Recombinant Mouse Notum Protein, CF

Catalog # Availability Size / Price Qty
9150-NO-050
Recombinant Mouse Notum Protein, CF Bioactivity
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Recombinant Mouse Notum Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.2-1.2  μg/mL in the presence of 0.1 μg/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Mouse myeloma cell line, NS0-derived mouse Notum protein
Ser88-Thr458 (Cys337Ser), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser88, Ser239
Predicted Molecular Mass
43 kDa
SDS-PAGE
39-46 kDa, reducing conditions

Product Datasheets

9150-NO

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse Notum Protein, CF Bioactivity View Larger

RecombinantMouse Wnt-3a shows a dose responsive increase in Wnt reporter activity inHEK293 cells (green line). Recombinant Mouse Notum (Catalog #
9150-NO)inhibits a constant dose of 0.1 µg/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 is 0.2-1.2 µg/mL (orange line).

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Background: Notum

Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity (1, 2). It is tethered to the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions as a Glypican-dependent Wnt inhibitor (1-4). Notum hydrolyzes the palmitoyl moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the Frizzled-8 receptor (1, 2). It also induces the release of cell surface GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR (4). Notum regulates head regeneration in planaria (3), head formation and neural induction in Xenopus (1), and long range activity of hedgehog proteins in Drosophila (5). It is up-regulated in some human hepatocellular carcinomas and colorectal cancers (6, 7). Within amino acids 88-458, mouse Notum shares 92% and 99% aa sequence identity with human and rat Notum, respectively.

References
  1. Zhang, X. et al. (2015) Dev. Cell 32:719.
  2. Kakugawa, S. et al. (2015) Nature 519:187.
  3. Petersen, C.P. and P.W. Reddien (2011) Science 332:852.
  4. Traister, A. et al. (2008) Biochem. J. 410:503.
  5. Ayers, K.L. et al. (2010) Dev. Cell 18:605.
  6. Torisu, Y. et al. (2008) Cancer Sci. 99:1139.
  7. De Robertis, M. et al. (2015) Oncotarget 6:41237.
Entrez Gene IDs
147111 (Human); 77583 (Mouse); 303743 (Rat)
Alternate Names
EC 3.-; notum pectinacetylesterase homolog (Drosophila); Notum; protein notum homolog

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