Recombinant Mouse sFRP-4 Protein, CF

Catalog # Availability Size / Price Qty
9806-SF-050
Recombinant Mouse sFRP-4 Protein, CF Bioactivity
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Recombinant Mouse sFRP-4 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.2-1.2 ug/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Mouse myeloma cell line, NS0-derived mouse sFRP-4 protein
Ala22-Ser351
Accession #
N-terminal Sequence
Analysis
Ala22
Predicted Molecular Mass
38 kDa
SDS-PAGE
47-63 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9806-SF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Recombinant Mouse sFRP-4 (Catalog # 9806-SF) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50for this effect is 0.2-1.2 µg/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Reconstitution Calculator

Reconstitution Calculator

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Background: sFRP-4

Secreted Frizzled Related Protein-4 (sFRP-4), also known as DDC-4, FrpAP, frpHE and FrzB-2, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled receptors (1, 2). The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Of all the sFRPs, sFRP-4 is most closely related to sFRP-3 (1). Mature mouse sFRP-4 shares 92% and 96% amino acid (aa) sequence identity with human and rat sFRP-4, respectively. sFRP-4 is expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, its role in cancer is likely to be tissue dependent (6). In addition, sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (8).

References
  1. Jones, S.E. and C. Jomary (2002) Bioessays 24:811.
  2. Wolf, V. et al. (1997) FEBS Letters 417:385.
  3. Drake, J.M. et al. (2003) Apoptosis 8:389.
  4. James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452.
  5. Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.
  6. Abu-Jawdeh, G. et al. (1999) Lab Invest. 79:439.
  7. Wong, S.C. et al. (2002) J. Pathol. 196:145.
  8. Berndt, T. et al. (2003) J. Clin. Invest. 112:785.
Long Name
Secreted Frizzled Related Protein 4
Entrez Gene IDs
6424 (Human); 20379 (Mouse); 89803 (Rat)
Alternate Names
DDC-4; Frizzled protein, human endometrium; FRP-4; FRP-AP; frpHE; FRPHEMGC26498; FrzB2; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4; sFRP4; sFRP-4

Citation for Recombinant Mouse sFRP-4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Secreted Frizzled-related protein 4 inhibits the regeneration of hair follicles
    Authors: H Guo, Y Xing, F Deng, K Yang, Y Li
    PeerJ, 2019;6(0):e6153.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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