Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF Summary
Accession # Q9WUL5
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 400 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse PD-L2/B7-DC Fc Chimera (Catalog # 1022-PL) inhibits anti-CD3 induced T cell proliferation. The ED50 for this effect is 1-6 μg/mL.
Programmed Death Ligand 2 (PD-L2), also known as B7-DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Mature mouse PD-L2 consists of a 199 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 23 aa transmembrane segment, and a 5 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2 share 72% aa sequence identity. PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3-5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10).
PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). In asthma, PD-L2 suppresses IL-5 and
IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).
- Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
- Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
- Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
- Messal, N. et al. (2011) Mol. Immunol. 48:2214.
- Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
- Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
- Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
- Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
- Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
- Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
- Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
- Shin, T. et al. (2003) J. Exp. Med. 198:31.
- Xiao, Y. et al. (2014) J. Exp. Med. 211:943.
Citations for Recombinant Mouse PD-L2/B7-DC Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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PD-L2 controls peripherally induced regulatory T cells by maintaining metabolic activity and Foxp3 stability
Authors: BP Hurrell, DG Helou, E Howard, JD Painter, P Shafiei-Ja, AH Sharpe, O Akbari
Nature Communications, 2022;13(1):5118.
Sample Types: Whole Cells
Smad7 Controls Immunoregulatory PDL2/1-PD1 Signaling in Intestinal Inflammation and Autoimmunity
Authors: LP Garo, AK Ajay, M Fujiwara, V Beynon, C Kuhn, G Gabriely, S Sadhukan, R Raheja, S Rubino, HL Weiner, G Murugaiyan
Cell Rep, 2019;28(13):3353-3366.e5.
Sample Types: In Vivo
Applications: In Vivo
RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance.
Authors: Xiao, Yanping, Yu, Sanhong, Zhu, Baogong, Bedoret, Denis, Bu, Xia, Francisco, Loise M, Hua, Ping, Duke-Cohan, Jonathan, Umetsu, Dale T, Sharpe, Arlene H, DeKruyff, Rosemari, Freeman, Gordon J
J Exp Med, 2014;211(5):943-59.
Sample Types: Antibody
Applications: ELISA (Capture)
Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses.
Authors: Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ
Sample Types: N/A
Applications: Surface Plasmon Resonance
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