Recombinant Mouse LRP-4 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10229-LR-025
Recombinant Mouse LRP-4 His-tag Protein, CF Binding Activity
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Recombinant Mouse LRP-4 His-tag Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse USAG-1 (Catalog # 9008-SD) is present at 2 μg/mL, the concentration of Recombinat Mouse LRP‑4 that produces 50% of the optimal binding response is 80-800 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived mouse LRP-4 protein
Ser21-Ser1725
Accession #
N-terminal Sequence
Analysis
Ser21
Predicted Molecular Mass

192 kDa

SDS-PAGE
198-235 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10229-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Mouse USAG1 (Catalog # 9008-SD) is used at 2 μg/mL (100 μL/well), it binds to Recombinant Mouse LRP-4 (Catalog # 10229-LR) with an ED50 of 80-800 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse LRP-4 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 180-235 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRP-4

LDL receptor-related protein 4 (LRP-4), also known as Megf7, is an approximately 220 kDa type I membrane protein in the LDL receptor superfamily. LRP-4 plays an important role in development of the neuromuscular junction (NMJ), bones, teeth, mammary placodes, and hair follicles (1, 2). Mature mouse LRP-4 consists of a 1705 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 159 aa cytoplasmic domain. The ECD contains 3 EGF-like domains, 8 LDLR-A domains, and 20 LDLR-B repeats that fold into 4 beta-propeller domains (3, 4). Within the ECD, mouse LRP-4 shares 97% and 99% aa sequence identity with human and rat LRP-4, respectively. The ECD of LRP-4 can be shed by ADAM10 mediated cleavage (5). LRP-4 is expressed on myotubes (6, 7), neurons (8, 9), osteocytes and osteoblasts (10, 11), developing tooth epithelium (12), and male and female germ cells (13). It is localized to NMJ on developing myotubes, and it associates in cis with the muscle-specific kinase MuSK (6, 7). Its binding to neuron-derived Agrin promotes additional LRP-4 association with MuSK, MuSK activation, clustering of AChR, and postsynaptic development of the NMJ (6, 7, and 14). LRP-4 also enhances presynaptic differentiation of the NMJ, although this does not require Agrin (14). Neuronal LRP-4 additionally binds ApoE and Fspondin (8, 9). LRP-4 regulates both BMP and Wnt signaling through binding to Wise, Sclerostin, and Dkk1 (10, 12). These interactions are important for the control of bone growth and mineralization, tooth morphogenesis, and the development of mammary placodes, hair follicles, and whiskers (10, 12, and 15).

References
  1. Zong, Y. and R. Jin (2012) Cell. Mol. Life Sci. Nov 22 Epub.
  2. Ohazama, A. et al. (2010) Am. J. Med. Genet. A 152A:2974.
  3. Nakayama, M. et al. (1998) Genomics 51:27.
  4. Tomita, Y. et al. (1998) J. Biochem. 124:784.
  5. Dietrich, M.F. et al. (2010) PLoS ONE 5:e9960.
  6. Kim, N. et al. (2008) Cell 135:334.
  7. Zhang, B. et al. (2008) Neuron 60:285.
  8. Lu, Y. et al. (2007) Brain Res. 1177:19.
  9. Zisman, S. et al. (2007) J. Cell Biol. 178:1237.
  10. Leupin, O. et al. (2011) J. Biol. Chem. 286:19489.
  11. Choi, H.Y. et al. (2009) PLoS ONE 4:e7930.
  12. Ohazama, A. et al. (2008) PLoS ONE 3:e4092.
  13. Yamaguchi, Y.L. et al. (2006) Gene Expr. Patterns 6:607.
  14. Yumoto, N. et al. (2012) Nature 489:438.
  15. Ahn, Y. et al. (2013) Development 140:583.
Long Name
LDL Receptor-related Protein 4
Entrez Gene IDs
4038 (Human); 228357 (Mouse); 83469 (Rat)
Alternate Names
CLSS; KIAA0816; low density lipoprotein receptor-related protein 4; low-density lipoprotein receptor-related protein 4; LRP10; LRP4; LRP-4; MEGF7LRP10; Multiple epidermal growth factor-like domains 7

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