Recombinant Mouse SF20/MYDGF Protein, CF

R&D Systems | Catalog # 10232-MY

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse SF20/MYDGF Protein (10232-MY)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9CPT4

Applications

Bioactivity
View all SF20/MYDGF Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SF20/MYDGF protein
Val25-Leu166

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val25

Predicted Molecular Mass

16 kDa

SDS-PAGE

15-16 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse SF20/MYDGF (Catalog # 10232-MY) is immobilized at 2 μg/mL (100 μL/well), Recombinant Human Protein Disulfide Isomerase/P4HB (Catalog # 4236-DI) binds with an ED50 of 0.7-4.2 μg/mL.

Scientific Data Images for Recombinant Mouse SF20/MYDGF Protein, CF

Recombinant Mouse SF20/MYDGF Protein Binding Activity

Recombinant Mouse SF20/MYDGF Protein Binding Activity

When Recombinant Mouse SF20/MYDGF (Catalog # 10232-MY) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Protein Disulfide Isomerase/P4HB (Catalog # 4236-DI) binds with an ED50 of 0.7-4.2 µg/mL.
Recombinant Mouse SF20/MYDGF Protein SDS-PAGE

Recombinant Mouse SF20/MYDGF Protein SDS-PAGE

2 μg/lane of Recombinant Mouse SF20/MYDGF (Catalog # 10232-MY) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 15-16 kDa.

Formulation, Preparation, and Storage

10232-MY
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SF20/MYDGF

Myeloid-Derived Growth Factor, or MYDGF, is a Bone marrow-derived monocyte protein, and it is correlated with enhanced metabolic activity, suppression of apoptosis, and stimulation of cell proliferation (1). MYDGF is expressed predominantly in inflammatory cells, such as monocytes and macrophages (1). Up-regulation of MYDGF expression was also found during adipocyte differentiation (2). Expression of MYDGF was induced in the circulation and heart tissue after myocardial infarction. It promotes cardiac myocyte survival by stimulating endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway, and inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (1). MYDGF was found over-expressed in approximately two-thirds of Hepatocellular Carcinoma (HCC) tissues, and its expression was significantly positively correlated with that of alpha-fetoprotein (AFP) (3). In HCC, MYDGF could regulate cell proliferation through activating Akt/mitogen-activated protein kinase pathways (3). Mouse MYDGF shares 92% amino acid sequence identity with both human and rat MYDGF. Intriguingly, virtually all homologs of MYDGF have a C-terminal putative ER retention sequence BXEL (B: Arg, His, or Lys; X: variable residue; E: Glu; L: Leu), which has the potential to retain human MYDGF and its homologs in the ER, whereas truncated MYDGF without BXEL is secreted from the cell (4). However, the functions of these different forms remain unclear.

References

  1. Korf-Klingebiel, M. et al. (2015) Nat. Med. 10:3778.
  2. Wang, P. et al. (2004) Cell. Mol. Life Sci. 61:2405.
  3. Sunagozaka, H. et al. (2011) Int. J. Cancer 129:1576.
  4. Bortnov, V. et al. (2018) J. Biol. Chem. 293:13166.

Long Name

Stromal Cell-derived Growth Factor SF20

Alternate Names

C19orf10, Ly6elg, MYDGF

Entrez Gene IDs

56005 (Human); 28106 (Mouse)

Gene Symbol

MYDGF

UniProt

Q9CPT4

Additional SF20/MYDGF Products

Product Documents for Recombinant Mouse SF20/MYDGF Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse SF20/MYDGF Protein, CF

For research use only

Citations for Recombinant Mouse SF20/MYDGF Protein, CF

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