Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF

Catalog # Availability Size / Price Qty
9095-T4-050
Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF Bioactivity
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Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse LILRB4/CD85k/ILT3 is coated onto a microplate at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 = 20-120 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse LILRB4/CD85k/ILT3 protein
Gly24-Lys238, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gly24 & Ser37 (minor)
Predicted Molecular Mass
25 kDa
SDS-PAGE
34-42 kDa, reducing conditions

Product Datasheets

9095-T4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF Bioactivity View Larger

When Recombinant Mouse LILRB4/CD85k/
ILT3 is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7 (Catalog # 914-AN) binds with a typical ED50 of
20-120 ng/mL.

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Background: LILRB4/CD85k/ILT3

LILRB4, also known as ILT3, CD85k, and LIR-5, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation (1). Mature mouse LILRB4 consists of a 215 amino acid (aa) extracellular domain with two Ig-like domains, a 22 aa transmembrane segment, and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). Within the ECD, mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4, respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment (2). LILRB4 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC) (3, 6, 7). Ligation of LILRB4 triggers ITIM-mediated inhibition of cell-activating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection (3, 5, 8, 9). Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL (5, 8, 10). In turn, CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC (6, 7, 9, 11, 12).

References
  1. Vlad, G. et al. (2010) Int. Rev. Immunol. 29:119.
  2. Castells, M.C. et al. (1994) J. Biol. Chem. 269:8393.
  3. Cella, M. et al. (1997) J. Exp. Med. 185:1743.
  4. Heinzmann, A. et al. (2000) Eur. J. Immunogenet. 27:121.
  5. Suciu-Foca, N. et al. (2007) J. Immunol. 178:7432.
  6. Gleissner, C.A. et al. (2007) Eur. J. Immunol. 37:177.
  7. Manavalan, J.S. et al. (2004) Int. Immunol. 16:1055.
  8. Vlad, G. and N. Suciu-Foca (2012) Exp. Mol. Pathol. 93:294.
  9. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
  10. Vlad, G. et al. (2006) Int. Immunopharmacol. 6:1889.
  11. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.
  12. Brenk, M. et al. (2009) J. Immunol. 183:145.
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4
Entrez Gene IDs
11006 (Human); 14728 (Mouse)
Alternate Names
CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB4; LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4

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