor subunit beta-2; IL12R beta 2; IL-12R subunit beta-2; IL12RB2; IL-12Rb2; IL-12R-beta-2; interleukin 12 receptor, beta 2; interleukin-12 receptor beta-2 chain; interleukin-12 receptor subunit beta-2

Citation for Recombinant Mouse IL-12 R beta 2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Tryptophan (W) at position 37 of murine IL-12/IL-23 p40 is mandatory for binding to IL-12Rbeta1 and subsequent signal transduction
    Authors: J Georgy, Y Arlt, JM Moll, M Ouzin, HT Weitz, L Gremer, D Willbold, J Grötzinger, F Thives-Kur, J Scheller, DM Floss
    The Journal of Biological Chemistry, 2021;0(0):101295.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Surface Plasmon Resonance

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