Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF

R&D Systems | Catalog # 8604-MS

C-Terminal
R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Mesothelin (aa 298-600) His Protein (8604-MS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q61468

Applications

Bioactivity
View all Mesothelin Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Mesothelin protein
Asp298-Ser600, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp298

Predicted Molecular Mass

35 kDa

SDS-PAGE

38-55 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Mesothelin C-Terminal (aa 298-600) His-tag (Catalog 8604-MS) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CA125/MUC16 (Catalog # 5609-MU) that produces 50% optimal binding response is approximately 0.4-2.4 ng/mL.

Reviewed Applications

Read 3 reviews rated 5 using 8604-MS in the following applications:

Formulation, Preparation, and Storage

8604-MS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Mesothelin

Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF) (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 6). It is up-regulated in mesotheliomas and a range of carcinomas and adenomas (7-10). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression, in part through the up-regulation of MMP-7 (9, 11, 12). It is co-expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (13). A soluble form of Mesothelin, with its GPI anchor intact, is released from tumor cells and binds to MMR/CD206 on macrophages (10, 14-16).

References

  1. Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
  2. Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
  3. Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
  4. Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
  5. Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
  6. Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
  7. Ordonez, N.G. (2003) Mod. Pathol. 16:192.
  8. Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
  9. Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
  10. Scholler, N. et al. (1999) Proc. Natl. Acad. Sci. 96:11531.
  11. Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
  12. Chang, M.-C. et al. (2012) Biochem. J. 442:293.
  13. Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
  14. Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
  15. Robinson, B.W.S. et al. (2003) Lancet 362:1612.
  16. Dangaj, D. et al. (2011) PLoS ONE 6:e28386.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

Q61468

Additional Mesothelin Products

Product Documents for Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF

Customer Reviews for Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/20/2021
  • Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/04/2021
  • Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF
    Name: Daisy Dai
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/29/2021

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes