Recombinant Mouse MuSK Fc Chimera Protein, CF Summary
Accession # Q61006.1
Complete Your Research
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
In the presence of Recombinant Mouse Agrin, immobilized Recombinant Mouse MuSK Fc Chimera (2 μg/mL at 100 μL/well) binds Recombinant Mouse LRP-4 (10229-LR) with an ED50 of 150-900 ng/mL.
MuSK (muscle-specific kinase) is a 100-kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family (1). It is found in the postsynaptic membrane of skeletal muscle motor endplates (2). MuSK contains a 473 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 353 cytoplasmic domain. Human MuSK has multiple isoforms. One contains deletions at residues 307-394 and 454-461, while a second is a short soluble form that contains residues 120-209 plus a unique 24 aa Cterminal tail (3). Within the ECD, mouse MuSK shares 91% and 96% aa sequence identity with human and rat MuSK, respectively. MuSK is a crucial signaling molecule in the formation of the neuromuscular junction(NMJ). Defects in MuSK causes muscle weakness in congenital myathenic syndromes (4). MuSK binds the heparin sulfate proteoglycan agrin to promote acetylcholine receptor clustering. It has also been found to bind with low-density lipoprotein receptor-related protein 4 (LRP4) (5), Wnt ligands (6), Biglycan (7), ColQ (8) and BMPs(10). Recent studies have showed that MuSK does not bind agrin directly, but enhanced the MuSK-LRP4 interaction (8, 9). When agrin binds to the N-terminal region of LRP4, this promotes the association of LRP4 and MuSK, which then stimulates MuSK kinase activity (11, 12).
- Valenzuela, D.M. et al. (1995) Neuron 15:573.
- Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
- Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
- DeChiara, T. et al. (1996) Cell 85:501.
- Kim, N. et al. (2008) Cell 135:334.
- Banerjee, S. et al. (2011) Development 138:3287.
- Amenta, A.R. et al. (2012) J. Neurosci. 32:2324.
- Otsuka, K. et al. (2015) Scientific Reports 5:13928.
- Zhang, B. et al. (2008) Neuron 60:285.
- Yilmaz, A. et al. (2016) Science Signaling 9:ra87.
- Zhang, W. et al. (2011) J. Biol. Chem. 286:40624.
- Burden, S.J. et al. (2017) CSH Perspectives Biol. 5:a009167.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Mouse MuSK Fc Chimera Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse MuSK Fc Chimera Protein, CF and earn rewards!
Have you used Recombinant Mouse MuSK Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image