Recombinant Mouse MCAM/CD146 Fc Chimera Protein, CF Summary
Accession # Q8R2Y2-1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Melanoma cell adhesion molecule (MCAM), also known as MUC18 or CD146, is a member of the immunoglobulin superfamily (IgSF), showing close sequence similarity to neural cell adhesion molecules (1). MCAM is a type I transmembrane glycoprotein that consists of a 23 aa signal peptide, a 540 aa extracellular domain (ECD), a 21 aa transmembrane segment and 64 aa cytoplasmic domain. Within the ECD, mouse MCAM shares 74% and 90% amino acid sequence identity with human and rat MCAM, respectively. Expression of MCAM has been detected in endothelial cells throughout the body and it has been shown to be involved in multiple cellular events including adhesion, migration, proliferation and differentiation (2, 3). Additionally, MCAM has been implicated in recruitment of activated T cells to inflammatory sites and is up-regulated in various inflammatory diseases (3, 4). Inhibiting MCAM signaling has been suggested as a potential therapy for diverse diseases including inflammatory bowel disease and ovarian cancer (5, 6). As a cellular adhesion molecule (CAM), MCAM functions as a molecular mediator to facilitate inter-cellular interactions of homotypic or heterotypic cells, or to intervene in interactions of cell-to-extracellular matrix for responding to physiological signal (7). MCAM has also been shown to be the functional ligand for Galectin-3 on endothelial cell surfaces (7).
- Lehmann, J.M. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 86:9891.
- Schrage, A. et al. (2008) Histochem Cell Biol. 129:441.
- Wang, Z. and Yan X. (2013) Cancer Lett. 330:150.
- Bardin, N. et al. (2006) Inflamm Bowel Dis. 12:16.
- Xing, S. et al. (2014) Am J Pathol. 184:1604.
- Ma, X. et al. (2017) Oncol Lett. 13:1681.
- Colomb, F. et al. (2017) J Biol Chem. 292:8381.
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