Recombinant Mouse NTB-A/SLAMF6 Fc Chimera Protein, CF Summary
(Glu31 - Asn239)
Accession # Q9ET39
|IEGRMD||Mouse IgG2a |
(Glu98 - Lys330)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
NTB-A, also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature mouse NTB-A consists of a 209 amino acid (aa) ECD with one Ig-like V-type and one Ig-like C2-type domain, a 23 aa transmembrane segment, and an 89 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2). Within the ECD, mouse NTB-A shares 48% and 70% aa sequence identity with human and rat NTB-A, respectively. The ECD of mouse NTB-A shares 20% - 34% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. An alternatively spliced isoform diverges after the second ITSM (2). NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (3 - 5). It interacts homophilically through weak associations between the Ig-V type domains (5 - 7). NTB-A functions as an activating coreceptor on NK and T cells (3, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of NK cell IFN-gamma (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11). The isoform with the divergent C-terminal tail is overexpressed in B cells from lupus-prone mice (11).
- Veillette, A. (2006) Immunol. Rev. 214:22.
- Peck, S.R. and H.E. Ruley (2000) Immunogenetics 52:63.
- Bottino, C. et al. (2001) J. Exp. Med. 194:235.
- Munitz, A. et al. (2005) J. Immunol. 174:110.
- Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.
- Flaig, R.M. et al. (2004) J. Immunol. 172:6524.
- Cao, E. et al. (2006) Immunity 25:559.
- Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
- Eissmann, P. and C. Watzl (2006) J. Immunol. 177:3170.
- Howie, D. et al. (2005) J. Immunol. 174:5931.
- Kumar, K.R. et al. (2006) Science 312:1665.
Citations for Recombinant Mouse NTB-A/SLAMF6 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Invariant NKT Cell Activation Is Potentiated by Homotypic trans-Ly108 Interactions
Authors: Y Baglaenko, M Cruz Tleug, E Gracey, N Talaei, KP Manion, NH Chang, DM Ferri, T Mallevaey, JE Wither
J. Immunol., 2017;0(0):.
Sample Types: Whole Cells
A role for Ly108 in the induction of promyelocytic zinc finger transcription factor in developing thymocytes.
Authors: Dutta, Mala, Kraus, Zachary, Gomez-Rodriguez, Julio, Hwang, Sun-Hee, Cannons, Jennifer, Cheng, Jun, Lee, Sang-Yun, Wiest, David L, Wakeland, Edward K, Schwartzberg, Pamela L
J Immunol, 2013;190(5):2121-8.
Sample Types: Whole Cells
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