Recombinant Mouse Osteopontin/OPN Protein

Carrier Free

Catalog # Availability Size / Price Qty
441-OP-050/CF
441-OP-200/CF

With Carrier

Catalog # Availability Size / Price Qty
441-OP-050
441-OP-200
R&D Systems Recombinant Proteins and Enzymes
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Citations (32)
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Recombinant Mouse Osteopontin/OPN Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261. When 1 x 105 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED50  for this effect is 0.25-1.5 µg/mL. Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE) proteolytic treatment of this rmOsteopontin can increase HEK293 cell adhesion by about 10-fold.
Source
Mouse myeloma cell line, NS0-derived mouse Osteopontin/OPN protein
Leu17-Asn294 (Glu99Gly), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu17
Predicted Molecular Mass
31.5 kDa
SDS-PAGE
30 kDa and a 65 kDa doublet, reducing conditions

Product Datasheets

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441-OP (with carrier)

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441-OP/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

441-OP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

441-OP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Osteopontin/OPN

Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta-1 (early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin-binding ligand N-linked glycoprotein) family of non-collagenous matricellular proteins (1-3). Mouse OPN is synthesized as a 294 amino acid (aa) precursor protein with a 16 aa signal peptide and a 278 aa mature protein (3). Mature mouse OPN shares 79% and 64% aa sequence identity with rat and human OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C‑terminal CD44 binding site (1-4). Depending on tissue-specific modification by O- and N-glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45-75 kDa (5, 6). The central region of OPN contains RGD and non-RGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury
(6-8). OPN can also be cleaved by MMP-3, -7, -9, and -12 within the SLAYGLR motif and at sites closer to the C-terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell ad­hesion and migration (1, 2, 4, 6). Its expression is up-regulat­ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).

References
  1. Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
  2. Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
  3. Miyazaki, Y. et al. (1990) J. Biol. Chem. 265:14432.
  4. Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
  5. Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
  6. Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
  7. Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
  8. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  9. Takafuji, V. et al. (2007) Oncogene 26:6361.
  10. Kiefer, F.W. et al. (2010) Diabetes 59:935.
Long Name
Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin

Citations for Recombinant Mouse Osteopontin/OPN Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. CD248 regulates Wnt signaling in pericytes to promote angiogenesis and tumor growth in lung cancer
    Authors: CL Hong, IS Yu, CH Pai, JS Chen, MS Hsieh, HL Wu, SW Lin, HP Huang
    Cancer Research, 2022;0(0):.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  2. Osteopontin on the Dental Implant Surface Promotes Direct Osteogenesis in Osseointegration
    Authors: S Makishi, T Yamazaki, H Ohshima
    International Journal of Molecular Sciences, 2022;23(3):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  3. Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure
    Authors: FZ Khamissi, L Ning, E Kefaloyian, H Dun, A Arthanaris, A Keller, JJ Atkinson, W Li, B Wong, S Dietmann, K Lavine, D Kreisel, A Herrlich
    Science Advances, 2022;8(8):eabm5900.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Inducible T-Cell Costimulator Ligand Plays a Dual Role in Melanoma Metastasis upon Binding to Osteopontin or Inducible T-Cell Costimulator
    Authors: D Raineri, G Cappellano, B Vilardo, F Maione, N Clemente, E Canciani, E Boggio, CL Gigliotti, C Monge, C Dianzani, R Boldorini, U Dianzani, A Chiocchett
    Biomedicines, 2021;10(1):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Syndecan-4 Protects the Heart From the Profibrotic Effects of Thrombin-Cleaved Osteopontin
    Authors: KM Herum, A Romaine, A Wang, AO Melleby, ME Strand, J Pacheco, B Braathen, P Dunér, T Tønnessen, IG Lunde, I Sjaastad, C Brakebusch, AD McCulloch, MF Gomez, CR Carlson, G Christense
    J Am Heart Assoc, 2020;9(3):e013518.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. The CD153 vaccine is a senotherapeutic option for preventing the accumulation of senescent T cells in mice
    Authors: S Yoshida, H Nakagami, H Hayashi, Y Ikeda, J Sun, A Tenma, H Tomioka, T Kawano, M Shimamura, R Morishita, H Rakugi
    Nat Commun, 2020;11(1):2482.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot Control
  7. The effects of macrophages on cardiomyocyte calcium-handling function using in vitro culture models
    Authors: PG Hitscheric, LH Xie, D Del Re, EJ Lee
    Physiol Rep, 2019;7(13):e14137.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis
    Authors: G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che
    Immunol. Cell Biol., 2018;0(0):.
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  9. Megakaryocyte and Osteoblast Interactions Modulate Bone Mass and Hematopoiesis
    Authors: MB Alvarez, L Xu, P Childress, KA Maupin, SFG Mohamad, B Chitteti, E Himes, DJ Olivos Iii, YH Cheng, SJ Conway, EF Srour, MA Kacena
    Stem Cells Dev., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Bioluminescence imaging visualizes osteopontin-induced neurogenesis and neuroblast migration in the mouse brain after stroke
    Authors: R Rogall, M Rabenstein, S Vay, A Bach, A Pikhovych, J Baermann, M Hoehn, S Couillard-, GR Fink, M Schroeter, MA Rueger
    Stem Cell Res Ther, 2018;9(1):182.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro
    Authors: RM Natoli, H Yu, MC Meislin, P Abbasnia, P Roper, A Vuchkovska, X Xiao, SR Stock, JJ Callaci
    J Orthop Surg Res, 2018;13(1):101.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  12. Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity
    Authors: Q Zeng, X Luo, M Han, W Liu, H Li
    EBioMedicine, 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  13. Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway
    Authors: CH Chen, YM Sue, CY Cheng, YC Chen, CT Liu, YH Hsu, PA Hwang, NJ Huang, TH Chen
    Sci Rep, 2017;7(0):40183.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Intravital F�rster resonance energy transfer imaging reveals osteopontin-mediated polymorphonuclear leukocyte activation by tumor cell emboli
    Authors: Y Kamioka, K Takakura, K Sumiyama, M Matsuda
    Cancer Sci, 2017;108(2):226-235.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  15. Genetic modifiers of muscular dystrophy act on sarcolemmal resealing and recovery from injury
    Authors: M Quattrocel, J Capote, JC Ohiri, JL Warner, AH Vo, JU Earley, M Hadhazy, AR Demonbreun, MJ Spencer, EM McNally
    PLoS Genet., 2017;13(10):e1007070.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. Osteopontin activates mesenchymal stem cells to repair skin wound
    Authors: W Wang, P Li, W Li, J Jiang, Y Cui, S Li, Z Wang
    PLoS ONE, 2017;12(9):e0185346.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Osteopontin facilitates West Nile virus neuroinvasion via neutrophil Trojan horse transport
    Authors: AM Paul, D Acharya, L Duty, EA Thompson, L Le, DS Stokic, AA Leis, F Bai
    Sci Rep, 2017;7(1):4722.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Nanoparticle Tracking Analysis for the Enumeration and Characterization of Mineralo-Organic Nanoparticles in Feline Urine
    PLoS ONE, 2016;11(12):e0166045.
    Species: Feline
    Sample Types: Urine
    Applications: Bioassay
  19. Osteopontin in Spontaneous Germinal Centers Inhibits Apoptotic Cell Engulfment and Promotes Anti-Nuclear Antibody Production in Lupus-Prone Mice
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Early gene activation initiates neuroinflammation prior to VSV neuroinvasion: Impact on antiviral responses and sleep
    Authors: Richard P Ciavarra
    J. Neuroimmunol, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  21. Selective Expression of Osteopontin in ALS-resistant Motor Neurons is a Critical Determinant of Late Phase Neurodegeneration Mediated by Matrix Metalloproteinase-9
    Authors: Yuta Morisaki
    Sci Rep, 2016;6(0):27354.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. 5-Azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival.
    Authors: Maruyama K, Fukasaka M, Uematsu S, Takeuchi O, Kondo T, Saitoh T, Martino M, Akira S
    J Biol Chem, 2015;290(15):9377-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Osteopontin is expressed in the mouse uterus during early pregnancy and promotes mouse blastocyst attachment and invasion in vitro.
    Authors: Qi, Qian-Ron, Xie, Qing-Zhe, Liu, Xue-Li, Zhou, Yun
    PLoS ONE, 2014;9(8):e104955.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  24. An osteopontin-integrin interaction plays a critical role in directing adipogenesis and osteogenesis by mesenchymal stem cells.
    Authors: Chen Q, Shou P, Zhang L, Xu C, Zheng C, Han Y, Li W, Huang Y, Zhang X, Shao C, Roberts A, Rabson A, Ren G, Zhang Y, Wang Y, Denhardt D, Shi Y
    Stem Cells, 2014;32(2):327-37.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Osteopontin expression by CD103- dendritic cells drives intestinal inflammation.
    Authors: Kourepini E, Aggelakopoulou M, Alissafi T, Paschalidis N, Simoes D, Panoutsakopoulou V
    Proc Natl Acad Sci U S A, 2014;111(9):E856-65.
  26. Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
    Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
    J. Exp. Clin. Cancer Res., 2012;31(0):26.
    Species: Human
    Sample Types: Antibody
    Applications: Surface Plasmon Resonance
  27. Osteopontin-Stimulated Expression of Matrix Metalloproteinase-9 Causes Cardiomyopathy in the mdx Model of Duchenne Muscular Dystrophy.
    Authors: Dahiya S, Givvimani S, Bhatnagar S
    J. Immunol., 2011;187(5):2723-31.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  28. Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease.
    Authors: Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR
    FASEB J., 2010;24(1):70-80.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  29. In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression.
    Authors: Marcondes MC, Poling M, Watry DD, Hall D, Fox HS
    Cell. Immunol., 2008;254(1):56-62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  30. Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets.
    Authors: Xanthou G, Alissafi T, Semitekolou M, Simoes DC, Economidou E, Gaga M, Lambrecht BN, Lloyd CM, Panoutsakopoulou V
    Nat. Med., 2007;13(5):570-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  31. Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis.
    Authors: Jacobs JP, Pettit AR, Shinohara ML, Jansson M, Cantor H, Gravallese EM, Mathis D, Benoist C
    Arthritis Rheum., 2004;50(8):2685-94.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  32. Angiotensin II-accelerated atherosclerosis and aneurysm formation is attenuated in osteopontin-deficient mice.
    Authors: Bruemmer D, Collins AR, Noh G, Wang W, Territo M, Arias-Magallona S, Fishbein MC, Blaschke F, Kintscher U, Graf K, Law RE, Hsueh WA
    J. Clin. Invest., 2003;112(9):1318-31.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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Recombinant Mouse Osteopontin/OPN Protein
By Cuiping Zhang on 11/13/2020
Application: In vitro bioactivity in cell culture