Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse LILRB4/CD85k/ILT3 is coated onto a microplate at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 = 20-120 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived mouse LILRB4/CD85k/ILT3 protein
    Gly24-Lys238, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Gly24 & Ser37 (minor)
  • Predicted Molecular Mass
    25 kDa
  • SDS-PAGE
    34-42 kDa, reducing conditions
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9095-T4
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 500 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
When Recombinant Mouse LILRB4/CD85k/
ILT3 is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7 (Catalog # 914-AN) binds with a typical ED50 of
20-120 ng/mL.
Background: LILRB4/CD85k/ILT3
LILRB4, also known as ILT3, CD85k, and LIR-5, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation (1). Mature mouse LILRB4 consists of a 215 amino acid (aa) extracellular domain with two Ig-like domains, a 22 aa transmembrane segment, and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). Within the ECD, mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4, respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment (2). LILRB4 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC) (3, 6, 7). Ligation of LILRB4 triggers ITIM-mediated inhibition of cell-activating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection (3, 5, 8, 9). Soluble LILRB4 induces the differentiation of CD8+ T suppressor cells (Ts) that can inhibit the effector functions of CD4+ Th cells and CD8+ CTL (5, 8, 10). In turn, CD8+ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC (6, 7, 9, 11, 12).
  • References:
    1. Vlad, G. et al. (2010) Int. Rev. Immunol. 29:119.
    2. Castells, M.C. et al. (1994) J. Biol. Chem. 269:8393.
    3. Cella, M. et al. (1997) J. Exp. Med. 185:1743.
    4. Heinzmann, A. et al. (2000) Eur. J. Immunogenet. 27:121.
    5. Suciu-Foca, N. et al. (2007) J. Immunol. 178:7432.
    6. Gleissner, C.A. et al. (2007) Eur. J. Immunol. 37:177.
    7. Manavalan, J.S. et al. (2004) Int. Immunol. 16:1055.
    8. Vlad, G. and N. Suciu-Foca (2012) Exp. Mol. Pathol. 93:294.
    9. Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
    10. Vlad, G. et al. (2006) Int. Immunopharmacol. 6:1889.
    11. Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.
    12. Brenk, M. et al. (2009) J. Immunol. 183:145.
  • Long Name:
    Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4
  • Entrez Gene IDs:
    11006 (Human); 14728 (Mouse)
  • Alternate Names:
    CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB4; LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4
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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 28  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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