Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse LDLR (Catalog # 2255-LD) is coated at 2 µg/mL, Recombinant Mouse Proprotein Convertase 9/PCSK9 (Catalog #
    9258-SE) binds with an ED50 = 25-150 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Ser156-Gln694 with a C-terminal 10-His tag; Gln35-Gln155
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser156 (mature form); No results obtained for pro domain, Gln35 inferred from enzymatic pyroglutamate treatment revealing Asp36
  • Predicted Molecular Mass
    14 (pro domain) and 59 (mature form) kDa
  • SDS-PAGE
    18 kDa (pro domain) and 57-67 kDa (mature form), reducing conditions 
9258-SE
 
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Reconstitution


Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Data Images
Recombinant Mouse Proprotein Convertase 9/
PCSK9 Binds toRecombinant Mouse LDL R in an ELISA Binding Assay. When Recombinant Mouse LDL R Recombinant Mouse LDL R (Catalog # 2255-LD) is coated at 2 µg/mL, Recombinant Mouse Proprotein Convertase 9/PCSK9 (Catalog # 9258-SE) binds with an
ED50 = 25-150 ng/mL.
Background: Proprotein Convertase 9/PCSK9
PCSK9 (proprotein convertase subtilisin kexin 9), also known as NARC-1, is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. It is highly expressed in the liver, intestine, and kidney and plays an important role in regulating LDL R expression and circulating cholesterol levels (1). PCSK9 is synthesized as precursor protein that is autocatalytically cleaved in the endoplasmic reticulum to generate a 14 kDa prodomain and a 60 kDa catalytic domain (2). Within the secretion pathway, the prodomain remains associated with and functions as a chaperone for the catalytic domain. Mouse PCSK9 shares 78% and 93% amino acid identity with human and rat PCSK9, respectively. PCSK9 plays a key role in the regulation of cholesterol metabolism by binding to hepatic LDL R, LRP-1, VLDL R, and Apolipoprotein E R2 and promoting their lysosomal degradation instead of recycling to the plasma membrane (3-8). It can also regulate cholesterol and triglyceride handling in the intestine and adipose tissue (9-11). The ability of PCSK9 to regulate LDL R expression is inhibited by its binding to LDL particles or Annexin A2 or by additional proteolytic cleavage (12-17).
  • References:
    1. Schulz, R. et al. (2015) Basic Res. Cardiol. 110:4.
    2. Benjannet, S. et al. (2004) J. Biol. Chem. 279:48865.
    3. Lagace, T.A. et al. (2006) J. Clin. Invest. 116:2995.
    4. Grefhorst, A. et al. (2008) J. Lipid Res. 49:1303.
    5. Zhang, D.W. (2007) J. Biol. Chem. 282:18602.
    6. DeVay, R.M. et al. (2013) J. Biol. Chem. 288:10805.
    7. Canuel, M. et al. (2013) PLoS One 8:e64145.
    8. Poirier, S. et al. (2008) J. Biol. Chem. 283:2363.
    9. Levy, E. et al. (2013) Atherosclerosis 227:297.
    10. Le May, C. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:1484.
    11. Roubtsova, A. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:785.
    12. Kosenko, T. et al. (2013) J. Biol. Chem. 288:8279.
    13. Mayer, G. et al. (2008) J. Biol. Chem. 283:31791.
    14. Benjannet, S. et al. (2006) J. Biol. Chem. 281:30561.
    15. Benjannet, S. et al. (2010) J. Biol. Chem. 285:40965.
    16. Essalmani, R. et al. (2011) J. Biol. Chem. 286:4257.
    17. Han, B. et al. (2014) J. Lipid Res. 55:1505.
  • Entrez Gene IDs:
    255738 (Human); 100102 (Mouse); 298296 (Rat)
  • Alternate Names:
    EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein convertase 9; Proprotein Convertase 9/PCSK9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP MAB050 17  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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