Recombinant Mouse MESDC2 Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Supplemental Products

Recombinant Mouse MESDC2 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The typical ND50 for this effect is 2-8 µg/mL.
E. coli-derived mouse MESDC2 protein
Accession #
N-terminal Sequence
Predicted Molecular Mass
22 kDa

Complete Your Experiment

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: MESDC2

Mesoderm development candidate gene 2 (MESDC2, Mesd), also known as Boca in drosophila, is a 22 kDa protein that is required for formation of the primitive streak and mesoderm during embryogenesis (1 - 3). Mature mouse MESDC2 consists of an 83 amino acid (aa) structured central domain with flexible N- and C-terminal regions (4, 5). It shares 89% and 96% aa sequence identity with human and rat MESDC2, respectively. Although Boca lacks 34 aa in the C-terminal region and is only 40% identical with mouse MESDC2, the mouse protein can functionally substitute for Boca in drosophila S2 cells (6). A C-terminal ER retention motif localizes MESDC2 and Boca to the lumen of the endoplasmic reticulum (2, 6, 7). Within the ER, MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 and is required for their proper folding and cell surface expression (2, 5, 8, 9). MESDC2 is therefore important for cellular Wnt responsiveness (9). When added extracellularly, MESDC2 binds to cell surface LRP6, preventing its interaction with the Wnt antagonist Dkk-1. This binding does not, however, trigger LRP6 internalization or alteration of cytoplasmic beta -catenin levels (8). An LRP5 mutant associated with high bone mass does not interact with MESDC2 (10). MESDC2 itself can be disrupted by a chromosomal translocation occurring in the germ cell tumor, infantile sacrococcygeal teratoma (11).

  1. Holdener, B.C. et al. (1994) Development 120:1335.
  2. Hsieh, J.-C. et al. (2003) Cell 112:355.
  3. Kimelman, D. (2006) Nat. Rev. Genet. 7:360.
  4. Wines, M.E. et al. (2001) Genomics 72:88.
  5. Koduri, V. and S.C. Blacklow (2007) Biochemistry 46:6570.
  6. Culi, J. et al. (2004) EMBO J. 23:1372.
  7. Culi, J. and R.S. Mann (2003) Cell 112:343.
  8. Li, Y. et al. (2005) J. Cell Sci. 118:5305.
  9. Li, Y. et al. (2006) FEBS Lett. 580:5423.
  10. Zhang, Y. et al. (2004) Mol. Cell. Biol. 24:4677.
  11. Veltman, I.M. et al. (2005) Hum. Mol. Genet. 14:1955.
Long Name
Mesoderm Development Candidate 2
Entrez Gene IDs
23184 (Human)
Alternate Names
BOCA; KIAA0081Renal carcinoma antigen NY-REN-61; MESDC2; MESDLDLR chaperone MESD; mesoderm development candidate 2Mesoderm development protein


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse MESDC2 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse MESDC2 Protein, CF and earn rewards!

Have you used Recombinant Mouse MESDC2 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review