Recombinant Mouse Notum Protein, CF Summary
Ser88-Thr458 (Cys337Ser), with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse Wnt-3a shows a dose responsive increase in Wnt reporter activity in HEK293 cells (green line). Recombinant Mouse Notum (Catalog # 9150-NO) inhibits a constant dose of 0.1 µg/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 is 0.2-1.2 µg/mL (orange line).
Notum is an evolutionarily conserved 60 kDa secreted deacylase that regulates Wnt activity (1, 2). It is tethered to the cell surface by binding to Glypican-like sulfated proteoglycans, and it functions as a Glypican-dependent Wnt inhibitor (1-4). Notum hydrolyzes the palmitoyl moiety from Wnt-3a, making the Wnt more hydrophilic and unable to bind to the Frizzled-8 receptor (1, 2). It also induces the release of cell surface GPI-anchored proteins such as Glypican-3, Cadherin-13, and uPAR (4). Notum regulates head regeneration in planaria (3), head formation and neural induction in Xenopus (1), and long range activity of hedgehog proteins in Drosophila (5). It is up-regulated in some human hepatocellular carcinomas and colorectal cancers (6, 7). Within amino acids 88-458, mouse Notum shares 92% and 99% aa sequence identity with human and rat Notum, respectively.
- Zhang, X. et al. (2015) Dev. Cell 32:719.
- Kakugawa, S. et al. (2015) Nature 519:187.
- Petersen, C.P. and P.W. Reddien (2011) Science 332:852.
- Traister, A. et al. (2008) Biochem. J. 410:503.
- Ayers, K.L. et al. (2010) Dev. Cell 18:605.
- Torisu, Y. et al. (2008) Cancer Sci. 99:1139.
- De Robertis, M. et al. (2015) Oncotarget 6:41237.
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