Recombinant Mouse MCAM/CD146 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9809-MA-050
Recombinant Mouse MCAM/CD146 Fc Chimera Protein Binding Activity
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Recombinant Mouse MCAM/CD146 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Mouse Galectin-3 (Catalog # 9039-GA) that produces 50% of the optimal binding response is 0.075-0.375 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse MCAM/CD146 protein
Mouse MCAM
(Val24-Val563)
Accession # Q8R2Y2-1
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Val24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
87 kDa
SDS-PAGE
112-129 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9809-MA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Mouse MCAM/CD146 Fc Chimera Protein Binding Activity View Larger

When Recombinant Mouse MCAM/CD146 Fc Chimera (Catalog # 9809-MA) is coated at 1 µg/mL (100 µg/mL), Recombinant Mouse Galectin-3 (Catalog # 9039-GA) binds with an ED50 of 0.075-0.375 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MCAM/CD146

Melanoma cell adhesion molecule (MCAM), also known as MUC18 or CD146, is a member of the immunoglobulin superfamily (IgSF), showing close sequence similarity to neural cell adhesion molecules (1). MCAM is a type I transmembrane glycoprotein that consists of a 23 aa signal peptide, a 540 aa extracellular domain (ECD), a 21 aa transmembrane segment and 64 aa cytoplasmic domain. Within the ECD, mouse MCAM shares 74% and 90% amino acid sequence identity with human and rat MCAM, respectively. Expression of MCAM has been detected in endothelial cells throughout the body and it has been shown to be involved in multiple cellular events including adhesion, migration, proliferation and differentiation (2, 3). Additionally, MCAM has been implicated in recruitment of activated T cells to inflammatory sites and is up-regulated in various inflammatory diseases (3, 4). Inhibiting MCAM signaling has been suggested as a potential therapy for diverse diseases including inflammatory bowel disease and ovarian cancer (5, 6). As a cellular adhesion molecule (CAM), MCAM functions as a molecular mediator to facilitate inter-cellular interactions of homotypic or heterotypic cells, or to intervene in interactions of cell-to-extracellular matrix for responding to physiological signal (7). MCAM has also been shown to be the functional ligand for Galectin-3 on endothelial cell surfaces (7).

References
  1. Lehmann, J.M. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 86:9891.
  2. Schrage, A. et al. (2008) Histochem Cell Biol. 129:441.
  3. Wang, Z. and Yan X. (2013) Cancer Lett. 330:150.
  4. Bardin, N. et al. (2006) Inflamm Bowel Dis. 12:16.
  5. Xing, S. et al. (2014) Am J Pathol. 184:1604.
  6. Ma, X. et al. (2017) Oncol Lett. 13:1681.
  7. Colomb, F. et al. (2017) J Biol Chem. 292:8381.
Long Name
Melanoma Cell Adhesion Molecule
Entrez Gene IDs
4162 (Human); 84004 (Mouse); 78967 (Rat)
Alternate Names
CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin

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