Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF

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  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated mouse T cells. The ED50 for this effect is 1-6 µg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived mouse PD-L2/B7-DC protein
    Mouse PD-L2
    (Leu20-Arg219)
    Accession # Q9WUL5
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu20
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    49 kDa (monomer)
  • SDS-PAGE
    76 kDa, reducing conditions
Product Datasheets

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1022-PL
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 400 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
Recombinant Mouse PD-L2/B7-DC Fc Chimera (Catalog # 1022-PL)inhibits anti-CD3 induced T cell proliferation. The ED50 for this effect is1-6 μg/mL.
Background: PD-L2/B7-DC
Programmed Death Ligand 2 (PD-L2), also known as B7-DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Mature mouse PD-L2 consists of a 199 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 23 aa transmembrane segment, and a 5 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2 share 72% aa sequence identity. PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3-5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10).
PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). In asthma, PD-L2 suppresses IL-5 and
IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).
  • References:
    1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
    2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
    3. Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
    4. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
    5. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
    6. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
    7. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
    8. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
    9. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
    10. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
    11. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
    12. Shin, T. et al. (2003) J. Exp. Med. 198:31.
    13. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.
  • Long Name:
    Programmed Death-1 Ligand-2
  • Entrez Gene IDs:
    80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)
  • Alternate Names:
    B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; Butyrophilin-like Protein; CD273 antigen; CD273; CD273PD-1 ligand 2; MGC142240; PD-1-ligand 2; PDCD1L2MGC142238; PDCD1LG2; PDL2; PD-L2; PDL2B7DC; PD-L2PDCD1 ligand 2; programmed cell death 1 ligand 2; Programmed death ligand 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance.
    Authors: Xiao, Yanping, Yu, Sanhong, Zhu, Baogong, Bedoret, Denis, Bu, Xia, Francisco, Loise M, Hua, Ping, Duke-Cohan, Jonathan, Umetsu, Dale T, Sharpe, Arlene H, DeKruyff, Rosemari, Freeman, Gordon J
    J Exp Med, 2014;211(5):943-59.
    Species: Mouse
    Sample Type: Antibody
    Application: ELISA capture
  2. Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses.
    Authors: Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ
    Immunity, 2007;27(1):111-22.
    Species: N/A
    Sample Type: N/A
    Application: Surface Plasmon Resonance

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Reconstitution Buffer 1 (PBS)

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