Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF

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Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera Bioactivity
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Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑18 R alpha /IL‑1 R5 Chimera is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Mouse IL-18 (Catalog # 9139-CL) that produces 50% of the optimal binding response is 1.5-9 μg/mL.
Mouse myeloma cell line, NS0-derived mouse IL-18 R alpha/IL-1 R5 protein
Mouse IL-18 R alpha/IL-1 R5
Accession # Q61098
IEGRDP Mouse IgG2a
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
62 kDa
90-106 kDa, reducing conditions

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Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera Bioactivity View Larger

When Recombinant Mouse IL-18 Ra/IL-1 R5 Fc Chimera (Catalog # 9285-BP) is coated at 2 µg/mL, 100 µL/well, Recombinant Mouse IL-18/IL-1F4 (Catalog # 9139-IL) binds with a ED50 of 1.5-9 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: IL-18 R alpha/IL-1 R5

IL-18 Receptor alpha (IL-18 Ra), also known as IL-18 R1, IL-1 R5, and IL-1 Rrp, is a transmembrane component of the IL-18 Receptor (1). It associates with IL‑18 Rb/IL‑1 R7 to form the high affinity receptor complex for IL-18 and confer cellular responsiveness to IL-18 (2, 3). IL-18 is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. Mature mouse IL-18 Ra consists of a 307 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain (4). Within the ECD, mouse IL-18 Ra shares 56% and 82% aa sequence identity with human and rat IL-18 Ra, respectively. IL-18 Ra is widely expressed on epithelial cells as well as on B cells, NK cells, CD4+ and CD8+ T cells, and dendritic cells (5-8). Signaling through the IL-18 receptor promotes a Th1 biased immune response and NK cell cytolytic activity (6, 7, 9-11). It also contributes to cigarette smoke-induced airway inflammation and epithelial damage (12).

  1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
  2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  4. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
  5. Kunikata, T. et al. (1998) Cell Immunol. 189:135.
  6. Smeltz, R.B. et al. (2001) J. Exp. Med. 194:143.
  7. Chan, W.L. et al. (2001) J. Immunol. 167:1238.
  8. Kaser, A. et al. (2004) Blood 103:648.
  9. Tomura, M. et al. (1998) J. Immunol. 160:3759.
  10. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  11. Hyodo, Y. et al. (1999) J. Immunol. 162:1662.
  12. Kang, M.J. et al. (2007) J. Immunol. 178:1948.
Long Name
Interleukin 18 Receptor alpha
Entrez Gene IDs
8809 (Human); 16182 (Mouse)
Alternate Names
CD218a; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra


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