Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate

R&D Systems | Catalog # ES002

Substrate for MMP-3, MMP-10, Trypsin, HGF Activator, and Coagulation Factor X
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Key Product Details

Conjugate

7-Methoxycoumarin

Applications

Binding Activity, Bioactivity
View all Protease Substrates Fluorescent Probes and Dyes »

Product Specifications

Source


Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2
Mca: (7-Methoxycoumarin-4-yl)acetyl, Nval: Norvaline, Dnp: 2, 4-Dinitrophenyl.

Purity

>95%, by HPLC.

Predicted Molecular Mass

1676 Da

Activity

The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence.

It is an excellent substrate for MMP-3 (stromelysin 1) and MMP-10 (stromelysin 2). The cleavage site is the peptide bond between Glu and Nval (norvaline).

It is also an excellent substrate for Trypsin, HGF activator and factor Xa.

Reviewed Applications

Read 3 reviews rated 5 using ES002 in the following applications:

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Formulation, Preparation, and Storage

ES002
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.

Product Documents for Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate

For research use only

Citations for Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate

Customer Reviews for Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate (3)

5 out of 5
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  • Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 07/08/2024
    Fluorescent signal of KLK7 enzyme in the absence or in presence of MMP fluorogenic substrate.
    We used this substrate to evaluate KLK7 activity in tubo.
    Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate ES002
  • Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 03/13/2020
    Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate ES002
  • Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 08/10/2017

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