When Recombinant Mouse NKG2A Fc Chimera (Catalog # 9198-NK) is coated at 0.5 μg/mL, recombinant human CD94 binds with a typical ED50 of 0.1-0.6 μg/mL.
NKG2A/CD159a is an approximately 40 kDa transmembrane C-type lectin superfamily protein that inhibits innate immune system activation (1). Mouse NKG2A consists of a 70 amino acid (aa) cytoplasmic domain with two ITIM inhibitory motifs, a 23 aa transmembrane segment, and a 151 aa extracellular domain (ECD) with one C-type lectin domain (2). Within the ECD, mouse NKG2A shares 41% and 71% aa sequence identity with human and rat NKG2A, respectively. Alternative splicing generates additional isoforms with a 17 aa deletion in the extracellular juxtamembrane region or a substitution of that region plus the transmembrane segment. NKG2A is expressed on a subset of NK cells and CD8+ T cells (2-6) where it forms a covalent heterodimer with CD94 (5, 7, 8). NKG2A-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (2, 7). Triggering the NKG2A-CD94 complex inhibits the cytolytic activity of NK and CD8+ T cells (2, 3, 5, 6, 9). This enables the innate immune system to detect cells that express host MHC-I molecules and to protect them from NK cell mediated lysis. This mechanism is subverted by human cytomegalovirus which encodes a peptide that is homologous to the HLA-E binding peptide (10). HCMV infected cells up-regulate both HLA-E and NKG2A expression and utilize this peptide to escape from immune clearance (3, 10). In contrast, vaccinia virus induces HLA-E down-regulation, thus permitting NK cell lysis of the virally infected cell (11).
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Natural Killer G2A
Entrez Gene IDs:
3821 (Human); 16641 (Mouse)
CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B
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