Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein, CF

Catalog # Availability Size / Price Qty
9258-SE-020
Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein Bioactivity
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Citations (2)
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Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse LDLR (Catalog # 2255-LD) is coated at 2 µg/mL, Recombinant Mouse Proprotein Convertase 9/PCSK9 (Catalog #
9258-SE) binds with an ED50 = 25-150 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Proprotein Convertase 9/PCSK9 protein
Ser156-Gln694 with a C-terminal 10-His tag; Gln35-Gln155
Accession #
N-terminal Sequence
Analysis
Ser156 (mature form); No results obtained for pro domain, Gln35 inferred from enzymatic pyroglutamate treatment revealing Asp36
Structure / Form
Pro domain & mature form
Predicted Molecular Mass
14 kDa (pro domain) and 59 kDa (mature form)
SDS-PAGE
18 kDa (pro domain) and 57-67 kDa (mature form), reducing conditions 

Product Datasheets

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9258-SE

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9258-SE

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:


Scientific Data

Bioactivity Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein Bioactivity View Larger

Recombinant Mouse Proprotein Convertase 9/ PCSK9 Binds to Recombinant Mouse LDL R in an ELISA Binding Assay. When Recombinant Mouse LDL R Recombinant Mouse LDL R (Catalog # 2255-LD) is coated at 2 µg/mL, Recombinant Mouse Proprotein Convertase 9/PCSK9 (Catalog # 9258-SE) binds with an ED50 = 25-150 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: Proprotein Convertase 9/PCSK9

PCSK9 (proprotein convertase subtilisin kexin 9), also known as NARC-1, is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. It is highly expressed in the liver, intestine, and kidney and plays an important role in regulating LDL R expression and circulating cholesterol levels (1). PCSK9 is synthesized as precursor protein that is autocatalytically cleaved in the endoplasmic reticulum to generate a 14 kDa prodomain and a 60 kDa catalytic domain (2). Within the secretion pathway, the prodomain remains associated with and functions as a chaperone for the catalytic domain. Mouse PCSK9 shares 78% and 93% amino acid identity with human and rat PCSK9, respectively. PCSK9 plays a key role in the regulation of cholesterol metabolism by binding to hepatic LDL R, LRP-1, VLDL R, and Apolipoprotein E R2 and promoting their lysosomal degradation instead of recycling to the plasma membrane (3-8). It can also regulate cholesterol and triglyceride handling in the intestine and adipose tissue (9-11). The ability of PCSK9 to regulate LDL R expression is inhibited by its binding to LDL particles or Annexin A2 or by additional proteolytic cleavage (12-17).

References
  1. Schulz, R. et al. (2015) Basic Res. Cardiol. 110:4.
  2. Benjannet, S. et al. (2004) J. Biol. Chem. 279:48865.
  3. Lagace, T.A. et al. (2006) J. Clin. Invest. 116:2995.
  4. Grefhorst, A. et al. (2008) J. Lipid Res. 49:1303.
  5. Zhang, D.W. (2007) J. Biol. Chem. 282:18602.
  6. DeVay, R.M. et al. (2013) J. Biol. Chem. 288:10805.
  7. Canuel, M. et al. (2013) PLoS One 8:e64145.
  8. Poirier, S. et al. (2008) J. Biol. Chem. 283:2363.
  9. Levy, E. et al. (2013) Atherosclerosis 227:297.
  10. Le May, C. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:1484.
  11. Roubtsova, A. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:785.
  12. Kosenko, T. et al. (2013) J. Biol. Chem. 288:8279.
  13. Mayer, G. et al. (2008) J. Biol. Chem. 283:31791.
  14. Benjannet, S. et al. (2006) J. Biol. Chem. 281:30561.
  15. Benjannet, S. et al. (2010) J. Biol. Chem. 285:40965.
  16. Essalmani, R. et al. (2011) J. Biol. Chem. 286:4257.
  17. Han, B. et al. (2014) J. Lipid Res. 55:1505.
Entrez Gene IDs
255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)
Alternate Names
EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; FHCL3; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9

Citations for Recombinant Mouse Proprotein Convertase 9/PCSK9 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Rosa26-LSL-dCas9-VPR: a versatile mouse model for tissue specific and simultaneous activation of multiple genes for drug discovery
    Authors: D Pakalnišky, T Schönberge, B Strobel, B Stierstorf, T Lamla, M Schuler, M Lenter
    Scientific Reports, 2022;12(1):19268.
    Species: N/A
    Sample Types: Buffer
    Applications: Western Blot
  2. All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo
    Authors: R Ibraheim, CQ Song, A Mir, N Amrani, W Xue, EJ Sontheimer
    Genome Biol., 2018;19(1):137.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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