Recombinant Mouse IL-31 RA Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1253-IL-050
Recombinant Mouse IL-31 RA Fc Chimera Protein, CF SDS-PAGE
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Recombinant Mouse IL-31 RA Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑31 RA Fc Chimera is immobilized at 5 µg/mL (100 µL/well), Recombinant Mouse IL‑31 (Catalog # 3028-ML) binds with an ED50 of 0.9-5.4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-31 RA protein
Mouse IL-31RA
(Val19-Ser503)
Accession # AAM27959.1
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Val19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
82 kDa
SDS-PAGE
90-103 kDa, reducing conditions

Product Datasheets

1253-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE Recombinant Mouse IL-31 RA Fc Chimera Protein, CF SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse IL‑31 RA was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by silver staining,showing bands at 90-103 kDa and 180-210 kDa, respectively.

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Background: IL-31 RA

The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100 kDa type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are up-regulated by interferon-gamma and bacterial lipopolysaccharides (1-3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3-6). The 716 amino acid (aa) mouse IL-31 RA contains an 18 aa signal sequence, a 481 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 196 aa cytoplasmic domain. The ECD of mouse IL-31 RA shares 60% and 84% aa identity with human and rat IL-31 RA ECD, respectively. Mouse IL-31 receptors do not respond to human IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2).  In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 8, 9).

References
  1. Ghilardi, N. et al. (2002) J. Biol. Chem. 277:16831.
  2. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
  3. Dillon, S. R. et al. (2004) Nat. Immunol. 5:752.
  4. Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
  5. Perrigoue, J. G. et al. (2007) J. Exp. Med. 204:481.
  6. Bando, T. et al. (2006) Neuroscience 142:1263.
  7. Broxmeyer, H. E. et al. (2007) Exp. Hematol. 35:78.
  8. Bilsborough, J. et al. (2006) J. Allergy Clin. Immunol. 117:418.
  9. Sonkoly, E. et al. (2006) J. Allergy Clin. Immunol. 117:411.
Long Name
Interleukin 31 Receptor A
Entrez Gene IDs
133396 (Human); 218624 (Mouse)
Alternate Names
class I cytokine receptor; CRL3; CRL3Glmr; Cytokine receptor-like 3; GLMR; GLM-R; GLM-RMGC125346; GP130 like receptor; Gp130-like monocyte receptor; Gp130-like receptor; hGLM-R; IL-31 RA; IL-31 receptor subunit alpha; IL-31R subunit alpha; IL31RA; IL-31RA; IL-31RAGPLCRL; IL-31R-alpha; interleukin 31 receptor A; interleukin-31 receptor subunit alpha; PRO21384; soluble type I cytokine receptor CRL3; zcytoR17

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