Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1019-B7-100
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Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein Bioactivity
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Citations (23)
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Reviews (13)

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated mouse T cells. The ED50 for this effect is 0.15-0.75 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse PD-L1/B7-H1 protein
Mouse PD-L1
(Phe19-Thr238)
Accession # Q9EP73
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Phe19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51.3 kDa (monomer)
SDS-PAGE
75-85 kDa, reducing conditions

Product Datasheets

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1019-B7

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1019-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein Bioactivity View Larger

Recombinant Mouse PD-L1/B7-H1 Fc Chimera (Catalog # 1019-B7) inhibits anti-CD3-induced cell proliferation of stimulated mouse T cells. The ED50 for this effect is 0.15-0.75 μg/mL.

SDS-PAGE Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse PD-L1/B7-H1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 79 kDa and 150 kDa, respectively.

Surface Plasmon Resonance (SPR) Surface plasmon resonance sensorgram of mouse PD-L1 protein binding to mouse PD-1 View Larger

Recombinant Mouse PD-L1/B7-H1 Fc protein (Catalog # 1019-B7) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Mouse PD-1 His protein (9047-PD) was measured at a concentration range between 1.53 nM and 1.56 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.539 uM.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H1 consists of a 221 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, mouse B7-H1 shares 73% and 86% aa sequence identity with human and rat B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (2-5), keratinocytes (6, 7), enothelial and intestinal epithelial cells (6, 8), as well as a variety of carcinomas and melanoma (9, 10). B7-H1 binds to T cell B7-1/CD80 and PD-1 (5, 6, 10-13). It suppresses T cell activation and proliferation (3, 6, 12, 14) and induces the apoptosis of activated T cells (9). It plays a role in the development of immune tolerance by promoting T cell anergy (5, 12) and enhancing regulatory T cell development (14). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (3, 8) and inhibits the development of Th17 cells (14). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (4, 7, 10, 13).

References
  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Tamura, H. et al. (2001) Blood 97:1809.
  3. Chen, L. et al. (2007) J. Immunol. 178:6634.
  4. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  5. Tsushima, F. et al. (2007) Blood 110:180.
  6. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  7. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  8. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  9. Dong, H. et al. (2002) Nat. Med. 8:793.
  10. Azuma, T. et al. (2008) Blood 111:3635.
  11. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  12. Park, J.-J. et al. (2010) Blood 116:1291.
  13. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  14. Herold, M. et al. (2015) J. Immunol. 195:3584.
Long Name
Programmed Death Ligand 1
Entrez Gene IDs
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

Citations for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Upregulation of exosome secretion from tumor-associated macrophages plays a key role in the suppression of anti-tumor immunity
    Authors: Zhong, W;Lu, Y;Han, X;Yang, J;Qin, Z;Zhang, W;Yu, Z;Wu, B;Liu, S;Xu, W;Zheng, C;Schuchter, LM;Karakousis, GC;Mitchell, TC;Amaravadi, R;Flowers, AJ;Gimotty, PA;Xiao, M;Mills, G;Herlyn, M;Dong, H;Mitchell, MJ;Kim, J;Xu, X;Guo, W;
    Cell reports
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Standard
  2. Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8+ T cells
    Authors: Corria-Osorio J, Carmona SJ, Stefanidis E, Andreatta M, Ortiz-Miranda Y, Muller T, Rota IA, Crespo I, Seijo B, Castro W, Jimenez-Luna C, Scarpellino L, Ronet C, Spill A, Lanitis E, Romero P, Luther SA, Irving M, Coukos G
    Nat Immunol. 2023; 24(5): 869–883.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Neuronally expressed PDL1, not PD1, suppresses acute nociception
    Authors: KA Meerschaer, BS Edwards, AY Epouhe, B Jefferson, R Friedman, OL Babyok, JK Moy, F Kehinde, C Liu, CJ Workman, DAA Vignali, KM Albers, HR Koerber, MS Gold, BM Davis, NN Scheff, JL Saloman
    Brain, Behavior, and Immunity, 2022-09-08;106(0):233-246.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells
    Authors: C Martins, M Silva, E Rasbach, P Singh, Y Itoh, JB Williams, E Statham, A Meurer, DV Martinez, A Brandenbur, MV Heppt, SR Barthel, T Schatton
    Scientific Reports, 2022-07-21;12(1):12491.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Computational design of a synthetic PD-1 agonist
    Authors: CM Bryan, GJ Rocklin, MJ Bick, A Ford, S Majri-Morr, AV Kroll, CJ Miller, L Carter, I Goreshnik, A Kang, F DiMaio, KV Tarbell, D Baker
    Proceedings of the National Academy of Sciences of the United States of America, 2021-07-20;118(29):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. PD-L1+ neutrophils contribute to injury-induced infection susceptibility
    Authors: A Thanabalas, AJ Chiang, C Morehouse, M Camara, S Hawkins, AE Keller, AC Koksal, CS Caceres, AA Berlin, N Holoweckyj, VN Takahashi, L Cheng, M de Los Rey, M Pelletier, AC Patera, B Sellman, S Hess, M Marelli, CC Boo, TS Cohen, A DiGiandome
    Science Advances, 2021-03-05;7(10):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  7. ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models
    Authors: A Hanson, K Elpek, E Duong, L Shallberg, M Fan, C Johnson, M Wallace, GR Mabry, S Sazinsky, L Pepper, CJ Shu, S Sathyanara, S Zuerndorfe, T Simpson, M Gostissa, M Briskin, D Law, J Michaelson, CJ Harvey
    PLoS ONE, 2020-09-24;15(9):e0239595.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Quantification of Pharmacokinetic Profiles of PD-1/PD-L1 Antibodies by Validated ELISAs
    Authors: S Zalba, AM Contreras-, E Martisova, R Debets, C Smerdou, MJ Garrido
    Pharmaceutics, 2020-06-26;12(6):.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Capture
  9. Mechanistic dissection of the PD-L1:B7-1 co-inhibitory immune complex
    Authors: SC Garrett-Th, A Massimi, EV Fedorov, JB Bonanno, L Scandiuzzi, B Hillerich, RD Seidel, JD Love, SJ Garforth, C Guha, SC Almo
    PLoS ONE, 2020-06-04;15(6):e0233578.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Smad7 Controls Immunoregulatory PDL2/1-PD1 Signaling in Intestinal Inflammation and Autoimmunity
    Authors: LP Garo, AK Ajay, M Fujiwara, V Beynon, C Kuhn, G Gabriely, S Sadhukan, R Raheja, S Rubino, HL Weiner, G Murugaiyan
    Cell Rep, 2019-09-24;28(13):3353-3366.e5.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Improved survival of chimeric antigen receptor-engineered T (CAR-T) and tumor-specific T cells caused by anti-programmed cell death protein 1 single-chain variable fragment-producing CAR-T cells
    Authors: M Nakajima, Y Sakoda, K Adachi, H Nagano, K Tamada
    Cancer Sci., 2019-09-17;110(10):3079-3088.
    Species: Mouse
    Sample Types: Serum, Whole Cells
    Applications: ELISA (Capture), Flow Cytometry
  12. Tolerizing CTL by Sustained Hepatic PD-L1 Expression Provides a New Therapy Approach in Mouse Sepsis
    Authors: A von Knethe, A Schäfer, L Kuchler, T Knape, U Christen, E Hintermann, B Fi beta lthaler, K Schröder, RP Brandes, B Genz, K Abshagen, BM Pützer, LK Sha, A Weigert, SN Syed, M Schulz, AM Shah, A Ernst, M Putyrski, F Finkelmeie, M Pesic, F Greten, M Hogardt, VAJ Kempf, S Gunne, MJ Parnham, B Brüne
    Theranostics, 2019-03-16;9(7):2003-2016.
    Species: Mouse
    Sample Types: cell culture
    Applications: Bioassay, In Vivo
  13. Indirubin attenuates mouse psoriasis-like skin lesion in a CD274-dependent manner: an achievement of RNA sequencing
    Authors: X Xue, J Wu, J Li, J Xu, H Dai, C Tao, C Li, J Hu
    Biosci. Rep., 2018-11-23;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  14. Combined blockade of IL-6 and PD-1/PD-L1 signaling abrogates mutual regulation of their immunosuppressive effects in the tumor microenvironment
    Authors: H Tsukamoto, K Fujieda, A Miyashita, S Fukushima, T Ikeda, Y Kubo, S Senju, H Ihn, Y Nishimura, H Oshiumi
    Cancer Res., 2018-07-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  15. Intratumoral delivery of an adenoviral vector carrying the SOCS-1 gene enhances T cell-mediated anti-tumor immunity by suppressing PD-L1
    Authors: S Nakagawa, S Serada, R Kakubari, K Hiramatsu, T Sugase, S Matsuzaki, S Matsuzaki, Y Ueda, K Yoshino, T Ohkawara, M Fujimoto, T Kishimoto, T Kimura, T Naka
    Mol. Cancer Ther., 2018-06-11;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. Antibody-drug conjugates bearing pyrrolobenzodiazepine or tubulysin payloads are immunomodulatory and synergize with multiple immunotherapies
    Authors: J Rios-Doria, J Harper, R Rothstein, L Wetzel, J Chesebroug, AM Marrero, C Chen, P Strout, K Mulgrew, KA McGlinchey, R Fleming, B Bezabeh, J Meekin, D Stewart, M Kennedy, P Martin, A Buchanan, N Dimasi, EF Michelotti, RE Hollingswo
    Cancer Res, 2017-03-10;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors
    Authors: H Kinosada, JI Yasunaga, K Shimura, P Miyazato, C Onishi, T Iyoda, K Inaba, M Matsuoka
    PLoS Pathog, 2017-01-03;13(1):e1006120.
  18. Obesity accelerates T cell senescence in murine visceral adipose tissue
    Authors: Motoaki Sano
    J. Clin. Invest., 2016-11-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Vectorization in an oncolytic vaccinia virus of an antibody, a Fab and a scFv against programmed cell death -1 (PD-1) allows their intratumoral delivery and an improved tumor-growth inhibition
    Authors: Jean-Baptiste Marchand
    Oncoimmunology, 2016-09-09;5(10):e1220467.
    Species: Mouse
    Sample Types: Protein, Whole Cells
    Applications: Bioassay
  20. Differential control of CD4(+) T-cell subsets by the PD-1/PD-L1 axis in a mouse model of allergic asthma.
    Authors: McAlees J, Lajoie S, Dienger K, Sproles A, Richgels P, Yang Y, Khodoun M, Azuma M, Yagita H, Fulkerson P, Wills-Karp M, Lewkowich I
    Eur J Immunol, 2015-02-26;45(4):1019-29.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance.
    Authors: Xiao, Yanping, Yu, Sanhong, Zhu, Baogong, Bedoret, Denis, Bu, Xia, Francisco, Loise M, Hua, Ping, Duke-Cohan, Jonathan, Umetsu, Dale T, Sharpe, Arlene H, DeKruyff, Rosemari, Freeman, Gordon J
    J Exp Med, 2014-04-21;211(5):943-59.
    Species: Mouse
    Sample Types: Antibody
    Applications: ELISA (Capture)
  22. Soluble production of a biologically active single-chain antibody against murine PD-L1 in Escherichia coli.
    Authors: Drees J, Augustin L, Mertensotto M, Schottel J, Leonard A, Saltzman D
    Protein Expr Purif, 2013-11-08;94(0):60-6.
    Species: Mouse
    Sample Types: Antibody, Whole Cells
    Applications: Bioassay, Enzyme Assay
  23. Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses.
    Authors: Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ
    Immunity, 2007-07-12;27(1):111-22.
    Applications: Surface Plasmon Resonance

FAQs

  1. Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog # 1019-B7) is a Mouse PD-L1/B7-H1 protein with a human Fc chimera. What is an appropriate isotype control for the Fc?

    • Recombinant Human IgG1 Fc Protein, CF (Catalog # 110-HG) is an appropriate isotype control for proteins with a human Fc chimera.

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Reviews for Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF

Average Rating: 5 (Based on 13 Reviews)

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Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 02/11/2019
Application: Binding assay/Protein-protein interaction

0.5 ug mPD-L1-hIgG1 (Cat# 1019-B7-100) was added to cells expressing mPD-1 GFP. Binding was detected with anti-human 594 secondary antibody.


Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 08/26/2018
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 04/12/2018
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 03/30/2018
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 03/15/2018
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 03/11/2018
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 12/18/2017
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 11/27/2017
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 11/18/2017
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 11/15/2017
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Shub Poddar on 09/08/2017
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 07/20/2017
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein, CF
By Anonymous on 07/14/2017
Application: SPR experiment