Recombinant Mouse IL-18 R alpha/IL-1 R5 Fc Chimera, CF Summary
|Mouse IL-18 R alpha/IL-1 R5 |
Accession # Q61098
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 1 mg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Mouse IL-18 Ra/IL-1 R5 Fc Chimera (Catalog # 9285-BP) is coated at 2 µg/mL, 100 µL/well, Recombinant Mouse IL-18/IL-1F4 (Catalog # 9139-IL) binds with a ED50 of 1.5-9 µg/mL.
Background: IL-18 R alpha/IL-1 R5
IL-18 Receptor alpha (IL-18 Ra), also known as IL-18 R1, IL-1 R5, and IL-1 Rrp, is a transmembrane component of the IL-18 Receptor (1). It associates with IL‑18 Rb/IL‑1 R7 to form the high affinity receptor complex for IL-18 and confer cellular responsiveness to IL-18 (2, 3). IL-18 is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. Mature mouse IL-18 Ra consists of a 307 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain (4). Within the ECD, mouse IL-18 Ra shares 56% and 82% aa sequence identity with human and rat IL-18 Ra, respectively. IL-18 Ra is widely expressed on epithelial cells as well as on B cells, NK cells, CD4+ and CD8+ T cells, and dendritic cells (5-8). Signaling through the IL-18 receptor promotes a Th1 biased immune response and NK cell cytolytic activity (6, 7, 9-11). It also contributes to cigarette smoke-induced airway inflammation and epithelial damage (12).
- Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
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- Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
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- Kunikata, T. et al. (1998) Cell Immunol. 189:135.
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- Chan, W.L. et al. (2001) J. Immunol. 167:1238.
- Kaser, A. et al. (2004) Blood 103:648.
- Tomura, M. et al. (1998) J. Immunol. 160:3759.
- Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
- Hyodo, Y. et al. (1999) J. Immunol. 162:1662.
- Kang, M.J. et al. (2007) J. Immunol. 178:1948.
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