Recombinant Mouse LAG-3 Fc Chimera Protein, CF

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3328-L3-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse LAG-3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce TNF-alpha secretion by JAWSII mouse immature dendritic cells. The ED50 for this effect is 0.4-2.4 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse LAG-3 protein
Mouse LAG-3
(Gly24-Leu442)
Accession # Q61790
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Gly24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
72.4 kDa (monomer)
SDS-PAGE
85-100 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3328-L3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.  Reconstitute 30 minutes prior to use with minimal agitation. 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
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Background: LAG-3

LAG-3 (Lymphocyte activation gene-3), designated CD223, is a 70 kDa type I transmembrane protein that is a member of the immunoglobulin superfamily (IgSF) (1, 2). LAG-3 shares approximately 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity, providing negative regulation of T cell receptor signaling (1, 2). Mouse LAG-3 cDNA encodes 521 amino acids (aa) that include a 22 aa signal sequence, a 420 aa extracellular domain (ECD) with four Ig-like domains, a  transmembrane region and a highly charged cytoplasmic region. Within the ECD, mouse LAG-3 shares 86% aa sequence identity with rat LAG-3, and 65-69% with human, porcine, and bovine LAG-3. LAG-3 is expressed on activated CD4+ and CD8+ T cells, NK cells, and plasmacytoid dendritic cells (pDC), but not on resting T cells (1-3). LAG-3 on activated CD4+CD25+ Treg cells plays a role in their suppressive activity (4). LAG-3 limits the expansion of activated T cells and pDC in response to selected stimuli (3-5). A soluble 54 kDa form, sLAG-3, can be shed by metalloproteinases ADAM10 and TACE/ADAM17 (6, 7). While monomeric sLAG-3 itself may be inactive, shedding allows for normal T cell activation by removing negative regulation (7). Binding of a homodimerized sLAG-3/Ig fusion protein to MHC class II molecules induces maturation of immature DC, and secretion of cytokines such as IFN-gamma and TNF-alpha by type 1 cytotoxic CD8+ T cells and NK cells (8, 9). sLAG-3/Ig has been used as a potential adjuvant to stimulate a cytotoxic anti-cancer immune response (9, 10). In mice, deletion of LAG-3 and another negative regulator, PD-1, facilitates anti-cancer response but also blocks self-tolerance and increases susceptibility to autoimmune diseases (11, 12). In humans, antibody-mediated down‑regulation of LAG-3 and PD-1 allows more effective control of chronic malaria, while in NOD (non‑obese diabetic) mice, deletion of LAG-3 alone accelerates diabetes (12-14).

References
  1. Triebel, F. et al. (1990) J. Exp. Med. 171:1393.
  2. Baixeras, E. et al. (1992) J. Exp. Med 176:327.
  3. Workman, C.J. et al. (2004) J. Immunol. 172:5450.
  4. Huang, C.T. et al. (2004) Immunity 21:503.
  5. Workman, C.J. et al. (2009) J. Immunol. 182:1885.
  6. Li, N. et al. (2004) J. Immunol. 173:6806.
  7. Li, N. et al. (2007) EMBO J. 26:494.
  8. Andreae, S. et al. (2003) Blood 102:2130.
  9. Brignone, C. et al. (2007) J. Immunol. 179:4202.
  10. Brignone, C. et al. (2010) J. Transl. Med. 8:71.
  11. Woo, S.R. et al. (2011) Cancer Res. 72:917.
  12. Okazaki, T. et al. (2011) J. Exp. Med. 208:395.
  13. Bettini, M. et al. (2011) J. Immunol. 187:3493.
  14. Butler, N.S. et al. (2012) Nat. Immunol. 13:188.
Long Name
Lymphocyte-activation Gene 3
Entrez Gene IDs
3902 (Human); 16768 (Mouse); 297596 (Rat); 102122272 (Cynomolgus Monkey)
Alternate Names
CD223 antigen; CD223; LAG3; LAG-3; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG-3

Citation for Recombinant Mouse LAG-3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification of LAG3 high affinity aptamers by HT-SELEX and Conserved Motif Accumulation (CMA)
    Authors: MM Soldevilla, S Hervas, H Villanueva, T Lozano, O Rabal, J Oyarzabal, JJ Lasarte, M Bendandi, S Inoges, A López-Díaz, F Pastor
    PLoS ONE, 2017;12(9):e0185169.
    Species: NA
    Sample Types: Aptamer
    Applications: Aptamer Screening

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