Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate

Substrate for MMP-3, MMP-10, Trypsin, HGF Activator, and Coagulation Factor X
Catalog # Availability Size / Price Qty
ES002
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)

Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate Summary

Purity
>95%, by HPLC.
Activity
The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence. It is an excellent substrate for MMP-3 (stromelysin 1) and MMP-10 (stromelysin 2). The cleavage site is the peptide bond between Glu and Nval (norvaline).

It is also an excellent substrate for Trypsin, HGF activator and factor Xa.

Source

Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2
Mca: (7-Methoxycoumarin-4-yl)acetyl, Nval: Norvaline, Dnp: 2, 4-Dinitrophenyl.
Predicted Molecular Mass
1676 Da

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ES002

Formulation Supplied as a stock solution in dimethyl sulfoxide (DMSO) at a concentration of 10.0 mg/mL.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Citations for Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Protease-inhibitor interaction predictions: Lessons on the complexity of protein-protein interactions
    Authors: N Fortelny, GS Butler, CM Overall, P Pavlidis
    Mol. Cell Proteomics, 2017;0(0):.
    Applications: Bioassay
  2. Inhibition of Human Kallikrein 5 Protease by Triterpenoids from Natural Sources
    Authors: Y Matsubara, T Matsumoto, J Koseki, A Kaneko, S Aiba, K Yamasaki
    Molecules, 2017;22(11):.
  3. Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stage.
    Authors: Zhao L, Hua T, Crowley C, Ru H, Ni X, Shaw N, Jiao L, Ding W, Qu L, Hung L, Huang W, Liu L, Ye K, Ouyang S, Cheng G, Liu Z
    Cell Res, 2014;24(3):344-58.
    Species: Mouse
    Sample Types: Protein
  4. Proteolytic activation cascade of the Netherton syndrome-defective protein, LEKTI, in the epidermis: implications for skin homeostasis.
    Authors: Fortugno P, Bresciani A, Paolini C, Pazzagli C, El Hachem M, D'Alessio M, Zambruno G
    J Invest Dermatol, 2011;131(11):2223-32.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  5. The extracellular matrix protein fibronectin is a substrate for kallikrein 7.
    Authors: Ramani VC, Haun RS
    Biochem. Biophys. Res. Commun., 2008;369(4):1169-73.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. Crystal structure of human epidermal kallikrein 7 (hK7) synthesized directly in its native state in E. coli: insights into the atomic basis of its inhibition by LEKTI domain 6 (LD6).
    Authors: Fernandez IS, Standker L, Magert HJ, Forssmann WG, Gimenez-Gallego G, Romero A
    J. Mol. Biol., 2008;377(5):1488-97.
    Species: Human
    Sample Types:
    Applications: Bioassay
  7. Autosomal ichthyosis with hypotrichosis syndrome displays low matriptase proteolytic activity and is phenocopied in ST14 hypomorphic mice.
    Authors: List K, Currie B, Scharschmidt TC, Szabo R, Shireman J, Molinolo A, Cravatt BF, Segre J, Bugge TH
    J. Biol. Chem., 2007;282(50):36714-23.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  8. Microparticles of human atherosclerotic plaques enhance the shedding of the tumor necrosis factor-alpha converting enzyme/ADAM17 substrates, tumor necrosis factor and tumor necrosis factor receptor-1.
    Authors: Canault M, Leroyer AS, Peiretti F, Leseche G, Tedgui A, Bonardo B, Alessi MC, Boulanger CM, Nalbone G
    Am. J. Pathol., 2007;171(5):1713-23.
    Species: Human
    Sample Types: Biofilm
    Applications: Bioassay
  9. Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
    Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
    Cancer Cell, 2006;10(1):39-50.
    Species: Human
    Sample Types: Protein
  10. Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
    Authors: Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA
    Genes Dev., 2006;20(5):543-56.
    Species: Human
    Sample Types: Buffer

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Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate
By Anonymous on 08/10/2017
Application: Enzymatic activity in vitro