Recombinant Mouse NKG2A Fc Chimera Protein, CF

Datasheet / CoA / SDS
Product Details
Supplemental Products
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse NKG2A/CD159a Fc Chimera is coated at 0.5 µg/mL, recombinant human CD94 binds with a typical ED50 of
    0.1-0.6 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived mouse NKG2A/CD159a protein
    MDPMouse IgG2A
    IEGRMouse NKG2A
    Accession # Q9Z202
  • Accession #
  • N-terminal Sequence
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    44 kDa
    58-67 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Image
When Recombinant Mouse NKG2A Fc Chimera (Catalog # 9198-NK) is coated at 0.5 μg/mL, recombinant human CD94 binds with a typical ED50 of 0.1-0.6 μg/mL.
Background: NKG2A/CD159a
NKG2A/CD159a is an approximately 40 kDa transmembrane C-type lectin superfamily protein that inhibits innate immune system activation (1). Mouse NKG2A consists of a 70 amino acid (aa) cytoplasmic domain with two ITIM inhibitory motifs, a 23 aa transmembrane segment, and a 151 aa extracellular domain (ECD) with one C-type lectin domain (2). Within the ECD, mouse NKG2A shares 41% and 71% aa sequence identity with human and rat NKG2A, respectively. Alternative splicing generates additional isoforms with a 17 aa deletion in the extracellular juxtamembrane region or a substitution of that region plus the transmembrane segment. NKG2A is expressed on a subset of NK cells and CD8+ T cells (2-6) where it forms a covalent heterodimer with CD94 (5, 7, 8). NKG2A-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (2, 7). Triggering the NKG2A-CD94 complex inhibits the cytolytic activity of NK and CD8+ T cells (2, 3, 5, 6, 9). This enables the innate immune system to detect cells that express host MHC-I molecules and to protect them from NK cell mediated lysis. This mechanism is subverted by human cytomegalovirus which encodes a peptide that is homologous to the HLA-E binding peptide (10). HCMV infected cells up-regulate both HLA-E and NKG2A expression and utilize this peptide to escape from immune clearance (3, 10). In contrast, vaccinia virus induces HLA-E down-regulation, thus permitting NK cell lysis of the virally infected cell (11).
  • References:
    1. Das, J. and S.I. Khakoo (2015) Immunol. Rev. 267:214.
    2. Vance, R.E. et al. (1998) J. Exp. Med. 188:1841.
    3. Saez-Borderias, A. et al. (2009) J. Immunol.182:829.
    4. Houchins, J.P. et al. (1997) J. Immunol. 158:3603.
    5. Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
    6. Zhou, J. et al. (2008) J. Immunol. 180:25.
    7. Braud, V.M. et al. (1998) Nature 391:795.
    8. Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
    9. Lee, N. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5199.
    10. Tomasec, P. et al. (2000) Science 287:1031.
    11. Brooks, C.R. et al. (2006) J. Immunol. 176:1141.
  • Long Name:
    Natural Killer G2A
  • Entrez Gene IDs:
    3821 (Human); 16641 (Mouse)
  • Alternate Names:
    CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B
Related Research Areas


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Mouse NKG2A Fc Chimera Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order


Customer Information
You May Also Like

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

R&D Systems Guarantee