Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1019-B7-100
Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF Data
2 Images
Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (13)

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated mouse T cells. The ED50 for this effect is 0.15-0.75 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse PD-L1/B7-H1 protein
Mouse PD-L1
(Phe19-Thr238)
Accession # Q9EP73
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Phe19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51.3 kDa (monomer)
SDS-PAGE
75-85 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1019-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Mouse PD-L1/B7-H1 Fc Chimera (Catalog # 1019-B7) inhibits anti-CD3-induced cell proliferation of stimulated mouse T cells. The ED50for this effect is 0.15-0.75 μg/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse PD-L1/B7-H1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 79 kDa and 150 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H1 consists of a 221 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, mouse B7-H1 shares 73% and 86% aa sequence identity with human and rat B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (2-5), keratinocytes (6, 7), enothelial and intestinal epithelial cells (6, 8), as well as a variety of carcinomas and melanoma (9, 10). B7-H1 binds to T cell B7-1/CD80 and PD-1 (5, 6, 10-13). It suppresses T cell activation and proliferation (3, 6, 12, 14) and induces the apoptosis of activated T cells (9). It plays a role in the development of immune tolerance by promoting T cell anergy (5, 12) and enhancing regulatory T cell development (14). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (3, 8) and inhibits the development of Th17 cells (14). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (4, 7, 10, 13).

References
  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Tamura, H. et al. (2001) Blood 97:1809.
  3. Chen, L. et al. (2007) J. Immunol. 178:6634.
  4. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  5. Tsushima, F. et al. (2007) Blood 110:180.
  6. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  7. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  8. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  9. Dong, H. et al. (2002) Nat. Med. 8:793.
  10. Azuma, T. et al. (2008) Blood 111:3635.
  11. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  12. Park, J.-J. et al. (2010) Blood 116:1291.
  13. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  14. Herold, M. et al. (2015) J. Immunol. 195:3584.
Long Name
Programmed Death Ligand 1
Entrez Gene IDs
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

Citations for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Smad7 Controls Immunoregulatory PDL2/1-PD1 Signaling in Intestinal Inflammation and Autoimmunity
    Authors: LP Garo, AK Ajay, M Fujiwara, V Beynon, C Kuhn, G Gabriely, S Sadhukan, R Raheja, S Rubino, HL Weiner, G Murugaiyan
    Cell Rep, 2019;28(13):3353-3366.e5.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Tolerizing CTL by Sustained Hepatic PD-L1 Expression Provides a New Therapy Approach in Mouse Sepsis
    Authors: A von Knethe, A Schäfer, L Kuchler, T Knape, U Christen, E Hintermann, B Fi beta lthaler, K Schröder, RP Brandes, B Genz, K Abshagen, BM Pützer, LK Sha, A Weigert, SN Syed, M Schulz, AM Shah, A Ernst, M Putyrski, F Finkelmeie, M Pesic, F Greten, M Hogardt, VAJ Kempf, S Gunne, MJ Parnham, B Brüne
    Theranostics, 2019;9(7):2003-2016.
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  3. Improved survival of chimeric antigen receptor-engineered T (CAR-T) and tumor-specific T cells caused by anti-programmed cell death protein 1 single-chain variable fragment-producing CAR-T cells
    Authors: M Nakajima, Y Sakoda, K Adachi, H Nagano, K Tamada
    Cancer Sci., 2019;110(10):3079-3088.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA (Capture)
  4. Intratumoral delivery of an adenoviral vector carrying the SOCS-1 gene enhances T cell-mediated anti-tumor immunity by suppressing PD-L1
    Authors: S Nakagawa, S Serada, R Kakubari, K Hiramatsu, T Sugase, S Matsuzaki, S Matsuzaki, Y Ueda, K Yoshino, T Ohkawara, M Fujimoto, T Kishimoto, T Kimura, T Naka
    Mol. Cancer Ther., 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Combined blockade of IL-6 and PD-1/PD-L1 signaling abrogates mutual regulation of their immunosuppressive effects in the tumor microenvironment
    Authors: H Tsukamoto, K Fujieda, A Miyashita, S Fukushima, T Ikeda, Y Kubo, S Senju, H Ihn, Y Nishimura, H Oshiumi
    Cancer Res., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  6. Indirubin attenuates mouse psoriasis-like skin lesion in a CD274-dependent manner: an achievement of RNA sequencing
    Authors: X Xue, J Wu, J Li, J Xu, H Dai, C Tao, C Li, J Hu
    Biosci. Rep., 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors
    Authors: H Kinosada, JI Yasunaga, K Shimura, P Miyazato, C Onishi, T Iyoda, K Inaba, M Matsuoka
    PLoS Pathog, 2017;13(1):e1006120.
    Species: N/A
    Sample Types:
  8. Antibody-drug conjugates bearing pyrrolobenzodiazepine or tubulysin payloads are immunomodulatory and synergize with multiple immunotherapies
    Authors: J Rios-Doria, J Harper, R Rothstein, L Wetzel, J Chesebroug, AM Marrero, C Chen, P Strout, K Mulgrew, KA McGlinchey, R Fleming, B Bezabeh, J Meekin, D Stewart, M Kennedy, P Martin, A Buchanan, N Dimasi, EF Michelotti, RE Hollingswo
    Cancer Res, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Vectorization in an oncolytic vaccinia virus of an antibody, a Fab and a scFv against programmed cell death -1 (PD-1) allows their intratumoral delivery and an improved tumor-growth inhibition
    Authors: Jean-Baptiste Marchand
    Oncoimmunology, 2016;5(10):e1220467.
    Applications: Bioassay
  10. Obesity accelerates T cell senescence in murine visceral adipose tissue
    Authors: Motoaki Sano
    J. Clin. Invest., 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Differential control of CD4(+) T-cell subsets by the PD-1/PD-L1 axis in a mouse model of allergic asthma.
    Authors: McAlees J, Lajoie S, Dienger K, Sproles A, Richgels P, Yang Y, Khodoun M, Azuma M, Yagita H, Fulkerson P, Wills-Karp M, Lewkowich I
    Eur J Immunol, 2015;45(4):1019-29.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance.
    Authors: Xiao, Yanping, Yu, Sanhong, Zhu, Baogong, Bedoret, Denis, Bu, Xia, Francisco, Loise M, Hua, Ping, Duke-Cohan, Jonathan, Umetsu, Dale T, Sharpe, Arlene H, DeKruyff, Rosemari, Freeman, Gordon J
    J Exp Med, 2014;211(5):943-59.
    Species: Mouse
    Sample Types: Antibody
    Applications: ELISA (Capture)
  13. Soluble production of a biologically active single-chain antibody against murine PD-L1 in Escherichia coli.
    Authors: Drees J, Augustin L, Mertensotto M, Schottel J, Leonard A, Saltzman D
    Protein Expr Purif, 2014;94(0):60-6.
    Species: Mouse
    Sample Types: Antibody
    Applications: Enzyme Assay
  14. Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses.
    Authors: Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ
    Immunity, 2007;27(1):111-22.
    Species: N/A
    Sample Types: N/A
    Applications: Surface Plasmon Resonance

FAQs

  1. The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

    • Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.

  2. What is the recommended method for reconstitution of a lyophilized protein or antibody?

    • Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand.  Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.  

       

  3. Are R&D Systems recombinant proteins and antibodies sterile?

    • Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

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Reviews for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

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Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 02/11/2019
Application: Binding assay/Protein-protein interaction

0.5 ug mPD-L1-hIgG1 (Cat# 1019-B7-100) was added to cells expressing mPD-1 GFP. Binding was detected with anti-human 594 secondary antibody.


Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 08/26/2018
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 04/12/2018
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 03/30/2018
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 03/15/2018
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 03/11/2018
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 12/18/2017
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 11/27/2017
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 11/18/2017
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 11/15/2017
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 09/08/2017
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 07/20/2017
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 07/14/2017
Application: SPR experiment for affinity analysis of PDL-1 and Anti PDL-1 Antibody