Recombinant Mouse IL-31 Protein, CF

Catalog # Availability Size / Price Qty
3028-ML-010
Recombinant Mouse IL-31 Protein Binding Activity
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Citations (1)
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Recombinant Mouse IL-31 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑31 RA Fc Chimera (Catalog # 1253-IL) is immobilized at 5 µg/mL (100 µL/well), Recombinant Mouse IL‑31 binds with an ED50 of 0.9-5.4 μg/mL.
Source
E. coli-derived mouse IL-31 protein
Thr24-Cys163
Accession #
N-terminal Sequence
Analysis
Thr24
Predicted Molecular Mass
15.6 kDa
SDS-PAGE
13 kDa, reducing conditions

Product Datasheets

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3028-ML

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3028-ML

Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid and NaCl.
Reconstitution Reconstitute at 100 μg/mL in 10 mM Acetic Acid.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse IL-31 Protein Binding Activity View Larger

When Recombinant Mouse IL-31 RA Fc Chimera (1253-IL) is immobilized at 5 µg/mL, Recombinant Mouse IL-31 (Catalog # 3028-ML) binds with an ED50 of 0.9-5.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: IL-31

Mouse Interleukin-31 (IL-31) is likely a secreted, 24‑33 kDa short-chain member of the alpha -helical IL-6 family of cytokines (1, 2). The mouse IL‑31 cDNA encodes a 163 amino acid (aa) precursor that contains a 23 aa signal peptide and a 140 aa mature protein (3, 4). The mature region shows four alpha ‑helices which would be expected to generate a typical up-up-down-down topology. There are three potential sites for N‑linked glycosylation. Mature mouse IL-31 shares 29% and 63% aa sequence identity with human and rat IL-31, respectively. Neither mouse nor human IL-31 are active on their counterparts receptors (1). IL-31 is associated with activated T cells and is preferentially expressed by Th2 rather than Th1 cells (1). IL-31 signals via a heterodimeric receptor complex composed of a newly identified, 120 kDa, gp130-related molecule termed IL-31 RA (also GPL and GLM-R) and the 180 kDa oncostatin M receptor (OSM R beta ) (4‑8). In the complex, IL‑31 directly binds to IL‑31 RA, not OSM R (4, 5). IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and MAP kinase pathway. Although multiple isoforms of IL-31 RA are known, only a form that contains the entire length of the cytoplasmic domain is signaling-capable (4‑7). The IL-31 receptor is constitutively expressed by keratinocytes and upregulated by IFN-gamma on monocytes (1, 3). Other cells known to be responsive to IL-31 include bronchial epithelium, type II alveolar cells, goblet cells, and likely hematopoietic progenitor cells (9‑11). Functionally, it has been shown that IL-31 may contribute to clinical pruritis (itching) associated with nonatopic dermatitis (1, 3, 12). This may be a consequence of IL-31’s ability to induce keratinocyte secretion of multiple cytokines, including TARC, GRO‑ alpha, MIP‑3 beta and I-309 (1). In addition, IL-31 promotes proliferation of high density cell populations such as myeloid progenitor cells, but conversely suppresses proliferation of lung epithelial cells (1). Finally, IL-31 may actually have a modulating effect on T cell subsets, influencing the ratio between Th1 and Th2 cells (1).

References
  1. Zhang, Q. et al. (2008) Cytokine Growth Factor Rev. 19:347.
  2. Venereau, E. et al. (2009) J. Biol. Chem. 285:14955.
  3. Dillon SR, et al. (2004) Nat. Immunol. 5:752.
  4. Diveu C, et al. (2004) Eur. Cytokine Netw. 15:291.
  5. Dreuw A, et al. (2004) J. Biol. Chem. 279:36112.
  6. Dieu C, et al. (2003) J. Biol. Chem. 278:49850.
  7. Ghilardi N, et al. (2002) J. Biol. Chem 277:16831.
  8. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
  9. Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
  10. Perrigoue, J.G. (2009) J. Immunol. 182:6088.
  11. Broxmeyer, H.E. et al. (2007) Exp. Hematol. 35 (Suppl 1):78.
  12. Takaoka, A. et al. (2005) Eur. J. Pharmacol. 516:180.
Long Name
Interleukin 31
Entrez Gene IDs
386653 (Human); 76399 (Mouse)
Alternate Names
IL31; IL-31; IL-31interleukin-31; interleukin 31

Citation for Recombinant Mouse IL-31 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010-09-29;84(24):12703-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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Recombinant Mouse IL-31 Protein, CF
By Katie Myers on 11/14/2022
Application: Immunoassay Standard
Reason for Rating: There is very little cross-reactivity in our standard when we use this analyte.

Worked well as calibration material for our ELISA.