Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

R&D Systems | Catalog # 1019-B7

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein (1019-B7)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9EP73

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all PD-L1/B7-H1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse PD-L1/B7-H1 protein
Mouse PD-L1
(Phe19-Thr238)
Accession # Q9EP73		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe19

Predicted Molecular Mass

51.3 kDa (monomer)

SDS-PAGE

75-85 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated mouse T cells.
The ED50 for this effect is 0.15-0.75 µg/mL.

Reviewed Applications

Read 13 reviews rated 5 using 1019-B7 in the following applications:

Scientific Data Images for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein Bioactivity

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein Bioactivity

Recombinant Mouse PD-L1/B7-H1 Fc Chimera (Catalog # 1019-B7) inhibits anti-CD3-induced cell proliferation of stimulated mouse T cells. The ED50 for this effect is 0.15-0.75 μg/mL.
Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein SDS-PAGE

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein SDS-PAGE

1 μg/lane of Recombinant Mouse PD-L1/B7-H1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 79 kDa and 150 kDa, respectively.
Surface plasmon resonance sensorgram of mouse PD-L1 protein binding to mouse PD-1

Binding of Mouse PD-1 to PD-L1/B7-H1 by surface plasmon resonance (SPR).

Recombinant Mouse PD-L1/B7-H1 Fc protein (Catalog # 1019-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Mouse PD-1 His protein (9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM.

Formulation, Preparation, and Storage

1019-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H1 consists of a 221 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, mouse B7-H1 shares 73% and 86% aa sequence identity with human and rat B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (2-5), keratinocytes (6, 7), enothelial and intestinal epithelial cells (6, 8), as well as a variety of carcinomas and melanoma (9, 10). B7-H1 binds to T cell B7-1/CD80 and PD-1 (5, 6, 10-13). It suppresses T cell activation and proliferation (3, 6, 12, 14) and induces the apoptosis of activated T cells (9). It plays a role in the development of immune tolerance by promoting T cell anergy (5, 12) and enhancing regulatory T cell development (14). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (3, 8) and inhibits the development of Th17 cells (14). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (4, 7, 10, 13).

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Tamura, H. et al. (2001) Blood 97:1809.
  3. Chen, L. et al. (2007) J. Immunol. 178:6634.
  4. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  5. Tsushima, F. et al. (2007) Blood 110:180.
  6. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  7. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  8. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  9. Dong, H. et al. (2002) Nat. Med. 8:793.
  10. Azuma, T. et al. (2008) Blood 111:3635.
  11. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  12. Park, J.-J. et al. (2010) Blood 116:1291.
  13. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  14. Herold, M. et al. (2015) J. Immunol. 195:3584.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

Q9EP73

Additional PD-L1/B7-H1 Products

Product Documents for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

Customer Reviews for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF (13)

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  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 02/11/2019
    0.5 ug mPD-L1-hIgG1 (Cat# 1019-B7-100) was added to cells expressing mPD-1 GFP. Binding was detected with anti-human 594 secondary antibody.
    Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF 1019-B7
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 08/26/2018
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 04/12/2018
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 03/30/2018
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 03/15/2018
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 03/11/2018
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 12/18/2017
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/27/2017
    Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF 1019-B7
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/18/2017
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/15/2017
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Shub Poddar
    Application: SDS-PAGE Control
    Verified Customer | Posted 09/08/2017
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 07/20/2017
  • Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SPR experiment
    Verified Customer | Posted 07/14/2017
    SPR experiment for affinity analysis of PDL-1 and Anti PDL-1 Antibody
    Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF 1019-B7

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FAQs for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

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  • Q: Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog # 1019-B7) is a Mouse PD-L1/B7-H1 protein with a human Fc chimera. What is an appropriate isotype control for the Fc?

    A: Recombinant Human IgG1 Fc Protein, CF (Catalog # 110-HG) is an appropriate isotype control for proteins with a human Fc chimera.

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