Recombinant Mouse sFRP-5 Protein, CF

R&D Systems | Catalog # 7195-SF

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse sFRP-5 Protein (7195-SF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_061250

Applications

Bioactivity
View all sFRP-5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse sFRP-5 protein
Ala22-His314, with an N-terminal HA (YPYDVPDYA) tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr (N-terminus of Hemagglutinin tag)

Predicted Molecular Mass

34.3 kDa

SDS-PAGE

39-42 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells.
The ED50 for this effect is 0.600‑7.20 μg/mL.

Scientific Data Images for Recombinant Mouse sFRP-5 Protein, CF

Recombinant Mouse sFRP‑5 Protein Bioactivity.

Recombinant Mouse sFRP-5 (Catalog # 7195-SF) inhibits Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.600-7.20 μg/mL.

Formulation, Preparation, and Storage

7195-SF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. 
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: sFRP-5

Secreted Frizzled Related Protein‑5 (sFRP‑5), also known as SARP‑3, belongs to a family of Wnt‑binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30‑35 kDa in size and contain an N‑terminal Frizzled‑like domain with 10 conserved cysteines and a Netrin‑like C‑terminal domain (1‑3). Mature mouse sFRP‑5 shares 96% and 99% aa sequence identity with human and rat sFRP‑2, respectively (4). During embryonic development, sFRP‑5 is expressed in the anterior visceral endoderm, neural tube, foregut epithelium, and proliferating and prehypertrophic chondrocytes (5‑8). sFRP‑5 activity is required for the development of foregut, liver, ventral pancreas, and somites (6, 7). In the adult, sFRP‑5 is expressed in the retinal pigment epithelium and pancreas (4, 9). sFRP‑5 binds and antagonizes the function of mammalian Wnt‑5a and Wnt‑11 as well as Xenopus Xwnt‑8, resulting in an inhibition of both canonical and non‑canonical Wnt signaling (4, 7, 10). sFRP‑5 down‑regulation is common in breast and gastric cancer cells and is correlated with poor prognosis (11‑13). It functions as a tumor suppressor by inhibiting epithelial‑mesenchymal transition, invasiveness, and tumorigenicity of ovarian cancer cells (14). sFRP‑5 plays an important role in maintaining glucose handling and insulin sensitivity (10). It is secreted by adipocytes and is down‑regulated in mouse models of obesity and type 2 diabetes (10).

References

  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  4. Chang, J. et al. (1999) Hum. Mol. Genet. 8:575.
  5. Leaf, I. et al. (2006) Genesis 44:573.
  6. Satoh, W. et al. (2008) Genesis 46:92.
  7. Li, Y. et al. (2008) Genes Dev. 22:3050.
  8. Witte, F. et al. (2009) Gene Expr. Patterns 9:215.
  9. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  10. Ouchi, N. et al. (2010) Science 329:454.
  11. Veeck, J. et al. (2008) Carcinogenesis 29:991.
  12. Zhao, C. et al. (2009) BMC Cancer 9:224.
  13. Ho, C.M. et al. (2010) Eur. J. Clin. Invest. 40:310.
  14. Su, H.Y. et al. (2010) Int. J. Cancer 127:555.

Long Name

Secreted Frizzled Related Protein 5

Alternate Names

FRP1B, SARP3, sFRP5

Entrez Gene IDs

6425 (Human); 54612 (Mouse)

Gene Symbol

SFRP5

UniProt

NP_061250

Additional sFRP-5 Products

Product Documents for Recombinant Mouse sFRP-5 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse sFRP-5 Protein, CF

For research use only

Related Research Areas

Wnt Family
Wnt Inhibitors

Citations for Recombinant Mouse sFRP-5 Protein, CF

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