Serpin E2, also known as protease nexin I or glial-derived nexin (GDN), is a member of the Serpin superfamily of the serine protease inhibitors (1). Serpin E2 is a potent inhibitor of thrombin, plasmin and plasminogen activators (2). It is differentially expressed during neuronal differentiation and is able to transform human embryonic kidney cells into neuron-like cells (3). Its over‑expression in mice leads to progressive neuronal and motor dysfunction in these animals (4). It is also over‑expressed in the majority of pancreatic carcinoma as well as gastric and colorectal cancer samples whereas it is weakly expressed in all normal pancreas and chronic pancreatitis tissue samples (5). It plays an important role in controlling male fertility because its knockout male mice show a marked impairment in fertility from the onset of sexual maturity and its abnormal expression is found in the semen of men with seminal dysfunction (6).
Recombinant Mouse Serpin E2/PN1 Protein, CF
R&D Systems | Catalog # 2175-PI
Key Product Details
- R&D Systems NS0-derived Recombinant Mouse Serpin E2/PN1 Protein (2175-PI)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Ser20-Pro397, with a C-terminal 10-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <2 nM, as measured under the described conditions.
Formulation, Preparation, and Storage
2175-PI
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Serpin E2/PN1
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Rossignol, P. et al. (2004) J. Biol. Chem. 279:10346.
- Lin, H.J. et al. (2005) Int. J. Dev. Neurosci. 23:9.
- Meins, M. et al. (2001) J. Neurosci. 21:8830.
- Buchholz, M. et al. (2003) Cancer Res. 63:4945.
- Murer, V. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3029.
Alternate Names
Gene Symbol
UniProt
Additional Serpin E2/PN1 Products
Product Documents for Recombinant Mouse Serpin E2/PN1 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse Serpin E2/PN1 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Mouse Serpin E2/PN1 Protein, CF
Powered by Bioz
Customer Reviews for Recombinant Mouse Serpin E2/PN1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse Serpin E2/PN1 Protein, CF and earn rewards!
Have you used Recombinant Mouse Serpin E2/PN1 Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
View specific protocols for Recombinant Mouse Serpin E2/PN1 Protein, CF (2175-PI):
- Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5 (TCNB)
- Recombinant Mouse Serpin E2/PN1 (rmSerpin E2) (Catalog # 2175-PI)
- Trypsin (Sigma, Catalog # T-1426)
- Substrate: MCA-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2 (Catalog # ES002)
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute Trypsin to 0.25 μg/mL in Assay Buffer and KEEP ON ICE.
- Prepare a curve of rmSerpin E2 (MW: 43,200 Da) in Assay Buffer. Make the following serial dilutions: 500, 100, 50, 30, 20, 10, 5, 2.5, and 0.625 nM.
- Combine equal volumes of diluted Trypsin and rmSerpin E2 at each concentration of the curve. Include two controls containing equal volumes of Assay Buffer and diluted Trypsin.
- Incubate at room temperature for 30 minutes.
- Dilute reaction mixtures five fold in Assay Buffer.
- Dilute Substrate to 20 μM in Assay Buffer.
- Load 50 μL of the diluted mixtures in a plate, and start the reaction by adding 50 μL of 20 μM Substrate to wells.
- Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
- Derive the 50% inhibiting concentration (IC50) of rmSerpin E2 by plotting RFU/min (or specific activity) vs. concentration with 4-PL fitting.
- The specific activity for Trypsin at each point may be determined using the following formula (if needed):
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Lys-OH (Bachem, Catalog # M-1975).
Per Well:
- Trypsin: 0.00125 μg
- rmSerpin E2 curve: 25, 5, 2.5, 1.5, 1, 0.5, 0.25, 0.125, and 0.03125 nM
- Substrate: 10 μM