Recombinant Mouse PD-L2/B7-DC (K113S) Fc Chimera Protein, CF Summary
Accession # Q9WUL5
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
When Recombinant Mouse PD‑L2/B7‑DC (K113S) FcChimera (Catalog # 10021-PL) is immobilized at 2 µg/mL,Recombinant Mouse RGMB (Catalog # 3597-RG) binds with an ED50 of 0.08-0.48 μg/mL.
2 μg/lane of Recombinant Mouse PD‑L2/B7‑DC (K113S) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 64-84 kDa and 125-170 kDa, respectively.
Programmed Death Ligand 2 (PD-L2), also known as Butyrophilin B7-DC, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). In humans, the mature PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2, 3). Within the ECD, mouse PD-L2 shares 72% and 95% aa sequence identity with human and rat PD‑L2, respectively. PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3‑5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to repulsive guidance molecule family member b (RGMb) on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). Replacement of lysine residue at position 113 with serine (K113S) has been reported to result in a loss of binding capacity to PD-1, while retaining its T-cell stimulatory function through its interaction with RGMb (14). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).
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