Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

(6 citations)
(6 Reviews)
  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated mouse T cells. The ED50 for this effect is 0.15-0.75 µg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived
    Mouse PD-L1
    (Phe19-Thr238)
    Accession # Q9EP73
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Phe19
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    51.3 kDa (monomer)
  • SDS-PAGE
    75-85 kDa, reducing conditions
1019-B7
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse PD-L1/B7-H1 Fc Chimera (Catalog # 1019-B7) inhibits anti-CD3-induced cell proliferation of stimulated mouse T cells. The ED50 for this effect is 0.15-0.75 μg/mL.
1 μg/lane of Recombinant Mouse PD‑L1/B7‑H1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 79 kDa and 150 kDa, respectively.
Background: PD-L1/B7-H1
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H1 consists of a 221 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, mouse B7-H1 shares 73% and 86% aa sequence identity with human and rat B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (2-5), keratinocytes (6, 7), enothelial and intestinal epithelial cells (6, 8), as well as a variety of carcinomas and melanoma (9, 10). B7-H1 binds to T cell B7-1/CD80 and PD-1 (5, 6, 10-13). It suppresses T cell activation and proliferation (3, 6, 12, 14) and induces the apoptosis of activated T cells (9). It plays a role in the development of immune tolerance by promoting T cell anergy (5, 12) and enhancing regulatory T cell development (14). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (3, 8) and inhibits the development of Th17 cells (14). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (4, 7, 10, 13).
  • References:
    1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
    2. Tamura, H. et al. (2001) Blood 97:1809.
    3. Chen, L. et al. (2007) J. Immunol. 178:6634.
    4. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
    5. Tsushima, F. et al. (2007) Blood 110:180.
    6. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
    7. Cao, Y. et al. (2010) Cancer Res. 71:1235.
    8. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
    9. Dong, H. et al. (2002) Nat. Med. 8:793.
    10. Azuma, T. et al. (2008) Blood 111:3635.
    11. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
    12. Park, J.-J. et al. (2010) Blood 116:1291.
    13. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
    14. Herold, M. et al. (2015) J. Immunol. 195:3584.
  • Long Name:
    B7 Homolog 1
  • Entrez Gene IDs:
    29126 (Human); 60533 (Mouse); 499342 (Rat); 484186 (Canine); 102145573 (Cynomolgus Monkey)
  • Alternate Names:
    B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Applications
Sample Type
  1. Antibody-drug conjugates bearing pyrrolobenzodiazepine or tubulysin payloads are immunomodulatory and synergize with multiple immunotherapies
    Authors: J Rios-Doria, J Harper, R Rothstein, L Wetzel, J Chesebroug, AM Marrero, C Chen, P Strout, K Mulgrew, KA McGlinchey, R Fleming, B Bezabeh, J Meekin, D Stewart, M Kennedy, P Martin, A Buchanan, N Dimasi, EF Michelotti, RE Hollingswo
    Cancer Res, 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  2. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors
    Authors: H Kinosada, JI Yasunaga, K Shimura, P Miyazato, C Onishi, T Iyoda, K Inaba, M Matsuoka
    PLoS Pathog, 2017;13(1):e1006120.
    Species: N/A
    Sample Type:
  3. Differential control of CD4(+) T-cell subsets by the PD-1/PD-L1 axis in a mouse model of allergic asthma.
    Authors: McAlees J, Lajoie S, Dienger K, Sproles A, Richgels P, Yang Y, Khodoun M, Azuma M, Yagita H, Fulkerson P, Wills-Karp M, Lewkowich I
    Eur J Immunol, 2015;45(4):1019-29.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance.
    Authors: Xiao, Yanping, Yu, Sanhong, Zhu, Baogong, Bedoret, Denis, Bu, Xia, Francisco, Loise M, Hua, Ping, Duke-Cohan, Jonathan, Umetsu, Dale T, Sharpe, Arlene H, DeKruyff, Rosemari, Freeman, Gordon J
    J Exp Med, 2014;211(5):943-59.
    Species: Mouse
    Sample Type: Antibody
    Application: ELISA capture
  5. Soluble production of a biologically active single-chain antibody against murine PD-L1 in Escherichia coli.
    Authors: Drees J, Augustin L, Mertensotto M, Schottel J, Leonard A, Saltzman D
    Protein Expr Purif, 2014;94(0):60-6.
    Species: Mouse
    Sample Type: Antibody
    Application: EnzAct
  6. Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses.
    Authors: Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ
    Immunity, 2007;27(1):111-22.
    Species: N/A
    Sample Type: N/A
    Application: Surface Plasmon Resonance
Recombinant Proteins
Description Application Cat# Citations Images  

Recombinant Human IgG1 Fc, CF

BA 110-HG 66
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We have 6 reviews tested in 3 applications: Binding assay/Protein-protein interaction, SDS-PAGE Control, SPR experiment for affinity analysis of PDL-1 and Anti PDL-1 Antibody .
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Binding assay/Protein-protein interaction Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF 1019-B7
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 Binding assay/Protein-protein interaction Anonymous 11/27/2017
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Binding assay/Protein-protein interaction Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF 1019-B7
Binding assay/Protein-protein interaction: Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF [1019-B7]

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ApplicationBinding assay/Protein-protein interaction
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 Binding assay/Protein-protein interaction Anonymous 11/18/2017
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ApplicationBinding assay/Protein-protein interaction
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 Binding assay/Protein-protein interaction Anonymous 11/15/2017
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 SDS-PAGE Control Anonymous 09/08/2017
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 SDS-PAGE Control Anonymous 07/20/2017
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SPR experiment for affinity analysis of PDL-1 and Anti PDL-1 Antibody  Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF 1019-B7
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 SPR experiment for affinity analysis of PDL-1 and Anti PDL-1 Antibody Anonymous 07/14/2017
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SPR experiment for affinity analysis of PDL-1 and Anti PDL-1 Antibody  Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF 1019-B7
SPR experiment for affinity analysis of PDL-1 and Anti PDL-1 Antibody : Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF [1019-B7]

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ApplicationSPR experiment for affinity analysis of PDL-1 and Anti PDL-1 Antibody

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