Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF

C-Terminal
Catalog # Availability Size / Price Qty
8604-MS-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mesothelin C-Terminal (aa 298-600) His-tag (Catalog 8604-MS) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CA125/MUC16 (Catalog # 5609-MU) that produces 50% optimal binding response is approximately 0.4-2.4 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Mesothelin protein
Asp298-Ser600, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asp298
Predicted Molecular Mass
35 kDa
SDS-PAGE
38-55 kDa, reducing conditions

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8604-MS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8604-MS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Mesothelin

Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF) (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 6). It is up-regulated in mesotheliomas and a range of carcinomas and adenomas (7-10). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression, in part through the up-regulation of MMP-7 (9, 11, 12). It is co-expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (13). A soluble form of Mesothelin, with its GPI anchor intact, is released from tumor cells and binds to MMR/CD206 on macrophages (10, 14-16).

References
  1. Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
  2. Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
  3. Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
  4. Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
  5. Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
  6. Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
  7. Ordonez, N.G. (2003) Mod. Pathol. 16:192.
  8. Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
  9. Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
  10. Scholler, N. et al. (1999) Proc. Natl. Acad. Sci. 96:11531.
  11. Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
  12. Chang, M.-C. et al. (2012) Biochem. J. 442:293.
  13. Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
  14. Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
  15. Robinson, B.W.S. et al. (2003) Lancet 362:1612.
  16. Dangaj, D. et al. (2011) PLoS ONE 6:e28386.
Entrez Gene IDs
10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)
Alternate Names
CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein

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Reviews for Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF

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Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF
By Anonymous on 10/20/2021
Application: In vitro bioactivity in cell culture

Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF
By Anonymous on 05/04/2021
Application: In vitro bioactivity in cell culture

Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF
By Daisy Dai on 01/29/2021
Application: In vitro bioactivity in cell culture