Recombinant Mouse Notum Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.2-1.2  μg/mL in the presence of 0.1 μg/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
  • Source
    Mouse myeloma cell line, NS0-derived Ser88-Thr458 (Cys337Ser), with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser88, Ser239
  • Predicted Molecular Mass
    43 kDa
  • SDS-PAGE
    39-46 kDa, reducing conditions
9150-NO
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
RecombinantMouse Wnt-3a shows a dose responsive increase in Wnt reporter activity inHEK293 cells (green line). Recombinant Mouse Notum (Catalog #
9150-NO)inhibits a constant dose of 0.1 µg/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 is 0.2-1.2 µg/mL (orange line).
Background: Notum
Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity (1, 2). It is tethered to the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions as a Glypican-dependent Wnt inhibitor (1-4). Notum hydrolyzes the palmitoyl moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the Frizzled-8 receptor (1, 2). It also induces the release of cell surface GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR (4). Notum regulates head regeneration in planaria (3), head formation and neural induction in Xenopus (1), and long range activity of hedgehog proteins in Drosophila (5). It is up-regulated in some human hepatocellular carcinomas and colorectal cancers (6, 7). Within amino acids 88-458, mouse Notum shares 92% and 99% aa sequence identity with human and rat Notum, respectively.
  • References:
    1. Zhang, X. et al. (2015) Dev. Cell 32:719.
    2. Kakugawa, S. et al. (2015) Nature 519:187.
    3. Petersen, C.P. and P.W. Reddien (2011) Science 332:852.
    4. Traister, A. et al. (2008) Biochem. J. 410:503.
    5. Ayers, K.L. et al. (2010) Dev. Cell 18:605.
    6. Torisu, Y. et al. (2008) Cancer Sci. 99:1139.
    7. De Robertis, M. et al. (2015) Oncotarget 6:41237.
  • Entrez Gene IDs:
    147111 (Human); 77583 (Mouse); 303743 (Rat)
  • Alternate Names:
    EC 3.-; notum pectinacetylesterase homolog (Drosophila); Notum; protein notum homolog
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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